Experiment: KB with Trimethoprim 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0781 and Psyr_0782 overlap by 38 nucleotides Psyr_0782 and Psyr_0783 overlap by 4 nucleotides Psyr_0783 and Psyr_0784 are separated by 9 nucleotides
Psyr_0781: Psyr_0781 - Protein-glutamate methylesterase, at 887,924 to 889,000
_0781
Psyr_0782: Psyr_0782 - CheD, at 888,963 to 889,496
_0782
Psyr_0783: Psyr_0783 - MCP methyltransferase, CheR-type, at 889,493 to 890,302
_0783
Psyr_0784: Psyr_0784 - CheW-like protein, at 890,312 to 890,851
_0784
Position (kb)
888
889
890 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 888.219 kb on + strand, within Psyr_0781 at 888.220 kb on - strand, within Psyr_0781 at 888.220 kb on - strand, within Psyr_0781 at 888.220 kb on - strand, within Psyr_0781 at 888.222 kb on + strand, within Psyr_0781 at 888.222 kb on + strand, within Psyr_0781 at 888.222 kb on + strand, within Psyr_0781 at 888.222 kb on + strand, within Psyr_0781 at 888.223 kb on - strand, within Psyr_0781 at 888.223 kb on - strand, within Psyr_0781 at 888.614 kb on + strand, within Psyr_0781 at 888.879 kb on - strand, within Psyr_0781 at 888.941 kb on + strand at 888.953 kb on + strand at 888.953 kb on + strand at 888.977 kb on + strand at 889.038 kb on + strand, within Psyr_0782 at 889.039 kb on - strand, within Psyr_0782 at 889.039 kb on - strand, within Psyr_0782 at 889.039 kb on - strand, within Psyr_0782 at 889.039 kb on - strand, within Psyr_0782 at 889.232 kb on - strand, within Psyr_0782 at 889.275 kb on + strand, within Psyr_0782 at 889.275 kb on + strand, within Psyr_0782 at 889.276 kb on - strand, within Psyr_0782 at 889.297 kb on + strand, within Psyr_0782 at 889.467 kb on + strand at 889.467 kb on + strand at 889.468 kb on - strand at 889.468 kb on - strand at 889.477 kb on - strand at 889.578 kb on + strand, within Psyr_0783 at 889.578 kb on + strand, within Psyr_0783 at 889.578 kb on + strand, within Psyr_0783 at 889.578 kb on + strand, within Psyr_0783 at 889.579 kb on - strand, within Psyr_0783 at 889.579 kb on - strand, within Psyr_0783 at 889.579 kb on - strand, within Psyr_0783 at 889.579 kb on - strand, within Psyr_0783 at 889.579 kb on - strand, within Psyr_0783 at 889.644 kb on + strand, within Psyr_0783 at 889.644 kb on + strand, within Psyr_0783 at 889.794 kb on + strand, within Psyr_0783 at 889.794 kb on + strand, within Psyr_0783 at 889.794 kb on + strand, within Psyr_0783 at 889.794 kb on + strand, within Psyr_0783 at 889.794 kb on + strand, within Psyr_0783 at 889.795 kb on - strand, within Psyr_0783 at 889.795 kb on - strand, within Psyr_0783 at 889.837 kb on + strand, within Psyr_0783 at 889.947 kb on + strand, within Psyr_0783 at 890.064 kb on + strand, within Psyr_0783 at 890.064 kb on + strand, within Psyr_0783 at 890.065 kb on - strand, within Psyr_0783 at 890.151 kb on + strand, within Psyr_0783 at 890.151 kb on + strand, within Psyr_0783 at 890.151 kb on + strand, within Psyr_0783 at 890.151 kb on + strand, within Psyr_0783 at 890.151 kb on + strand, within Psyr_0783 at 890.151 kb on + strand, within Psyr_0783 at 890.167 kb on - strand, within Psyr_0783 at 890.244 kb on + strand at 890.245 kb on - strand at 890.293 kb on + strand at 890.293 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 15.6 ug/ml remove 888,219 + Psyr_0781 0.27 -0.1 888,220 - Psyr_0781 0.27 +1.2 888,220 - Psyr_0781 0.27 -1.7 888,220 - Psyr_0781 0.27 +1.2 888,222 + Psyr_0781 0.28 +0.4 888,222 + Psyr_0781 0.28 -0.5 888,222 + Psyr_0781 0.28 -0.4 888,222 + Psyr_0781 0.28 +0.4 888,223 - Psyr_0781 0.28 -0.3 888,223 - Psyr_0781 0.28 +0.3 888,614 + Psyr_0781 0.64 -0.3 888,879 - Psyr_0781 0.89 +0.3 888,941 + +0.9 888,953 + -0.4 888,953 + +0.2 888,977 + +0.4 889,038 + Psyr_0782 0.14 +1.0 889,039 - Psyr_0782 0.14 -1.5 889,039 - Psyr_0782 0.14 -2.7 889,039 - Psyr_0782 0.14 +0.3 889,039 - Psyr_0782 0.14 +1.1 889,232 - Psyr_0782 0.50 +0.1 889,275 + Psyr_0782 0.58 -0.6 889,275 + Psyr_0782 0.58 +0.1 889,276 - Psyr_0782 0.59 +0.3 889,297 + Psyr_0782 0.63 +0.2 889,467 + +0.2 889,467 + +0.1 889,468 - +0.6 889,468 - +0.1 889,477 - -0.2 889,578 + Psyr_0783 0.10 -0.5 889,578 + Psyr_0783 0.10 +0.3 889,578 + Psyr_0783 0.10 -0.3 889,578 + Psyr_0783 0.10 +0.0 889,579 - Psyr_0783 0.11 +0.7 889,579 - Psyr_0783 0.11 -0.0 889,579 - Psyr_0783 0.11 -0.6 889,579 - Psyr_0783 0.11 +0.6 889,579 - Psyr_0783 0.11 +0.1 889,644 + Psyr_0783 0.19 +0.5 889,644 + Psyr_0783 0.19 +1.1 889,794 + Psyr_0783 0.37 -0.5 889,794 + Psyr_0783 0.37 -0.4 889,794 + Psyr_0783 0.37 +1.9 889,794 + Psyr_0783 0.37 -0.6 889,794 + Psyr_0783 0.37 -0.5 889,795 - Psyr_0783 0.37 -0.4 889,795 - Psyr_0783 0.37 -0.9 889,837 + Psyr_0783 0.42 -1.1 889,947 + Psyr_0783 0.56 +0.5 890,064 + Psyr_0783 0.70 +0.3 890,064 + Psyr_0783 0.70 +0.9 890,065 - Psyr_0783 0.71 +0.3 890,151 + Psyr_0783 0.81 -0.2 890,151 + Psyr_0783 0.81 -0.9 890,151 + Psyr_0783 0.81 +0.5 890,151 + Psyr_0783 0.81 +0.4 890,151 + Psyr_0783 0.81 +0.9 890,151 + Psyr_0783 0.81 -0.1 890,167 - Psyr_0783 0.83 -0.8 890,244 + +1.5 890,245 - -1.9 890,293 + -1.4 890,293 + +0.7
Or see this region's nucleotide sequence