Experiment: KB with Trimethoprim 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0697 and Psyr_0698 are separated by 33 nucleotides Psyr_0698 and Psyr_0699 are separated by 6 nucleotides Psyr_0699 and Psyr_0700 are separated by 146 nucleotides
Psyr_0697: Psyr_0697 - tRNA 2-selenouridine synthase, at 801,532 to 802,632
_0697
Psyr_0698: Psyr_0698 - CDS, at 802,666 to 802,965
_0698
Psyr_0699: Psyr_0699 - CDS, at 802,972 to 803,430
_0699
Psyr_0700: Psyr_0700 - Trans-hexaprenyltranstransferase, at 803,577 to 804,545
_0700
Position (kb) 802
803
804 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 802.001 kb on + strand, within Psyr_0697 at 802.002 kb on - strand, within Psyr_0697 at 802.002 kb on - strand, within Psyr_0697 at 802.002 kb on - strand, within Psyr_0697 at 802.002 kb on - strand, within Psyr_0697 at 802.002 kb on - strand, within Psyr_0697 at 802.002 kb on - strand, within Psyr_0697 at 802.294 kb on + strand, within Psyr_0697 at 802.294 kb on + strand, within Psyr_0697 at 802.294 kb on + strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.389 kb on + strand, within Psyr_0697 at 802.389 kb on + strand, within Psyr_0697 at 802.390 kb on - strand, within Psyr_0697 at 802.519 kb on + strand, within Psyr_0697 at 802.520 kb on - strand, within Psyr_0697 at 802.520 kb on - strand, within Psyr_0697 at 802.520 kb on - strand, within Psyr_0697 at 802.520 kb on - strand, within Psyr_0697 at 802.523 kb on - strand at 802.523 kb on - strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.535 kb on - strand at 802.535 kb on - strand at 802.535 kb on - strand at 802.535 kb on - strand at 802.535 kb on - strand at 802.535 kb on - strand at 802.535 kb on - strand at 802.687 kb on + strand at 802.688 kb on - strand at 802.688 kb on - strand at 803.158 kb on + strand, within Psyr_0699 at 803.158 kb on + strand, within Psyr_0699 at 803.158 kb on + strand, within Psyr_0699 at 803.159 kb on - strand, within Psyr_0699 at 803.159 kb on - strand, within Psyr_0699 at 803.159 kb on - strand, within Psyr_0699 at 803.421 kb on - strand at 803.421 kb on - strand at 803.421 kb on - strand at 803.421 kb on - strand at 803.445 kb on + strand at 803.445 kb on + strand at 803.517 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 15.6 ug/ml remove 802,001 + Psyr_0697 0.43 +0.3 802,002 - Psyr_0697 0.43 +0.7 802,002 - Psyr_0697 0.43 +0.1 802,002 - Psyr_0697 0.43 -1.1 802,002 - Psyr_0697 0.43 +0.5 802,002 - Psyr_0697 0.43 +0.3 802,002 - Psyr_0697 0.43 +0.9 802,294 + Psyr_0697 0.69 -0.8 802,294 + Psyr_0697 0.69 -2.2 802,294 + Psyr_0697 0.69 +0.3 802,295 - Psyr_0697 0.69 -0.7 802,295 - Psyr_0697 0.69 -0.3 802,295 - Psyr_0697 0.69 -0.7 802,295 - Psyr_0697 0.69 +0.4 802,295 - Psyr_0697 0.69 +0.3 802,295 - Psyr_0697 0.69 -0.5 802,295 - Psyr_0697 0.69 -0.7 802,295 - Psyr_0697 0.69 +0.7 802,389 + Psyr_0697 0.78 -0.7 802,389 + Psyr_0697 0.78 +0.3 802,390 - Psyr_0697 0.78 +0.1 802,519 + Psyr_0697 0.90 -0.9 802,520 - Psyr_0697 0.90 -0.7 802,520 - Psyr_0697 0.90 -1.7 802,520 - Psyr_0697 0.90 +0.3 802,520 - Psyr_0697 0.90 +0.9 802,523 - +0.5 802,523 - +0.2 802,534 + +1.9 802,534 + +0.9 802,534 + -1.6 802,534 + -0.1 802,534 + -0.6 802,534 + +1.7 802,534 + +0.5 802,534 + +1.7 802,535 - -0.6 802,535 - -3.4 802,535 - -1.3 802,535 - +0.2 802,535 - -0.9 802,535 - +0.3 802,535 - -0.3 802,687 + +0.3 802,688 - +1.9 802,688 - -1.6 803,158 + Psyr_0699 0.41 +0.6 803,158 + Psyr_0699 0.41 +1.1 803,158 + Psyr_0699 0.41 +1.6 803,159 - Psyr_0699 0.41 +0.0 803,159 - Psyr_0699 0.41 -0.1 803,159 - Psyr_0699 0.41 +0.0 803,421 - -0.6 803,421 - +0.2 803,421 - +0.9 803,421 - +0.7 803,445 + +0.3 803,445 + -1.6 803,517 + -2.4
Or see this region's nucleotide sequence