Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3730 and EX28DRAFT_3731 are separated by 123 nucleotides EX28DRAFT_3731 and EX28DRAFT_3732 overlap by 4 nucleotides EX28DRAFT_3732 and EX28DRAFT_3733 are separated by 75 nucleotides
EX28DRAFT_3730: EX28DRAFT_3730 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 405,190 to 405,981
_3730
EX28DRAFT_3731: EX28DRAFT_3731 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, at 406,105 to 406,584
_3731
EX28DRAFT_3732: EX28DRAFT_3732 - poly(A) polymerase, at 406,581 to 407,945
_3732
EX28DRAFT_3733: EX28DRAFT_3733 - glutamyl-queuosine tRNA(Asp) synthetase, at 408,021 to 408,911
_3733
Position (kb) 406
407
408 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 405.778 kb on - strand, within EX28DRAFT_3730 at 405.902 kb on + strand at 405.903 kb on - strand at 405.909 kb on - strand at 405.959 kb on - strand at 405.981 kb on + strand at 406.029 kb on - strand at 406.052 kb on + strand at 406.052 kb on + strand at 406.052 kb on + strand at 406.053 kb on - strand at 406.053 kb on - strand at 406.056 kb on + strand at 406.955 kb on - strand, within EX28DRAFT_3732 at 406.955 kb on - strand, within EX28DRAFT_3732 at 406.955 kb on - strand, within EX28DRAFT_3732 at 407.910 kb on - strand at 407.991 kb on - strand at 407.991 kb on - strand at 408.025 kb on - strand at 408.138 kb on + strand, within EX28DRAFT_3733 at 408.268 kb on + strand, within EX28DRAFT_3733 at 408.299 kb on - strand, within EX28DRAFT_3733 at 408.306 kb on + strand, within EX28DRAFT_3733 at 408.307 kb on - strand, within EX28DRAFT_3733 at 408.396 kb on - strand, within EX28DRAFT_3733 at 408.397 kb on + strand, within EX28DRAFT_3733 at 408.397 kb on + strand, within EX28DRAFT_3733 at 408.398 kb on - strand, within EX28DRAFT_3733 at 408.398 kb on - strand, within EX28DRAFT_3733 at 408.428 kb on + strand, within EX28DRAFT_3733 at 408.429 kb on - strand, within EX28DRAFT_3733 at 408.429 kb on - strand, within EX28DRAFT_3733 at 408.515 kb on + strand, within EX28DRAFT_3733 at 408.515 kb on + strand, within EX28DRAFT_3733 at 408.515 kb on + strand, within EX28DRAFT_3733 at 408.516 kb on - strand, within EX28DRAFT_3733 at 408.520 kb on - strand, within EX28DRAFT_3733 at 408.616 kb on + strand, within EX28DRAFT_3733 at 408.616 kb on + strand, within EX28DRAFT_3733 at 408.616 kb on + strand, within EX28DRAFT_3733 at 408.617 kb on - strand, within EX28DRAFT_3733 at 408.823 kb on + strand at 408.843 kb on + strand at 408.859 kb on - strand at 408.859 kb on - strand at 408.894 kb on - strand at 408.895 kb on + strand at 408.896 kb on - strand at 408.896 kb on - strand at 408.911 kb on + strand at 408.911 kb on + strand at 408.911 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 405,778 - EX28DRAFT_3730 0.74 -4.0 405,902 + -4.4 405,903 - -2.4 405,909 - -2.4 405,959 - -2.6 405,981 + -0.9 406,029 - +0.5 406,052 + -0.5 406,052 + -0.6 406,052 + +0.0 406,053 - -1.2 406,053 - -0.6 406,056 + +0.0 406,955 - EX28DRAFT_3732 0.27 +1.1 406,955 - EX28DRAFT_3732 0.27 +0.3 406,955 - EX28DRAFT_3732 0.27 +1.8 407,910 - -0.4 407,991 - +0.9 407,991 - +1.1 408,025 - -0.2 408,138 + EX28DRAFT_3733 0.13 -0.1 408,268 + EX28DRAFT_3733 0.28 +0.4 408,299 - EX28DRAFT_3733 0.31 -0.1 408,306 + EX28DRAFT_3733 0.32 -0.2 408,307 - EX28DRAFT_3733 0.32 -0.4 408,396 - EX28DRAFT_3733 0.42 +0.1 408,397 + EX28DRAFT_3733 0.42 +0.0 408,397 + EX28DRAFT_3733 0.42 -0.2 408,398 - EX28DRAFT_3733 0.42 -0.1 408,398 - EX28DRAFT_3733 0.42 -0.3 408,428 + EX28DRAFT_3733 0.46 -0.6 408,429 - EX28DRAFT_3733 0.46 +0.1 408,429 - EX28DRAFT_3733 0.46 -0.2 408,515 + EX28DRAFT_3733 0.55 -0.4 408,515 + EX28DRAFT_3733 0.55 -0.2 408,515 + EX28DRAFT_3733 0.55 +0.9 408,516 - EX28DRAFT_3733 0.56 -0.7 408,520 - EX28DRAFT_3733 0.56 -0.3 408,616 + EX28DRAFT_3733 0.67 -2.9 408,616 + EX28DRAFT_3733 0.67 +0.1 408,616 + EX28DRAFT_3733 0.67 +0.9 408,617 - EX28DRAFT_3733 0.67 -0.2 408,823 + -0.1 408,843 + -0.9 408,859 - -0.0 408,859 - +0.5 408,894 - -0.1 408,895 + -2.0 408,896 - -0.7 408,896 - -0.3 408,911 + -0.2 408,911 + -1.0 408,911 + +0.4
Or see this region's nucleotide sequence