Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2973 and EX28DRAFT_2974 are separated by 341 nucleotides EX28DRAFT_2974 and EX28DRAFT_2975 are separated by 224 nucleotides
EX28DRAFT_2973: EX28DRAFT_2973 - erythrose-4-phosphate dehydrogenase, at 282,874 to 283,893
_2973
EX28DRAFT_2974: EX28DRAFT_2974 - transketolase, bacterial and yeast, at 284,235 to 286,226
_2974
EX28DRAFT_2975: EX28DRAFT_2975 - Zn-dependent protease with chaperone function, at 286,451 to 287,257
_2975
Position (kb)
284
285
286
287 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 283.313 kb on + strand, within EX28DRAFT_2973 at 283.314 kb on - strand, within EX28DRAFT_2973 at 283.746 kb on - strand, within EX28DRAFT_2973 at 283.832 kb on - strand at 283.833 kb on - strand at 283.848 kb on + strand at 283.848 kb on + strand at 283.849 kb on - strand at 283.893 kb on + strand at 284.079 kb on - strand at 284.087 kb on + strand at 284.088 kb on - strand at 284.117 kb on + strand at 284.172 kb on + strand at 284.173 kb on - strand at 284.173 kb on - strand at 284.173 kb on - strand at 284.173 kb on - strand at 284.173 kb on - strand at 284.226 kb on + strand at 284.227 kb on - strand at 284.236 kb on + strand at 284.237 kb on - strand at 284.362 kb on + strand at 284.363 kb on - strand at 284.938 kb on + strand, within EX28DRAFT_2974 at 284.966 kb on + strand, within EX28DRAFT_2974 at 284.966 kb on + strand, within EX28DRAFT_2974 at 285.000 kb on + strand, within EX28DRAFT_2974 at 285.017 kb on - strand, within EX28DRAFT_2974 at 285.030 kb on - strand, within EX28DRAFT_2974 at 285.047 kb on + strand, within EX28DRAFT_2974 at 285.331 kb on - strand, within EX28DRAFT_2974 at 285.682 kb on + strand, within EX28DRAFT_2974 at 285.792 kb on + strand, within EX28DRAFT_2974 at 285.792 kb on + strand, within EX28DRAFT_2974 at 285.793 kb on - strand, within EX28DRAFT_2974 at 285.941 kb on + strand, within EX28DRAFT_2974 at 286.010 kb on + strand, within EX28DRAFT_2974 at 286.137 kb on - strand at 286.215 kb on - strand at 286.332 kb on - strand at 286.332 kb on - strand at 286.332 kb on - strand at 286.332 kb on - strand at 286.332 kb on - strand at 286.423 kb on - strand at 286.423 kb on - strand at 286.423 kb on - strand at 286.423 kb on - strand at 286.427 kb on - strand at 286.468 kb on + strand at 286.469 kb on - strand at 286.469 kb on - strand at 286.477 kb on + strand at 286.515 kb on + strand at 286.657 kb on + strand, within EX28DRAFT_2975 at 286.658 kb on - strand, within EX28DRAFT_2975 at 286.664 kb on - strand, within EX28DRAFT_2975 at 286.699 kb on + strand, within EX28DRAFT_2975 at 286.699 kb on + strand, within EX28DRAFT_2975 at 286.700 kb on - strand, within EX28DRAFT_2975 at 286.700 kb on - strand, within EX28DRAFT_2975 at 286.704 kb on - strand, within EX28DRAFT_2975 at 286.778 kb on + strand, within EX28DRAFT_2975 at 286.779 kb on - strand, within EX28DRAFT_2975 at 286.779 kb on - strand, within EX28DRAFT_2975 at 286.779 kb on - strand, within EX28DRAFT_2975 at 286.779 kb on - strand, within EX28DRAFT_2975 at 286.779 kb on - strand, within EX28DRAFT_2975 at 286.833 kb on - strand, within EX28DRAFT_2975 at 287.038 kb on + strand, within EX28DRAFT_2975 at 287.081 kb on + strand, within EX28DRAFT_2975 at 287.081 kb on + strand, within EX28DRAFT_2975 at 287.081 kb on + strand, within EX28DRAFT_2975 at 287.088 kb on - strand, within EX28DRAFT_2975 at 287.114 kb on - strand, within EX28DRAFT_2975 at 287.221 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 283,313 + EX28DRAFT_2973 0.43 -0.6 283,314 - EX28DRAFT_2973 0.43 +0.0 283,746 - EX28DRAFT_2973 0.85 -0.2 283,832 - +0.9 283,833 - -1.5 283,848 + +0.2 283,848 + -0.6 283,849 - +1.1 283,893 + +0.2 284,079 - -0.2 284,087 + -0.2 284,088 - +0.4 284,117 + +0.2 284,172 + +0.5 284,173 - -0.1 284,173 - -0.0 284,173 - +0.5 284,173 - -0.1 284,173 - -0.6 284,226 + +0.2 284,227 - +1.1 284,236 + +0.4 284,237 - -0.6 284,362 + -3.4 284,363 - -0.1 284,938 + EX28DRAFT_2974 0.35 -2.5 284,966 + EX28DRAFT_2974 0.37 -2.0 284,966 + EX28DRAFT_2974 0.37 -1.6 285,000 + EX28DRAFT_2974 0.38 -1.2 285,017 - EX28DRAFT_2974 0.39 -0.7 285,030 - EX28DRAFT_2974 0.40 -2.4 285,047 + EX28DRAFT_2974 0.41 -1.2 285,331 - EX28DRAFT_2974 0.55 -1.6 285,682 + EX28DRAFT_2974 0.73 -1.0 285,792 + EX28DRAFT_2974 0.78 -2.0 285,792 + EX28DRAFT_2974 0.78 -0.9 285,793 - EX28DRAFT_2974 0.78 -1.2 285,941 + EX28DRAFT_2974 0.86 -1.2 286,010 + EX28DRAFT_2974 0.89 -1.5 286,137 - +0.6 286,215 - +0.3 286,332 - -1.4 286,332 - -1.0 286,332 - +0.2 286,332 - +0.3 286,332 - +0.2 286,423 - +1.0 286,423 - +0.5 286,423 - +1.5 286,423 - +0.5 286,427 - +0.4 286,468 + -0.3 286,469 - +0.0 286,469 - +0.2 286,477 + +0.0 286,515 + -0.4 286,657 + EX28DRAFT_2975 0.26 +0.9 286,658 - EX28DRAFT_2975 0.26 -0.4 286,664 - EX28DRAFT_2975 0.26 +0.2 286,699 + EX28DRAFT_2975 0.31 +0.5 286,699 + EX28DRAFT_2975 0.31 -0.4 286,700 - EX28DRAFT_2975 0.31 -0.4 286,700 - EX28DRAFT_2975 0.31 +1.2 286,704 - EX28DRAFT_2975 0.31 +0.2 286,778 + EX28DRAFT_2975 0.41 +0.3 286,779 - EX28DRAFT_2975 0.41 -0.0 286,779 - EX28DRAFT_2975 0.41 +0.3 286,779 - EX28DRAFT_2975 0.41 -0.4 286,779 - EX28DRAFT_2975 0.41 +0.1 286,779 - EX28DRAFT_2975 0.41 +1.7 286,833 - EX28DRAFT_2975 0.47 -0.3 287,038 + EX28DRAFT_2975 0.73 +0.2 287,081 + EX28DRAFT_2975 0.78 +0.2 287,081 + EX28DRAFT_2975 0.78 -0.8 287,081 + EX28DRAFT_2975 0.78 +0.0 287,088 - EX28DRAFT_2975 0.79 +0.2 287,114 - EX28DRAFT_2975 0.82 -0.5 287,221 + -0.1
Or see this region's nucleotide sequence