Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2945 and EX28DRAFT_2946 are separated by 101 nucleotides EX28DRAFT_2946 and EX28DRAFT_2947 are separated by 6 nucleotides EX28DRAFT_2947 and EX28DRAFT_2948 overlap by 20 nucleotides EX28DRAFT_2948 and EX28DRAFT_2949 are separated by 294 nucleotides
EX28DRAFT_2945: EX28DRAFT_2945 - tyrosine recombinase XerD, at 256,008 to 256,904
_2945
EX28DRAFT_2946: EX28DRAFT_2946 - flavodoxin, long chain, at 257,006 to 257,527
_2946
EX28DRAFT_2947: EX28DRAFT_2947 - Protein of unknown function (DUF1434), at 257,534 to 257,944
_2947
EX28DRAFT_2948: EX28DRAFT_2948 - Uncharacterized conserved protein, at 257,925 to 258,191
_2948
EX28DRAFT_2949: EX28DRAFT_2949 - folate-binding protein YgfZ, at 258,486 to 259,466
_2949
Position (kb)
257
258 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 256.170 kb on + strand, within EX28DRAFT_2945 at 256.477 kb on + strand, within EX28DRAFT_2945 at 256.478 kb on - strand, within EX28DRAFT_2945 at 256.543 kb on - strand, within EX28DRAFT_2945 at 256.936 kb on + strand at 256.996 kb on + strand at 257.001 kb on - strand at 257.011 kb on + strand at 257.012 kb on - strand at 257.025 kb on - strand at 257.141 kb on + strand, within EX28DRAFT_2946 at 257.142 kb on - strand, within EX28DRAFT_2946 at 257.187 kb on + strand, within EX28DRAFT_2946 at 257.206 kb on + strand, within EX28DRAFT_2946 at 257.206 kb on + strand, within EX28DRAFT_2946 at 257.357 kb on + strand, within EX28DRAFT_2946 at 257.597 kb on - strand, within EX28DRAFT_2947 at 257.738 kb on + strand, within EX28DRAFT_2947 at 257.744 kb on - strand, within EX28DRAFT_2947 at 257.830 kb on + strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.833 kb on - strand, within EX28DRAFT_2947 at 257.833 kb on - strand, within EX28DRAFT_2947 at 257.888 kb on + strand, within EX28DRAFT_2947 at 257.967 kb on + strand, within EX28DRAFT_2948 at 258.097 kb on + strand, within EX28DRAFT_2948 at 258.097 kb on + strand, within EX28DRAFT_2948 at 258.098 kb on - strand, within EX28DRAFT_2948 at 258.098 kb on - strand, within EX28DRAFT_2948 at 258.098 kb on - strand, within EX28DRAFT_2948 at 258.098 kb on - strand, within EX28DRAFT_2948 at 258.103 kb on + strand, within EX28DRAFT_2948 at 258.104 kb on - strand, within EX28DRAFT_2948 at 258.128 kb on + strand, within EX28DRAFT_2948 at 258.208 kb on + strand at 258.208 kb on + strand at 258.209 kb on - strand at 258.209 kb on - strand at 258.245 kb on + strand at 258.246 kb on - strand at 258.269 kb on - strand at 258.269 kb on - strand at 258.273 kb on + strand at 258.305 kb on - strand at 258.334 kb on - strand at 258.361 kb on + strand at 258.441 kb on + strand at 258.441 kb on + strand at 258.441 kb on + strand at 258.447 kb on + strand at 258.454 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 256,170 + EX28DRAFT_2945 0.18 -1.1 256,477 + EX28DRAFT_2945 0.52 +0.3 256,478 - EX28DRAFT_2945 0.52 -0.7 256,543 - EX28DRAFT_2945 0.60 -4.0 256,936 + -0.2 256,996 + +0.1 257,001 - -1.2 257,011 + -1.0 257,012 - -2.0 257,025 - -1.0 257,141 + EX28DRAFT_2946 0.26 -1.7 257,142 - EX28DRAFT_2946 0.26 -2.2 257,187 + EX28DRAFT_2946 0.35 -0.3 257,206 + EX28DRAFT_2946 0.38 -1.8 257,206 + EX28DRAFT_2946 0.38 -1.0 257,357 + EX28DRAFT_2946 0.67 -2.4 257,597 - EX28DRAFT_2947 0.15 +0.7 257,738 + EX28DRAFT_2947 0.50 -1.3 257,744 - EX28DRAFT_2947 0.51 +0.4 257,830 + EX28DRAFT_2947 0.72 +0.0 257,831 - EX28DRAFT_2947 0.72 +0.6 257,831 - EX28DRAFT_2947 0.72 +0.2 257,831 - EX28DRAFT_2947 0.72 +0.1 257,831 - EX28DRAFT_2947 0.72 +0.5 257,833 - EX28DRAFT_2947 0.73 +1.2 257,833 - EX28DRAFT_2947 0.73 +1.3 257,888 + EX28DRAFT_2947 0.86 -0.3 257,967 + EX28DRAFT_2948 0.16 +2.0 258,097 + EX28DRAFT_2948 0.64 +2.8 258,097 + EX28DRAFT_2948 0.64 +1.2 258,098 - EX28DRAFT_2948 0.65 +2.5 258,098 - EX28DRAFT_2948 0.65 +2.4 258,098 - EX28DRAFT_2948 0.65 +2.5 258,098 - EX28DRAFT_2948 0.65 +2.7 258,103 + EX28DRAFT_2948 0.67 +2.4 258,104 - EX28DRAFT_2948 0.67 +2.7 258,128 + EX28DRAFT_2948 0.76 +2.0 258,208 + +0.8 258,208 + +1.2 258,209 - +1.9 258,209 - +0.2 258,245 + -0.2 258,246 - +0.8 258,269 - +0.0 258,269 - +0.2 258,273 + +0.1 258,305 - +0.4 258,334 - -0.1 258,361 + -0.6 258,441 + +0.7 258,441 + -0.3 258,441 + +0.1 258,447 + +0.2 258,454 + +0.3
Or see this region's nucleotide sequence