Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2916 and EX28DRAFT_2917 are separated by 121 nucleotides EX28DRAFT_2917 and EX28DRAFT_2918 overlap by 6 nucleotides EX28DRAFT_2918 and EX28DRAFT_2919 are separated by 88 nucleotides
EX28DRAFT_2916: EX28DRAFT_2916 - diaminopimelate decarboxylase, at 226,401 to 227,663
_2916
EX28DRAFT_2917: EX28DRAFT_2917 - Transcriptional regulator, at 227,785 to 228,711
_2917
EX28DRAFT_2918: EX28DRAFT_2918 - aspartate racemase, at 228,706 to 229,398
_2918
EX28DRAFT_2919: EX28DRAFT_2919 - Chain length determinant protein, at 229,487 to 230,611
_2919
Position (kb) 227
228
229 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 227.072 kb on - strand, within EX28DRAFT_2916 at 227.096 kb on + strand, within EX28DRAFT_2916 at 227.131 kb on - strand, within EX28DRAFT_2916 at 227.153 kb on + strand, within EX28DRAFT_2916 at 227.154 kb on - strand, within EX28DRAFT_2916 at 227.154 kb on - strand, within EX28DRAFT_2916 at 227.288 kb on + strand, within EX28DRAFT_2916 at 227.289 kb on - strand, within EX28DRAFT_2916 at 227.292 kb on + strand, within EX28DRAFT_2916 at 227.293 kb on - strand, within EX28DRAFT_2916 at 227.484 kb on - strand, within EX28DRAFT_2916 at 227.678 kb on - strand at 227.764 kb on + strand at 227.871 kb on + strand at 227.871 kb on + strand at 227.871 kb on + strand at 228.365 kb on - strand, within EX28DRAFT_2917 at 228.391 kb on + strand, within EX28DRAFT_2917 at 228.412 kb on + strand, within EX28DRAFT_2917 at 228.413 kb on + strand, within EX28DRAFT_2917 at 228.568 kb on - strand, within EX28DRAFT_2917 at 228.686 kb on + strand at 228.867 kb on - strand, within EX28DRAFT_2918 at 228.869 kb on + strand, within EX28DRAFT_2918 at 228.919 kb on - strand, within EX28DRAFT_2918 at 229.019 kb on + strand, within EX28DRAFT_2918 at 229.020 kb on - strand, within EX28DRAFT_2918 at 229.020 kb on - strand, within EX28DRAFT_2918 at 229.089 kb on + strand, within EX28DRAFT_2918 at 229.123 kb on - strand, within EX28DRAFT_2918 at 229.146 kb on + strand, within EX28DRAFT_2918 at 229.146 kb on + strand, within EX28DRAFT_2918 at 229.152 kb on + strand, within EX28DRAFT_2918 at 229.409 kb on + strand at 229.409 kb on + strand at 229.409 kb on + strand at 229.410 kb on - strand at 229.410 kb on - strand at 229.444 kb on + strand at 229.445 kb on - strand at 229.445 kb on - strand at 229.460 kb on - strand at 229.666 kb on + strand, within EX28DRAFT_2919 at 229.705 kb on - strand, within EX28DRAFT_2919 at 229.707 kb on + strand, within EX28DRAFT_2919 at 229.707 kb on + strand, within EX28DRAFT_2919 at 229.707 kb on + strand, within EX28DRAFT_2919 at 229.707 kb on + strand, within EX28DRAFT_2919
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 227,072 - EX28DRAFT_2916 0.53 -4.1 227,096 + EX28DRAFT_2916 0.55 -4.2 227,131 - EX28DRAFT_2916 0.58 -3.4 227,153 + EX28DRAFT_2916 0.60 -1.9 227,154 - EX28DRAFT_2916 0.60 -1.3 227,154 - EX28DRAFT_2916 0.60 -3.6 227,288 + EX28DRAFT_2916 0.70 -3.2 227,289 - EX28DRAFT_2916 0.70 -3.0 227,292 + EX28DRAFT_2916 0.71 -2.7 227,293 - EX28DRAFT_2916 0.71 -2.1 227,484 - EX28DRAFT_2916 0.86 -3.5 227,678 - -1.3 227,764 + +0.2 227,871 + -3.6 227,871 + -3.0 227,871 + -1.2 228,365 - EX28DRAFT_2917 0.63 -3.9 228,391 + EX28DRAFT_2917 0.65 -3.4 228,412 + EX28DRAFT_2917 0.68 -1.1 228,413 + EX28DRAFT_2917 0.68 -2.4 228,568 - EX28DRAFT_2917 0.84 -2.6 228,686 + -0.7 228,867 - EX28DRAFT_2918 0.23 -0.1 228,869 + EX28DRAFT_2918 0.24 +0.8 228,919 - EX28DRAFT_2918 0.31 -0.3 229,019 + EX28DRAFT_2918 0.45 +0.1 229,020 - EX28DRAFT_2918 0.45 +0.1 229,020 - EX28DRAFT_2918 0.45 +0.5 229,089 + EX28DRAFT_2918 0.55 +0.1 229,123 - EX28DRAFT_2918 0.60 -0.2 229,146 + EX28DRAFT_2918 0.63 +0.1 229,146 + EX28DRAFT_2918 0.63 +0.4 229,152 + EX28DRAFT_2918 0.64 -0.3 229,409 + -0.2 229,409 + -0.9 229,409 + -0.1 229,410 - -0.6 229,410 - +0.4 229,444 + +0.3 229,445 - +0.9 229,445 - +0.2 229,460 - -0.2 229,666 + EX28DRAFT_2919 0.16 -1.0 229,705 - EX28DRAFT_2919 0.19 -0.3 229,707 + EX28DRAFT_2919 0.20 -0.6 229,707 + EX28DRAFT_2919 0.20 +0.4 229,707 + EX28DRAFT_2919 0.20 -0.0 229,707 + EX28DRAFT_2919 0.20 +0.2
Or see this region's nucleotide sequence