Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2770 and EX28DRAFT_2771 overlap by 14 nucleotides EX28DRAFT_2771 and EX28DRAFT_2772 are separated by 54 nucleotides EX28DRAFT_2772 and EX28DRAFT_2773 are separated by 72 nucleotides EX28DRAFT_2773 and EX28DRAFT_2774 are separated by 8 nucleotides
EX28DRAFT_2770: EX28DRAFT_2770 - 2-isopropylmalate synthase, yeast type, at 78,763 to 80,418
_2770
EX28DRAFT_2771: EX28DRAFT_2771 - AraC-type DNA-binding domain-containing proteins, at 80,405 to 81,169
_2771
EX28DRAFT_2772: EX28DRAFT_2772 - S-ribosylhomocysteine lyase (EC 4.4.1.21)/quorum-sensing autoinducer 2 (AI-2) synthesis protein LuxS, at 81,224 to 81,739
_2772
EX28DRAFT_2773: EX28DRAFT_2773 - RNA, at 81,812 to 81,887
_2773
EX28DRAFT_2774: EX28DRAFT_2774 - glutamate-cysteine ligase (EC 6.3.2.2), at 81,896 to 83,440
_2774
Position (kb)
81
82 Strain fitness (log2 ratio)
-1
0
1
2 at 80.261 kb on - strand at 80.261 kb on - strand at 80.271 kb on + strand at 80.271 kb on + strand at 80.272 kb on - strand at 80.272 kb on - strand at 80.272 kb on - strand at 80.309 kb on - strand at 80.417 kb on - strand at 80.583 kb on - strand, within EX28DRAFT_2771 at 80.782 kb on - strand, within EX28DRAFT_2771 at 80.782 kb on - strand, within EX28DRAFT_2771 at 80.782 kb on - strand, within EX28DRAFT_2771 at 81.225 kb on + strand at 81.225 kb on + strand at 81.226 kb on - strand at 81.226 kb on - strand at 81.226 kb on - strand at 81.348 kb on + strand, within EX28DRAFT_2772 at 81.348 kb on + strand, within EX28DRAFT_2772 at 81.349 kb on - strand, within EX28DRAFT_2772 at 81.352 kb on + strand, within EX28DRAFT_2772 at 81.363 kb on + strand, within EX28DRAFT_2772 at 81.477 kb on + strand, within EX28DRAFT_2772 at 81.477 kb on + strand, within EX28DRAFT_2772 at 81.577 kb on + strand, within EX28DRAFT_2772 at 81.578 kb on - strand, within EX28DRAFT_2772 at 81.683 kb on + strand, within EX28DRAFT_2772 at 81.725 kb on - strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.732 kb on - strand at 81.739 kb on + strand at 81.740 kb on - strand at 81.774 kb on + strand at 81.789 kb on + strand at 81.790 kb on - strand at 81.808 kb on + strand at 81.809 kb on - strand at 81.857 kb on - strand, within EX28DRAFT_2773 at 81.857 kb on - strand, within EX28DRAFT_2773 at 81.859 kb on - strand, within EX28DRAFT_2773 at 81.898 kb on - strand at 82.090 kb on + strand, within EX28DRAFT_2774 at 82.298 kb on - strand, within EX28DRAFT_2774 at 82.467 kb on - strand, within EX28DRAFT_2774 at 82.476 kb on + strand, within EX28DRAFT_2774 at 82.677 kb on + strand, within EX28DRAFT_2774 at 82.700 kb on - strand, within EX28DRAFT_2774 at 82.724 kb on - strand, within EX28DRAFT_2774
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 80,261 - +0.3 80,261 - -0.3 80,271 + -0.4 80,271 + -0.8 80,272 - -0.4 80,272 - +0.9 80,272 - -0.2 80,309 - -0.2 80,417 - -0.2 80,583 - EX28DRAFT_2771 0.23 +0.5 80,782 - EX28DRAFT_2771 0.49 +0.2 80,782 - EX28DRAFT_2771 0.49 -0.1 80,782 - EX28DRAFT_2771 0.49 +0.3 81,225 + -0.3 81,225 + +0.2 81,226 - -0.1 81,226 - +0.1 81,226 - +1.0 81,348 + EX28DRAFT_2772 0.24 -0.7 81,348 + EX28DRAFT_2772 0.24 -0.2 81,349 - EX28DRAFT_2772 0.24 -0.7 81,352 + EX28DRAFT_2772 0.25 +0.3 81,363 + EX28DRAFT_2772 0.27 -0.5 81,477 + EX28DRAFT_2772 0.49 -0.5 81,477 + EX28DRAFT_2772 0.49 +0.9 81,577 + EX28DRAFT_2772 0.68 -0.1 81,578 - EX28DRAFT_2772 0.69 +0.1 81,683 + EX28DRAFT_2772 0.89 -0.2 81,725 - +0.3 81,728 + -0.6 81,728 + +0.1 81,728 + -0.3 81,728 + +0.4 81,728 + +0.7 81,732 - -0.7 81,739 + -0.2 81,740 - +1.2 81,774 + +0.5 81,789 + -0.4 81,790 - +0.6 81,808 + +0.2 81,809 - +0.3 81,857 - EX28DRAFT_2773 0.59 +0.7 81,857 - EX28DRAFT_2773 0.59 +0.2 81,859 - EX28DRAFT_2773 0.62 +0.2 81,898 - +0.7 82,090 + EX28DRAFT_2774 0.13 +0.6 82,298 - EX28DRAFT_2774 0.26 +2.0 82,467 - EX28DRAFT_2774 0.37 +1.4 82,476 + EX28DRAFT_2774 0.38 +0.4 82,677 + EX28DRAFT_2774 0.51 +0.0 82,700 - EX28DRAFT_2774 0.52 +1.0 82,724 - EX28DRAFT_2774 0.54 +0.6
Or see this region's nucleotide sequence