Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0169 and EX28DRAFT_0170 are separated by 227 nucleotides EX28DRAFT_0170 and EX28DRAFT_0171 are separated by 83 nucleotides EX28DRAFT_0171 and EX28DRAFT_0172 are separated by 81 nucleotides EX28DRAFT_0172 and EX28DRAFT_0173 are separated by 41 nucleotides
EX28DRAFT_0169: EX28DRAFT_0169 - Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase, at 117,999 to 119,429
_0169
EX28DRAFT_0170: EX28DRAFT_0170 - uracil phosphoribosyltransferase (EC 2.4.2.9), at 119,657 to 120,283
_0170
EX28DRAFT_0171: EX28DRAFT_0171 - uracil-xanthine permease, at 120,367 to 121,656
_0171
EX28DRAFT_0172: EX28DRAFT_0172 - regulatory inactivation of DnaA Hda protein, at 121,738 to 122,454
_0172
EX28DRAFT_0173: EX28DRAFT_0173 - Leucyl aminopeptidase, at 122,496 to 123,914
_0173
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-1
0
1 at 119.393 kb on + strand at 119.394 kb on - strand at 119.394 kb on - strand at 119.394 kb on - strand at 119.427 kb on + strand at 119.465 kb on + strand at 119.466 kb on - strand at 119.466 kb on - strand at 119.466 kb on - strand at 119.471 kb on + strand at 119.471 kb on + strand at 119.481 kb on + strand at 119.482 kb on - strand at 119.567 kb on + strand at 119.568 kb on - strand at 119.568 kb on - strand at 119.568 kb on - strand at 119.568 kb on - strand at 119.592 kb on + strand at 119.960 kb on + strand, within EX28DRAFT_0170 at 119.993 kb on + strand, within EX28DRAFT_0170 at 119.994 kb on - strand, within EX28DRAFT_0170 at 119.994 kb on - strand, within EX28DRAFT_0170 at 120.110 kb on - strand, within EX28DRAFT_0170 at 120.118 kb on + strand, within EX28DRAFT_0170 at 120.119 kb on - strand, within EX28DRAFT_0170 at 120.191 kb on + strand, within EX28DRAFT_0170 at 120.192 kb on - strand, within EX28DRAFT_0170 at 120.192 kb on - strand, within EX28DRAFT_0170 at 120.192 kb on - strand, within EX28DRAFT_0170 at 120.230 kb on + strand at 120.296 kb on - strand at 120.296 kb on - strand at 120.346 kb on + strand at 120.346 kb on + strand at 120.347 kb on - strand at 120.347 kb on - strand at 120.349 kb on + strand at 120.350 kb on - strand at 120.366 kb on + strand at 120.367 kb on - strand at 120.367 kb on - strand at 120.426 kb on + strand at 120.535 kb on + strand, within EX28DRAFT_0171 at 120.591 kb on + strand, within EX28DRAFT_0171 at 120.648 kb on + strand, within EX28DRAFT_0171 at 120.649 kb on - strand, within EX28DRAFT_0171 at 120.649 kb on - strand, within EX28DRAFT_0171 at 120.792 kb on + strand, within EX28DRAFT_0171 at 120.803 kb on + strand, within EX28DRAFT_0171 at 120.804 kb on - strand, within EX28DRAFT_0171 at 120.844 kb on - strand, within EX28DRAFT_0171 at 121.021 kb on + strand, within EX28DRAFT_0171 at 121.022 kb on - strand, within EX28DRAFT_0171 at 121.023 kb on + strand, within EX28DRAFT_0171 at 121.047 kb on + strand, within EX28DRAFT_0171 at 121.125 kb on + strand, within EX28DRAFT_0171 at 121.229 kb on - strand, within EX28DRAFT_0171 at 121.229 kb on - strand, within EX28DRAFT_0171 at 121.257 kb on + strand, within EX28DRAFT_0171 at 121.268 kb on - strand, within EX28DRAFT_0171 at 121.272 kb on + strand, within EX28DRAFT_0171 at 121.273 kb on - strand, within EX28DRAFT_0171 at 121.391 kb on - strand, within EX28DRAFT_0171 at 121.445 kb on - strand, within EX28DRAFT_0171 at 121.730 kb on + strand at 122.452 kb on + strand at 122.453 kb on - strand at 122.453 kb on - strand at 122.497 kb on + strand at 122.556 kb on - strand at 122.588 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 119,393 + +0.5 119,394 - -0.7 119,394 - +0.3 119,394 - +1.3 119,427 + +0.1 119,465 + +0.4 119,466 - -0.9 119,466 - -0.3 119,466 - +0.4 119,471 + -0.4 119,471 + -0.3 119,481 + -0.1 119,482 - -0.4 119,567 + -0.0 119,568 - +0.2 119,568 - +0.7 119,568 - -0.2 119,568 - -0.2 119,592 + -0.2 119,960 + EX28DRAFT_0170 0.48 +0.3 119,993 + EX28DRAFT_0170 0.54 +0.3 119,994 - EX28DRAFT_0170 0.54 +0.1 119,994 - EX28DRAFT_0170 0.54 +0.2 120,110 - EX28DRAFT_0170 0.72 +0.1 120,118 + EX28DRAFT_0170 0.74 +0.6 120,119 - EX28DRAFT_0170 0.74 +0.5 120,191 + EX28DRAFT_0170 0.85 -1.2 120,192 - EX28DRAFT_0170 0.85 +0.5 120,192 - EX28DRAFT_0170 0.85 +0.1 120,192 - EX28DRAFT_0170 0.85 -0.1 120,230 + +0.4 120,296 - +0.3 120,296 - -0.1 120,346 + -0.1 120,346 + +0.6 120,347 - +1.5 120,347 - +0.6 120,349 + -0.7 120,350 - +0.3 120,366 + +0.7 120,367 - +0.2 120,367 - +0.5 120,426 + +0.5 120,535 + EX28DRAFT_0171 0.13 +0.9 120,591 + EX28DRAFT_0171 0.17 +0.6 120,648 + EX28DRAFT_0171 0.22 -0.5 120,649 - EX28DRAFT_0171 0.22 -1.1 120,649 - EX28DRAFT_0171 0.22 +0.5 120,792 + EX28DRAFT_0171 0.33 -0.7 120,803 + EX28DRAFT_0171 0.34 +0.5 120,804 - EX28DRAFT_0171 0.34 +0.6 120,844 - EX28DRAFT_0171 0.37 -0.1 121,021 + EX28DRAFT_0171 0.51 +0.3 121,022 - EX28DRAFT_0171 0.51 +0.5 121,023 + EX28DRAFT_0171 0.51 +0.5 121,047 + EX28DRAFT_0171 0.53 +0.0 121,125 + EX28DRAFT_0171 0.59 -0.1 121,229 - EX28DRAFT_0171 0.67 +0.2 121,229 - EX28DRAFT_0171 0.67 +0.5 121,257 + EX28DRAFT_0171 0.69 +0.4 121,268 - EX28DRAFT_0171 0.70 +0.6 121,272 + EX28DRAFT_0171 0.70 +0.9 121,273 - EX28DRAFT_0171 0.70 -0.1 121,391 - EX28DRAFT_0171 0.79 -0.2 121,445 - EX28DRAFT_0171 0.84 +0.1 121,730 + -1.4 122,452 + +0.9 122,453 - -0.4 122,453 - +0.2 122,497 + +0.9 122,556 - +0.2 122,588 + +0.2
Or see this region's nucleotide sequence