Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_0123

Experiment: MS_media with m-arsenite 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_0122 and EX28DRAFT_0123 are separated by 286 nucleotidesEX28DRAFT_0123 and EX28DRAFT_0124 are separated by 1 nucleotides EX28DRAFT_0122: EX28DRAFT_0122 - PTS system, glucose-like IIB component, at 59,743 to 61,287 _0122 EX28DRAFT_0123: EX28DRAFT_0123 - Large extracellular alpha-helical protein, at 61,574 to 66,526 _0123 EX28DRAFT_0124: EX28DRAFT_0124 - penicillin-binding protein 1C, at 66,528 to 68,852 _0124 Position (kb) 61 62 63 64 65 66 67Strain fitness (log2 ratio) -2 -1 0 1 2at 60.618 kb on + strand, within EX28DRAFT_0122at 60.619 kb on - strand, within EX28DRAFT_0122at 60.821 kb on + strand, within EX28DRAFT_0122at 60.830 kb on + strand, within EX28DRAFT_0122at 61.161 kb on - strandat 61.161 kb on - strandat 61.161 kb on - strandat 61.174 kb on - strandat 61.174 kb on - strandat 61.226 kb on + strandat 61.227 kb on + strandat 61.227 kb on + strandat 61.227 kb on + strandat 61.227 kb on + strandat 61.228 kb on - strandat 61.228 kb on - strandat 61.341 kb on - strandat 61.448 kb on - strandat 61.526 kb on + strandat 61.526 kb on + strandat 61.526 kb on + strandat 61.526 kb on + strandat 61.526 kb on + strandat 61.527 kb on - strandat 61.527 kb on - strandat 61.527 kb on - strandat 61.527 kb on - strandat 61.530 kb on + strandat 61.569 kb on - strandat 61.624 kb on + strandat 61.625 kb on - strandat 61.736 kb on - strandat 61.781 kb on - strandat 62.219 kb on - strand, within EX28DRAFT_0123at 62.273 kb on + strand, within EX28DRAFT_0123at 62.308 kb on + strand, within EX28DRAFT_0123at 62.342 kb on - strand, within EX28DRAFT_0123at 62.345 kb on - strand, within EX28DRAFT_0123at 62.370 kb on - strand, within EX28DRAFT_0123at 62.370 kb on - strand, within EX28DRAFT_0123at 62.399 kb on + strand, within EX28DRAFT_0123at 62.400 kb on - strand, within EX28DRAFT_0123at 62.400 kb on - strand, within EX28DRAFT_0123at 62.406 kb on - strand, within EX28DRAFT_0123at 62.666 kb on - strand, within EX28DRAFT_0123at 62.911 kb on + strand, within EX28DRAFT_0123at 62.911 kb on + strand, within EX28DRAFT_0123at 62.912 kb on - strand, within EX28DRAFT_0123at 62.912 kb on - strand, within EX28DRAFT_0123at 62.945 kb on - strand, within EX28DRAFT_0123at 62.979 kb on + strand, within EX28DRAFT_0123at 62.979 kb on + strand, within EX28DRAFT_0123at 62.980 kb on - strand, within EX28DRAFT_0123at 62.980 kb on - strand, within EX28DRAFT_0123at 63.026 kb on - strand, within EX28DRAFT_0123at 63.085 kb on + strand, within EX28DRAFT_0123at 63.099 kb on - strand, within EX28DRAFT_0123at 63.099 kb on - strand, within EX28DRAFT_0123at 63.101 kb on + strand, within EX28DRAFT_0123at 63.102 kb on - strand, within EX28DRAFT_0123at 63.127 kb on + strand, within EX28DRAFT_0123at 63.127 kb on + strand, within EX28DRAFT_0123at 63.128 kb on - strand, within EX28DRAFT_0123at 63.447 kb on - strand, within EX28DRAFT_0123at 63.698 kb on - strand, within EX28DRAFT_0123at 63.789 kb on - strand, within EX28DRAFT_0123at 63.866 kb on + strand, within EX28DRAFT_0123at 63.884 kb on - strand, within EX28DRAFT_0123at 63.884 kb on - strand, within EX28DRAFT_0123at 63.884 kb on - strand, within EX28DRAFT_0123at 64.038 kb on - strand, within EX28DRAFT_0123at 64.069 kb on + strand, within EX28DRAFT_0123at 64.282 kb on + strand, within EX28DRAFT_0123at 64.282 kb on + strand, within EX28DRAFT_0123at 64.352 kb on + strand, within EX28DRAFT_0123at 64.353 kb on - strand, within EX28DRAFT_0123at 64.353 kb on - strand, within EX28DRAFT_0123at 64.353 kb on - strand, within EX28DRAFT_0123at 64.357 kb on + strand, within EX28DRAFT_0123at 64.361 kb on + strand, within EX28DRAFT_0123at 64.361 kb on + strand, within EX28DRAFT_0123at 64.362 kb on - strand, within EX28DRAFT_0123at 64.643 kb on - strand, within EX28DRAFT_0123at 64.819 kb on + strand, within EX28DRAFT_0123at 64.820 kb on - strand, within EX28DRAFT_0123at 64.966 kb on + strand, within EX28DRAFT_0123at 65.017 kb on + strand, within EX28DRAFT_0123at 65.017 kb on + strand, within EX28DRAFT_0123at 65.018 kb on - strand, within EX28DRAFT_0123at 65.039 kb on - strand, within EX28DRAFT_0123at 65.093 kb on + strand, within EX28DRAFT_0123at 65.094 kb on - strand, within EX28DRAFT_0123at 65.165 kb on + strand, within EX28DRAFT_0123at 65.166 kb on - strand, within EX28DRAFT_0123at 65.379 kb on + strand, within EX28DRAFT_0123at 65.423 kb on + strand, within EX28DRAFT_0123at 65.760 kb on - strand, within EX28DRAFT_0123at 65.760 kb on - strand, within EX28DRAFT_0123at 65.760 kb on - strand, within EX28DRAFT_0123at 65.785 kb on + strand, within EX28DRAFT_0123at 65.785 kb on + strand, within EX28DRAFT_0123at 65.785 kb on + strand, within EX28DRAFT_0123at 65.941 kb on + strand, within EX28DRAFT_0123at 66.118 kb on + strandat 66.119 kb on - strandat 66.119 kb on - strandat 66.404 kb on + strandat 66.434 kb on + strandat 66.463 kb on + strandat 66.463 kb on + strandat 66.463 kb on + strandat 66.463 kb on + strandat 66.464 kb on - strandat 66.464 kb on - strandat 66.464 kb on - strandat 66.464 kb on - strandat 66.464 kb on - strandat 66.464 kb on - strandat 66.464 kb on - strandat 66.464 kb on - strandat 66.464 kb on - strandat 66.467 kb on + strandat 66.468 kb on - strandat 66.809 kb on - strand, within EX28DRAFT_0124at 67.006 kb on - strand, within EX28DRAFT_0124at 67.066 kb on - strand, within EX28DRAFT_0124at 67.090 kb on + strand, within EX28DRAFT_0124at 67.090 kb on + strand, within EX28DRAFT_0124at 67.090 kb on + strand, within EX28DRAFT_0124at 67.090 kb on + strand, within EX28DRAFT_0124at 67.091 kb on - strand, within EX28DRAFT_0124at 67.091 kb on - strand, within EX28DRAFT_0124at 67.091 kb on - strand, within EX28DRAFT_0124at 67.096 kb on - strand, within EX28DRAFT_0124at 67.144 kb on + strand, within EX28DRAFT_0124at 67.240 kb on + strand, within EX28DRAFT_0124at 67.458 kb on - strand, within EX28DRAFT_0124at 67.491 kb on - strand, within EX28DRAFT_0124at 67.493 kb on + strand, within EX28DRAFT_0124

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Per-strain Table

Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM
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60,618 + EX28DRAFT_0122 0.57 -0.6
60,619 - EX28DRAFT_0122 0.57 +0.6
60,821 + EX28DRAFT_0122 0.70 +0.2
60,830 + EX28DRAFT_0122 0.70 +0.0
61,161 - +1.3
61,161 - +0.3
61,161 - +0.1
61,174 - -0.5
61,174 - +0.3
61,226 + +0.5
61,227 + -0.2
61,227 + +0.5
61,227 + -1.5
61,227 + -0.6
61,228 - +0.4
61,228 - +0.3
61,341 - +0.2
61,448 - +0.2
61,526 + +0.3
61,526 + +0.1
61,526 + -0.1
61,526 + -0.0
61,526 + +0.7
61,527 - +0.1
61,527 - +1.2
61,527 - -0.3
61,527 - +0.3
61,530 + +0.3
61,569 - +0.2
61,624 + -0.4
61,625 - +0.0
61,736 - -0.2
61,781 - +0.4
62,219 - EX28DRAFT_0123 0.13 -0.3
62,273 + EX28DRAFT_0123 0.14 -0.2
62,308 + EX28DRAFT_0123 0.15 +0.2
62,342 - EX28DRAFT_0123 0.16 +0.1
62,345 - EX28DRAFT_0123 0.16 +0.3
62,370 - EX28DRAFT_0123 0.16 -0.2
62,370 - EX28DRAFT_0123 0.16 -0.4
62,399 + EX28DRAFT_0123 0.17 -0.3
62,400 - EX28DRAFT_0123 0.17 -0.8
62,400 - EX28DRAFT_0123 0.17 -0.8
62,406 - EX28DRAFT_0123 0.17 +0.5
62,666 - EX28DRAFT_0123 0.22 -0.1
62,911 + EX28DRAFT_0123 0.27 +0.1
62,911 + EX28DRAFT_0123 0.27 +0.1
62,912 - EX28DRAFT_0123 0.27 +0.1
62,912 - EX28DRAFT_0123 0.27 +0.0
62,945 - EX28DRAFT_0123 0.28 +0.4
62,979 + EX28DRAFT_0123 0.28 -0.4
62,979 + EX28DRAFT_0123 0.28 -1.0
62,980 - EX28DRAFT_0123 0.28 -0.3
62,980 - EX28DRAFT_0123 0.28 -0.5
63,026 - EX28DRAFT_0123 0.29 +0.4
63,085 + EX28DRAFT_0123 0.31 +0.4
63,099 - EX28DRAFT_0123 0.31 -0.1
63,099 - EX28DRAFT_0123 0.31 -0.4
63,101 + EX28DRAFT_0123 0.31 -0.2
63,102 - EX28DRAFT_0123 0.31 +0.4
63,127 + EX28DRAFT_0123 0.31 -0.3
63,127 + EX28DRAFT_0123 0.31 +0.6
63,128 - EX28DRAFT_0123 0.31 -0.2
63,447 - EX28DRAFT_0123 0.38 +0.2
63,698 - EX28DRAFT_0123 0.43 +0.2
63,789 - EX28DRAFT_0123 0.45 -0.1
63,866 + EX28DRAFT_0123 0.46 -0.0
63,884 - EX28DRAFT_0123 0.47 -0.4
63,884 - EX28DRAFT_0123 0.47 -0.4
63,884 - EX28DRAFT_0123 0.47 -0.4
64,038 - EX28DRAFT_0123 0.50 -0.5
64,069 + EX28DRAFT_0123 0.50 -0.1
64,282 + EX28DRAFT_0123 0.55 +0.2
64,282 + EX28DRAFT_0123 0.55 +0.3
64,352 + EX28DRAFT_0123 0.56 -0.3
64,353 - EX28DRAFT_0123 0.56 +0.3
64,353 - EX28DRAFT_0123 0.56 -0.1
64,353 - EX28DRAFT_0123 0.56 -0.1
64,357 + EX28DRAFT_0123 0.56 -0.2
64,361 + EX28DRAFT_0123 0.56 -0.2
64,361 + EX28DRAFT_0123 0.56 -0.3
64,362 - EX28DRAFT_0123 0.56 -0.6
64,643 - EX28DRAFT_0123 0.62 -0.0
64,819 + EX28DRAFT_0123 0.66 -0.7
64,820 - EX28DRAFT_0123 0.66 -0.4
64,966 + EX28DRAFT_0123 0.68 -0.3
65,017 + EX28DRAFT_0123 0.70 +0.1
65,017 + EX28DRAFT_0123 0.70 +0.3
65,018 - EX28DRAFT_0123 0.70 -0.3
65,039 - EX28DRAFT_0123 0.70 -1.1
65,093 + EX28DRAFT_0123 0.71 +0.3
65,094 - EX28DRAFT_0123 0.71 -0.2
65,165 + EX28DRAFT_0123 0.73 -0.5
65,166 - EX28DRAFT_0123 0.73 +0.7
65,379 + EX28DRAFT_0123 0.77 +0.2
65,423 + EX28DRAFT_0123 0.78 -0.3
65,760 - EX28DRAFT_0123 0.85 +0.2
65,760 - EX28DRAFT_0123 0.85 -0.3
65,760 - EX28DRAFT_0123 0.85 +0.5
65,785 + EX28DRAFT_0123 0.85 +1.5
65,785 + EX28DRAFT_0123 0.85 +0.1
65,785 + EX28DRAFT_0123 0.85 +0.1
65,941 + EX28DRAFT_0123 0.88 -0.3
66,118 + -0.3
66,119 - -0.2
66,119 - -0.0
66,404 + +0.0
66,434 + +0.3
66,463 + -0.9
66,463 + +0.2
66,463 + +0.3
66,463 + +0.6
66,464 - +0.1
66,464 - +0.6
66,464 - -0.4
66,464 - -0.3
66,464 - -0.2
66,464 - +1.8
66,464 - -1.8
66,464 - -0.5
66,464 - -0.4
66,467 + +0.6
66,468 - +0.1
66,809 - EX28DRAFT_0124 0.12 -0.6
67,006 - EX28DRAFT_0124 0.21 +0.6
67,066 - EX28DRAFT_0124 0.23 -0.4
67,090 + EX28DRAFT_0124 0.24 +0.2
67,090 + EX28DRAFT_0124 0.24 +0.5
67,090 + EX28DRAFT_0124 0.24 -0.8
67,090 + EX28DRAFT_0124 0.24 +0.6
67,091 - EX28DRAFT_0124 0.24 +0.2
67,091 - EX28DRAFT_0124 0.24 -0.5
67,091 - EX28DRAFT_0124 0.24 +1.0
67,096 - EX28DRAFT_0124 0.24 +0.2
67,144 + EX28DRAFT_0124 0.26 -0.6
67,240 + EX28DRAFT_0124 0.31 +0.7
67,458 - EX28DRAFT_0124 0.40 -0.8
67,491 - EX28DRAFT_0124 0.41 +0.4
67,493 + EX28DRAFT_0124 0.42 +0.7

Or see this region's nucleotide sequence