Experiment: m.b. Polymyxin B sulfate 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0060 and Echvi_0061 are separated by 229 nucleotides Echvi_0061 and Echvi_0062 overlap by 11 nucleotides Echvi_0062 and Echvi_0063 are separated by 263 nucleotides
Echvi_0060: Echvi_0060 - Predicted nucleoside-diphosphate sugar epimerases, at 57,956 to 59,830
_0060
Echvi_0061: Echvi_0061 - Sugar transferases involved in lipopolysaccharide synthesis, at 60,060 to 60,662
_0061
Echvi_0062: Echvi_0062 - Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis, at 60,652 to 61,785
_0062
Echvi_0063: Echvi_0063 - UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase, at 62,049 to 63,203
_0063
Position (kb) 60
61
62 Strain fitness (log2 ratio) -2
-1
0
1
2 at 59.682 kb on - strand at 59.682 kb on - strand at 59.734 kb on + strand at 59.767 kb on - strand at 59.789 kb on + strand at 59.832 kb on - strand at 59.835 kb on - strand at 59.935 kb on + strand at 59.935 kb on + strand at 59.936 kb on - strand at 60.009 kb on - strand at 60.029 kb on + strand at 60.045 kb on + strand at 60.061 kb on + strand at 60.061 kb on + strand at 60.062 kb on - strand at 60.084 kb on + strand at 60.085 kb on - strand at 60.085 kb on - strand at 60.085 kb on - strand at 60.085 kb on - strand at 60.085 kb on - strand at 60.088 kb on + strand at 60.088 kb on + strand at 60.088 kb on + strand at 60.088 kb on + strand at 60.089 kb on - strand at 60.089 kb on - strand at 60.089 kb on - strand at 60.165 kb on - strand, within Echvi_0061 at 60.172 kb on - strand, within Echvi_0061 at 60.184 kb on + strand, within Echvi_0061 at 60.185 kb on - strand, within Echvi_0061 at 60.185 kb on - strand, within Echvi_0061 at 60.185 kb on - strand, within Echvi_0061 at 60.185 kb on - strand, within Echvi_0061 at 60.238 kb on - strand, within Echvi_0061 at 60.238 kb on - strand, within Echvi_0061 at 60.298 kb on + strand, within Echvi_0061 at 60.298 kb on + strand, within Echvi_0061 at 60.310 kb on + strand, within Echvi_0061 at 60.311 kb on - strand, within Echvi_0061 at 60.411 kb on - strand, within Echvi_0061 at 60.465 kb on + strand, within Echvi_0061 at 60.466 kb on - strand, within Echvi_0061 at 60.484 kb on - strand, within Echvi_0061 at 60.484 kb on - strand, within Echvi_0061 at 60.525 kb on - strand, within Echvi_0061 at 60.567 kb on + strand, within Echvi_0061 at 60.567 kb on + strand, within Echvi_0061 at 60.568 kb on - strand, within Echvi_0061 at 60.568 kb on - strand, within Echvi_0061 at 60.586 kb on - strand, within Echvi_0061 at 60.589 kb on - strand, within Echvi_0061 at 60.595 kb on - strand, within Echvi_0061 at 60.595 kb on - strand, within Echvi_0061 at 60.595 kb on - strand, within Echvi_0061 at 60.605 kb on + strand at 60.605 kb on + strand at 60.606 kb on - strand at 60.646 kb on - strand at 60.653 kb on + strand at 60.653 kb on + strand at 60.654 kb on - strand at 60.654 kb on - strand at 60.658 kb on + strand at 60.688 kb on + strand at 60.688 kb on + strand at 60.692 kb on - strand at 60.692 kb on - strand at 60.722 kb on + strand at 60.723 kb on - strand at 60.746 kb on - strand at 60.767 kb on + strand, within Echvi_0062 at 60.791 kb on + strand, within Echvi_0062 at 60.792 kb on - strand, within Echvi_0062 at 60.795 kb on - strand, within Echvi_0062 at 60.821 kb on - strand, within Echvi_0062 at 60.829 kb on - strand, within Echvi_0062 at 60.855 kb on + strand, within Echvi_0062 at 60.912 kb on + strand, within Echvi_0062 at 60.912 kb on + strand, within Echvi_0062 at 60.912 kb on + strand, within Echvi_0062 at 60.933 kb on + strand, within Echvi_0062 at 60.952 kb on - strand, within Echvi_0062 at 60.967 kb on + strand, within Echvi_0062 at 61.036 kb on + strand, within Echvi_0062 at 61.053 kb on + strand, within Echvi_0062 at 61.060 kb on + strand, within Echvi_0062 at 61.060 kb on + strand, within Echvi_0062 at 61.060 kb on + strand, within Echvi_0062 at 61.060 kb on + strand, within Echvi_0062 at 61.060 kb on + strand, within Echvi_0062 at 61.060 kb on + strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.064 kb on - strand, within Echvi_0062 at 61.080 kb on + strand, within Echvi_0062 at 61.092 kb on + strand, within Echvi_0062 at 61.092 kb on + strand, within Echvi_0062 at 61.093 kb on - strand, within Echvi_0062 at 61.093 kb on - strand, within Echvi_0062 at 61.093 kb on - strand, within Echvi_0062 at 61.093 kb on - strand, within Echvi_0062 at 61.093 kb on - strand, within Echvi_0062 at 61.093 kb on - strand, within Echvi_0062 at 61.108 kb on - strand, within Echvi_0062 at 61.108 kb on - strand, within Echvi_0062 at 61.116 kb on - strand, within Echvi_0062 at 61.142 kb on - strand, within Echvi_0062 at 61.183 kb on - strand, within Echvi_0062 at 61.238 kb on + strand, within Echvi_0062 at 61.279 kb on + strand, within Echvi_0062 at 61.280 kb on - strand, within Echvi_0062 at 61.329 kb on - strand, within Echvi_0062 at 61.331 kb on + strand, within Echvi_0062 at 61.391 kb on - strand, within Echvi_0062 at 61.500 kb on + strand, within Echvi_0062 at 61.625 kb on + strand, within Echvi_0062 at 61.626 kb on - strand, within Echvi_0062 at 61.635 kb on + strand, within Echvi_0062 at 61.651 kb on - strand, within Echvi_0062 at 61.651 kb on - strand, within Echvi_0062 at 61.651 kb on - strand, within Echvi_0062 at 61.669 kb on + strand, within Echvi_0062 at 61.670 kb on - strand, within Echvi_0062 at 61.685 kb on + strand at 61.685 kb on + strand at 61.686 kb on - strand at 61.686 kb on - strand at 61.812 kb on - strand at 61.815 kb on - strand at 61.815 kb on - strand at 61.937 kb on - strand at 61.955 kb on + strand at 61.955 kb on + strand at 61.956 kb on - strand at 61.998 kb on + strand at 62.030 kb on + strand at 62.032 kb on + strand at 62.050 kb on + strand at 62.060 kb on + strand at 62.075 kb on + strand at 62.075 kb on + strand at 62.076 kb on - strand at 62.111 kb on + strand at 62.111 kb on + strand at 62.111 kb on + strand at 62.136 kb on + strand at 62.196 kb on + strand, within Echvi_0063 at 62.211 kb on + strand, within Echvi_0063 at 62.263 kb on - strand, within Echvi_0063 at 62.292 kb on - strand, within Echvi_0063 at 62.301 kb on + strand, within Echvi_0063 at 62.328 kb on - strand, within Echvi_0063 at 62.328 kb on - strand, within Echvi_0063 at 62.344 kb on - strand, within Echvi_0063 at 62.388 kb on - strand, within Echvi_0063 at 62.412 kb on + strand, within Echvi_0063 at 62.412 kb on + strand, within Echvi_0063 at 62.423 kb on + strand, within Echvi_0063 at 62.423 kb on + strand, within Echvi_0063 at 62.440 kb on - strand, within Echvi_0063 at 62.440 kb on - strand, within Echvi_0063 at 62.463 kb on + strand, within Echvi_0063 at 62.507 kb on + strand, within Echvi_0063 at 62.507 kb on + strand, within Echvi_0063 at 62.508 kb on - strand, within Echvi_0063 at 62.522 kb on + strand, within Echvi_0063 at 62.529 kb on + strand, within Echvi_0063 at 62.529 kb on + strand, within Echvi_0063 at 62.529 kb on + strand, within Echvi_0063 at 62.530 kb on - strand, within Echvi_0063 at 62.530 kb on - strand, within Echvi_0063 at 62.530 kb on - strand, within Echvi_0063 at 62.538 kb on + strand, within Echvi_0063 at 62.538 kb on + strand, within Echvi_0063 at 62.551 kb on - strand, within Echvi_0063 at 62.600 kb on + strand, within Echvi_0063 at 62.601 kb on - strand, within Echvi_0063 at 62.602 kb on + strand, within Echvi_0063 at 62.617 kb on - strand, within Echvi_0063 at 62.654 kb on - strand, within Echvi_0063 at 62.657 kb on + strand, within Echvi_0063 at 62.657 kb on + strand, within Echvi_0063 at 62.657 kb on + strand, within Echvi_0063 at 62.658 kb on - strand, within Echvi_0063 at 62.658 kb on - strand, within Echvi_0063 at 62.658 kb on - strand, within Echvi_0063 at 62.658 kb on - strand, within Echvi_0063 at 62.658 kb on - strand, within Echvi_0063 at 62.660 kb on + strand, within Echvi_0063 at 62.702 kb on + strand, within Echvi_0063 at 62.705 kb on - strand, within Echvi_0063 at 62.708 kb on + strand, within Echvi_0063 at 62.708 kb on + strand, within Echvi_0063 at 62.708 kb on + strand, within Echvi_0063 at 62.708 kb on + strand, within Echvi_0063 at 62.708 kb on + strand, within Echvi_0063 at 62.708 kb on + strand, within Echvi_0063 at 62.709 kb on - strand, within Echvi_0063 at 62.709 kb on - strand, within Echvi_0063 at 62.709 kb on - strand, within Echvi_0063 at 62.709 kb on - strand, within Echvi_0063 at 62.712 kb on + strand, within Echvi_0063 at 62.712 kb on + strand, within Echvi_0063 at 62.713 kb on - strand, within Echvi_0063 at 62.744 kb on + strand, within Echvi_0063 at 62.782 kb on - strand, within Echvi_0063
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 1 mg/ml remove 59,682 - +1.1 59,682 - +1.8 59,734 + -0.1 59,767 - -0.9 59,789 + -0.8 59,832 - +1.9 59,835 - -0.8 59,935 + -0.5 59,935 + +1.1 59,936 - +0.5 60,009 - +1.1 60,029 + +0.5 60,045 + -0.9 60,061 + +0.5 60,061 + +0.7 60,062 - +0.5 60,084 + -0.3 60,085 - +1.2 60,085 - +0.7 60,085 - -0.1 60,085 - -0.5 60,085 - -1.0 60,088 + +0.6 60,088 + +0.6 60,088 + -0.4 60,088 + +0.1 60,089 - -0.3 60,089 - -0.0 60,089 - -0.8 60,165 - Echvi_0061 0.17 +0.8 60,172 - Echvi_0061 0.19 -2.0 60,184 + Echvi_0061 0.21 -1.3 60,185 - Echvi_0061 0.21 -1.3 60,185 - Echvi_0061 0.21 -0.0 60,185 - Echvi_0061 0.21 +0.3 60,185 - Echvi_0061 0.21 +0.5 60,238 - Echvi_0061 0.30 -0.8 60,238 - Echvi_0061 0.30 +0.2 60,298 + Echvi_0061 0.39 -0.9 60,298 + Echvi_0061 0.39 +0.6 60,310 + Echvi_0061 0.41 -0.4 60,311 - Echvi_0061 0.42 -0.6 60,411 - Echvi_0061 0.58 -0.2 60,465 + Echvi_0061 0.67 -1.9 60,466 - Echvi_0061 0.67 -1.3 60,484 - Echvi_0061 0.70 -0.3 60,484 - Echvi_0061 0.70 -0.7 60,525 - Echvi_0061 0.77 +0.3 60,567 + Echvi_0061 0.84 -0.8 60,567 + Echvi_0061 0.84 +0.5 60,568 - Echvi_0061 0.84 -0.6 60,568 - Echvi_0061 0.84 -0.2 60,586 - Echvi_0061 0.87 +0.8 60,589 - Echvi_0061 0.88 +0.4 60,595 - Echvi_0061 0.89 -1.4 60,595 - Echvi_0061 0.89 -0.1 60,595 - Echvi_0061 0.89 +0.3 60,605 + +0.1 60,605 + +1.1 60,606 - -0.5 60,646 - +0.4 60,653 + +0.1 60,653 + -0.4 60,654 - -1.0 60,654 - -1.4 60,658 + +0.8 60,688 + +0.2 60,688 + -0.9 60,692 - +1.3 60,692 - -0.2 60,722 + +0.7 60,723 - -0.8 60,746 - -0.2 60,767 + Echvi_0062 0.10 -2.0 60,791 + Echvi_0062 0.12 +0.8 60,792 - Echvi_0062 0.12 -1.3 60,795 - Echvi_0062 0.13 -1.4 60,821 - Echvi_0062 0.15 -1.0 60,829 - Echvi_0062 0.16 -0.1 60,855 + Echvi_0062 0.18 -0.5 60,912 + Echvi_0062 0.23 -2.5 60,912 + Echvi_0062 0.23 +0.6 60,912 + Echvi_0062 0.23 +2.2 60,933 + Echvi_0062 0.25 +0.2 60,952 - Echvi_0062 0.26 +0.2 60,967 + Echvi_0062 0.28 +0.3 61,036 + Echvi_0062 0.34 -0.5 61,053 + Echvi_0062 0.35 +0.1 61,060 + Echvi_0062 0.36 -2.3 61,060 + Echvi_0062 0.36 -0.4 61,060 + Echvi_0062 0.36 -1.6 61,060 + Echvi_0062 0.36 -0.8 61,060 + Echvi_0062 0.36 -0.4 61,060 + Echvi_0062 0.36 +1.4 61,061 - Echvi_0062 0.36 +0.2 61,061 - Echvi_0062 0.36 -0.6 61,061 - Echvi_0062 0.36 +0.5 61,061 - Echvi_0062 0.36 +1.4 61,061 - Echvi_0062 0.36 +0.2 61,061 - Echvi_0062 0.36 -0.4 61,061 - Echvi_0062 0.36 +0.9 61,061 - Echvi_0062 0.36 -0.2 61,064 - Echvi_0062 0.36 +0.8 61,080 + Echvi_0062 0.38 +0.6 61,092 + Echvi_0062 0.39 -0.0 61,092 + Echvi_0062 0.39 +0.8 61,093 - Echvi_0062 0.39 -0.8 61,093 - Echvi_0062 0.39 +0.2 61,093 - Echvi_0062 0.39 +0.2 61,093 - Echvi_0062 0.39 -0.6 61,093 - Echvi_0062 0.39 +0.1 61,093 - Echvi_0062 0.39 +0.2 61,108 - Echvi_0062 0.40 -0.2 61,108 - Echvi_0062 0.40 -0.1 61,116 - Echvi_0062 0.41 -1.0 61,142 - Echvi_0062 0.43 +0.9 61,183 - Echvi_0062 0.47 -0.2 61,238 + Echvi_0062 0.52 +0.1 61,279 + Echvi_0062 0.55 +0.6 61,280 - Echvi_0062 0.55 -0.1 61,329 - Echvi_0062 0.60 -0.8 61,331 + Echvi_0062 0.60 -1.1 61,391 - Echvi_0062 0.65 +0.1 61,500 + Echvi_0062 0.75 -0.9 61,625 + Echvi_0062 0.86 -0.7 61,626 - Echvi_0062 0.86 +0.4 61,635 + Echvi_0062 0.87 +0.1 61,651 - Echvi_0062 0.88 -0.0 61,651 - Echvi_0062 0.88 +1.2 61,651 - Echvi_0062 0.88 +0.2 61,669 + Echvi_0062 0.90 +2.4 61,670 - Echvi_0062 0.90 -0.7 61,685 + +0.0 61,685 + -1.6 61,686 - +0.8 61,686 - +0.6 61,812 - -0.0 61,815 - +0.6 61,815 - -0.2 61,937 - -1.0 61,955 + +0.8 61,955 + +0.3 61,956 - +0.1 61,998 + -0.4 62,030 + +1.0 62,032 + +0.4 62,050 + +0.9 62,060 + +0.8 62,075 + -0.2 62,075 + -0.1 62,076 - -0.0 62,111 + +1.0 62,111 + +0.4 62,111 + +0.8 62,136 + +0.6 62,196 + Echvi_0063 0.13 +0.8 62,211 + Echvi_0063 0.14 +0.3 62,263 - Echvi_0063 0.19 -0.4 62,292 - Echvi_0063 0.21 +0.8 62,301 + Echvi_0063 0.22 -0.1 62,328 - Echvi_0063 0.24 -0.7 62,328 - Echvi_0063 0.24 +0.0 62,344 - Echvi_0063 0.26 -0.3 62,388 - Echvi_0063 0.29 -0.1 62,412 + Echvi_0063 0.31 +0.3 62,412 + Echvi_0063 0.31 +1.0 62,423 + Echvi_0063 0.32 -0.9 62,423 + Echvi_0063 0.32 +0.5 62,440 - Echvi_0063 0.34 +0.5 62,440 - Echvi_0063 0.34 +1.0 62,463 + Echvi_0063 0.36 +2.6 62,507 + Echvi_0063 0.40 +0.4 62,507 + Echvi_0063 0.40 +0.7 62,508 - Echvi_0063 0.40 +0.4 62,522 + Echvi_0063 0.41 +2.1 62,529 + Echvi_0063 0.42 +0.4 62,529 + Echvi_0063 0.42 -2.1 62,529 + Echvi_0063 0.42 +0.9 62,530 - Echvi_0063 0.42 -0.4 62,530 - Echvi_0063 0.42 -0.2 62,530 - Echvi_0063 0.42 +0.6 62,538 + Echvi_0063 0.42 +2.0 62,538 + Echvi_0063 0.42 +0.3 62,551 - Echvi_0063 0.43 +0.3 62,600 + Echvi_0063 0.48 -0.8 62,601 - Echvi_0063 0.48 -1.4 62,602 + Echvi_0063 0.48 +0.9 62,617 - Echvi_0063 0.49 -0.9 62,654 - Echvi_0063 0.52 +0.6 62,657 + Echvi_0063 0.53 +1.3 62,657 + Echvi_0063 0.53 +0.2 62,657 + Echvi_0063 0.53 -0.9 62,658 - Echvi_0063 0.53 -0.5 62,658 - Echvi_0063 0.53 -0.3 62,658 - Echvi_0063 0.53 -1.0 62,658 - Echvi_0063 0.53 -0.2 62,658 - Echvi_0063 0.53 +0.0 62,660 + Echvi_0063 0.53 -0.5 62,702 + Echvi_0063 0.57 +0.4 62,705 - Echvi_0063 0.57 -0.6 62,708 + Echvi_0063 0.57 +0.3 62,708 + Echvi_0063 0.57 +1.2 62,708 + Echvi_0063 0.57 +1.0 62,708 + Echvi_0063 0.57 +0.2 62,708 + Echvi_0063 0.57 +0.4 62,708 + Echvi_0063 0.57 +0.1 62,709 - Echvi_0063 0.57 +1.5 62,709 - Echvi_0063 0.57 -1.8 62,709 - Echvi_0063 0.57 +0.7 62,709 - Echvi_0063 0.57 -0.7 62,712 + Echvi_0063 0.57 +0.1 62,712 + Echvi_0063 0.57 +0.2 62,713 - Echvi_0063 0.57 +0.6 62,744 + Echvi_0063 0.60 -1.1 62,782 - Echvi_0063 0.63 -0.2
Or see this region's nucleotide sequence