Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3899 and EX28DRAFT_3900 are separated by 37 nucleotides EX28DRAFT_3900 and EX28DRAFT_3901 are separated by 3 nucleotides
EX28DRAFT_3899: EX28DRAFT_3899 - (p)ppGpp synthetase, RelA/SpoT family, at 106,117 to 108,231
_3899
EX28DRAFT_3900: EX28DRAFT_3900 - SpoU rRNA Methylase family/SpoU, rRNA methylase, C-terminal, at 108,269 to 108,925
_3900
EX28DRAFT_3901: EX28DRAFT_3901 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 108,929 to 111,010
_3901
Position (kb)
108
109 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 107.922 kb on + strand, within EX28DRAFT_3899 at 107.923 kb on - strand, within EX28DRAFT_3899 at 107.977 kb on - strand, within EX28DRAFT_3899 at 108.010 kb on - strand, within EX28DRAFT_3899 at 108.018 kb on + strand, within EX28DRAFT_3899 at 108.018 kb on + strand, within EX28DRAFT_3899 at 108.018 kb on + strand, within EX28DRAFT_3899 at 108.171 kb on + strand at 108.230 kb on - strand at 108.230 kb on - strand at 108.251 kb on + strand at 108.259 kb on + strand at 108.319 kb on + strand at 108.319 kb on + strand at 108.319 kb on + strand at 108.320 kb on - strand at 108.320 kb on - strand at 108.711 kb on - strand, within EX28DRAFT_3900 at 108.746 kb on + strand, within EX28DRAFT_3900 at 108.747 kb on - strand, within EX28DRAFT_3900 at 109.109 kb on + strand at 109.325 kb on + strand, within EX28DRAFT_3901 at 109.558 kb on + strand, within EX28DRAFT_3901 at 109.566 kb on + strand, within EX28DRAFT_3901 at 109.614 kb on + strand, within EX28DRAFT_3901 at 109.681 kb on - strand, within EX28DRAFT_3901 at 109.730 kb on - strand, within EX28DRAFT_3901 at 109.879 kb on + strand, within EX28DRAFT_3901
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 107,922 + EX28DRAFT_3899 0.85 -0.6 107,923 - EX28DRAFT_3899 0.85 -1.0 107,977 - EX28DRAFT_3899 0.88 -0.8 108,010 - EX28DRAFT_3899 0.90 -1.1 108,018 + EX28DRAFT_3899 0.90 -0.6 108,018 + EX28DRAFT_3899 0.90 +0.2 108,018 + EX28DRAFT_3899 0.90 -0.8 108,171 + -0.1 108,230 - -0.9 108,230 - -0.7 108,251 + +0.0 108,259 + +0.3 108,319 + +0.0 108,319 + +0.4 108,319 + -1.1 108,320 - -4.2 108,320 - -2.5 108,711 - EX28DRAFT_3900 0.67 -3.7 108,746 + EX28DRAFT_3900 0.73 -0.9 108,747 - EX28DRAFT_3900 0.73 -1.5 109,109 + -2.5 109,325 + EX28DRAFT_3901 0.19 -1.8 109,558 + EX28DRAFT_3901 0.30 -1.4 109,566 + EX28DRAFT_3901 0.31 -3.2 109,614 + EX28DRAFT_3901 0.33 -1.8 109,681 - EX28DRAFT_3901 0.36 -3.9 109,730 - EX28DRAFT_3901 0.38 -0.7 109,879 + EX28DRAFT_3901 0.46 -1.2
Or see this region's nucleotide sequence