Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3657 and EX28DRAFT_3658 are separated by 44 nucleotides EX28DRAFT_3658 and EX28DRAFT_3659 overlap by 17 nucleotides EX28DRAFT_3659 and EX28DRAFT_3660 overlap by 25 nucleotides
EX28DRAFT_3657: EX28DRAFT_3657 - Uncharacterized membrane-associated protein, at 324,557 to 325,324
_3657
EX28DRAFT_3658: EX28DRAFT_3658 - thiamine ABC transporter, ATP-binding protein, at 325,369 to 326,070
_3658
EX28DRAFT_3659: EX28DRAFT_3659 - thiamine ABC transporter, permease protein, at 326,054 to 327,664
_3659
EX28DRAFT_3660: EX28DRAFT_3660 - thiamine ABC transporter, periplasmic binding protein, at 327,640 to 328,623
_3660
Position (kb)
326
327
328 Strain fitness (log2 ratio)
-2
-1
0
1 at 325.088 kb on - strand, within EX28DRAFT_3657 at 325.190 kb on + strand, within EX28DRAFT_3657 at 325.191 kb on - strand, within EX28DRAFT_3657 at 325.503 kb on + strand, within EX28DRAFT_3658 at 325.864 kb on - strand, within EX28DRAFT_3658 at 325.944 kb on + strand, within EX28DRAFT_3658 at 325.945 kb on - strand, within EX28DRAFT_3658 at 325.945 kb on - strand, within EX28DRAFT_3658 at 326.098 kb on + strand at 326.102 kb on + strand at 326.187 kb on + strand at 326.489 kb on + strand, within EX28DRAFT_3659 at 326.870 kb on - strand, within EX28DRAFT_3659 at 326.953 kb on - strand, within EX28DRAFT_3659 at 326.974 kb on - strand, within EX28DRAFT_3659 at 327.052 kb on + strand, within EX28DRAFT_3659 at 327.215 kb on - strand, within EX28DRAFT_3659 at 327.341 kb on + strand, within EX28DRAFT_3659 at 327.627 kb on - strand at 327.670 kb on + strand at 327.671 kb on - strand at 327.769 kb on - strand, within EX28DRAFT_3660 at 327.769 kb on - strand, within EX28DRAFT_3660 at 327.779 kb on + strand, within EX28DRAFT_3660 at 327.903 kb on - strand, within EX28DRAFT_3660 at 327.932 kb on + strand, within EX28DRAFT_3660 at 327.932 kb on + strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.936 kb on + strand, within EX28DRAFT_3660 at 327.936 kb on + strand, within EX28DRAFT_3660 at 327.937 kb on - strand, within EX28DRAFT_3660 at 327.937 kb on - strand, within EX28DRAFT_3660 at 327.937 kb on - strand, within EX28DRAFT_3660 at 327.978 kb on + strand, within EX28DRAFT_3660 at 327.978 kb on + strand, within EX28DRAFT_3660 at 327.979 kb on - strand, within EX28DRAFT_3660 at 327.981 kb on - strand, within EX28DRAFT_3660 at 327.981 kb on - strand, within EX28DRAFT_3660 at 327.981 kb on - strand, within EX28DRAFT_3660 at 328.238 kb on + strand, within EX28DRAFT_3660 at 328.253 kb on + strand, within EX28DRAFT_3660 at 328.545 kb on - strand at 328.545 kb on - strand at 328.550 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 325,088 - EX28DRAFT_3657 0.69 +0.3 325,190 + EX28DRAFT_3657 0.82 +0.4 325,191 - EX28DRAFT_3657 0.83 +0.6 325,503 + EX28DRAFT_3658 0.19 -0.1 325,864 - EX28DRAFT_3658 0.71 +0.3 325,944 + EX28DRAFT_3658 0.82 -0.2 325,945 - EX28DRAFT_3658 0.82 -1.1 325,945 - EX28DRAFT_3658 0.82 -1.3 326,098 + +0.6 326,102 + +0.2 326,187 + +0.1 326,489 + EX28DRAFT_3659 0.27 +0.0 326,870 - EX28DRAFT_3659 0.51 -0.9 326,953 - EX28DRAFT_3659 0.56 -0.7 326,974 - EX28DRAFT_3659 0.57 -2.1 327,052 + EX28DRAFT_3659 0.62 +0.5 327,215 - EX28DRAFT_3659 0.72 -0.2 327,341 + EX28DRAFT_3659 0.80 +0.4 327,627 - -0.2 327,670 + -0.9 327,671 - -0.4 327,769 - EX28DRAFT_3660 0.13 -0.2 327,769 - EX28DRAFT_3660 0.13 -0.3 327,779 + EX28DRAFT_3660 0.14 -0.2 327,903 - EX28DRAFT_3660 0.27 -0.6 327,932 + EX28DRAFT_3660 0.30 -0.5 327,932 + EX28DRAFT_3660 0.30 -1.1 327,933 - EX28DRAFT_3660 0.30 -0.8 327,933 - EX28DRAFT_3660 0.30 -0.2 327,933 - EX28DRAFT_3660 0.30 -0.6 327,933 - EX28DRAFT_3660 0.30 -0.4 327,933 - EX28DRAFT_3660 0.30 -0.3 327,933 - EX28DRAFT_3660 0.30 -0.8 327,933 - EX28DRAFT_3660 0.30 -2.2 327,933 - EX28DRAFT_3660 0.30 +0.0 327,933 - EX28DRAFT_3660 0.30 -0.5 327,936 + EX28DRAFT_3660 0.30 -0.1 327,936 + EX28DRAFT_3660 0.30 -0.9 327,937 - EX28DRAFT_3660 0.30 +0.3 327,937 - EX28DRAFT_3660 0.30 -1.5 327,937 - EX28DRAFT_3660 0.30 -0.1 327,978 + EX28DRAFT_3660 0.34 -1.0 327,978 + EX28DRAFT_3660 0.34 -0.2 327,979 - EX28DRAFT_3660 0.34 -0.2 327,981 - EX28DRAFT_3660 0.35 -0.5 327,981 - EX28DRAFT_3660 0.35 -0.5 327,981 - EX28DRAFT_3660 0.35 +0.5 328,238 + EX28DRAFT_3660 0.61 -0.6 328,253 + EX28DRAFT_3660 0.62 -0.1 328,545 - -1.3 328,545 - -0.5 328,550 + -0.2
Or see this region's nucleotide sequence