Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2893 and EX28DRAFT_2894 are separated by 34 nucleotides EX28DRAFT_2894 and EX28DRAFT_2895 overlap by 4 nucleotides
EX28DRAFT_2893: EX28DRAFT_2893 - N-acetylglutamate synthase (EC 2.3.1.1), at 197,070 to 198,401
_2893
EX28DRAFT_2894: EX28DRAFT_2894 - DNA helicase/exodeoxyribonuclease V, alpha subunit (EC 3.1.11.5), at 198,436 to 200,256
_2894
EX28DRAFT_2895: EX28DRAFT_2895 - DNA helicase/exodeoxyribonuclease V, beta subunit (EC 3.1.11.5), at 200,253 to 203,795
_2895
Position (kb) 198
199
200
201 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 197.549 kb on + strand, within EX28DRAFT_2893 at 197.549 kb on + strand, within EX28DRAFT_2893 at 197.550 kb on - strand, within EX28DRAFT_2893 at 197.877 kb on + strand, within EX28DRAFT_2893 at 198.189 kb on - strand, within EX28DRAFT_2893 at 198.354 kb on + strand at 198.354 kb on + strand at 198.354 kb on + strand at 198.355 kb on - strand at 198.355 kb on - strand at 198.355 kb on - strand at 198.360 kb on + strand at 198.360 kb on + strand at 198.361 kb on - strand at 198.361 kb on - strand at 198.361 kb on - strand at 198.560 kb on + strand at 198.560 kb on + strand at 198.560 kb on + strand at 198.561 kb on - strand at 198.580 kb on + strand at 198.580 kb on + strand at 198.580 kb on + strand at 198.580 kb on + strand at 198.642 kb on + strand, within EX28DRAFT_2894 at 198.643 kb on - strand, within EX28DRAFT_2894 at 198.948 kb on - strand, within EX28DRAFT_2894 at 198.982 kb on - strand, within EX28DRAFT_2894 at 198.995 kb on - strand, within EX28DRAFT_2894 at 198.995 kb on - strand, within EX28DRAFT_2894 at 199.049 kb on + strand, within EX28DRAFT_2894 at 199.084 kb on - strand, within EX28DRAFT_2894 at 199.313 kb on + strand, within EX28DRAFT_2894 at 199.937 kb on + strand, within EX28DRAFT_2894 at 199.937 kb on + strand, within EX28DRAFT_2894 at 199.937 kb on + strand, within EX28DRAFT_2894 at 199.939 kb on + strand, within EX28DRAFT_2894 at 199.939 kb on + strand, within EX28DRAFT_2894 at 200.008 kb on + strand, within EX28DRAFT_2894 at 200.008 kb on + strand, within EX28DRAFT_2894 at 200.009 kb on - strand, within EX28DRAFT_2894 at 200.107 kb on + strand at 200.107 kb on + strand at 200.107 kb on + strand at 200.107 kb on + strand at 200.107 kb on + strand at 200.107 kb on + strand at 200.107 kb on + strand at 200.108 kb on - strand at 200.108 kb on - strand at 200.108 kb on - strand at 200.108 kb on - strand at 200.108 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 197,549 + EX28DRAFT_2893 0.36 -3.4 197,549 + EX28DRAFT_2893 0.36 -2.3 197,550 - EX28DRAFT_2893 0.36 -3.8 197,877 + EX28DRAFT_2893 0.61 -1.9 198,189 - EX28DRAFT_2893 0.84 +0.5 198,354 + -2.6 198,354 + -3.5 198,354 + -2.0 198,355 - +0.2 198,355 - -2.9 198,355 - -2.2 198,360 + -2.2 198,360 + -0.4 198,361 - -1.2 198,361 - -0.9 198,361 - -0.2 198,560 + +1.1 198,560 + +0.5 198,560 + +0.5 198,561 - -0.7 198,580 + -0.0 198,580 + -0.3 198,580 + -0.1 198,580 + +0.0 198,642 + EX28DRAFT_2894 0.11 -0.3 198,643 - EX28DRAFT_2894 0.11 +0.3 198,948 - EX28DRAFT_2894 0.28 -0.3 198,982 - EX28DRAFT_2894 0.30 +1.4 198,995 - EX28DRAFT_2894 0.31 +0.4 198,995 - EX28DRAFT_2894 0.31 -0.3 199,049 + EX28DRAFT_2894 0.34 +1.2 199,084 - EX28DRAFT_2894 0.36 +0.2 199,313 + EX28DRAFT_2894 0.48 -0.1 199,937 + EX28DRAFT_2894 0.82 +0.4 199,937 + EX28DRAFT_2894 0.82 -0.2 199,937 + EX28DRAFT_2894 0.82 -0.4 199,939 + EX28DRAFT_2894 0.83 +0.8 199,939 + EX28DRAFT_2894 0.83 +1.0 200,008 + EX28DRAFT_2894 0.86 +0.7 200,008 + EX28DRAFT_2894 0.86 +0.1 200,009 - EX28DRAFT_2894 0.86 +0.4 200,107 + +0.1 200,107 + -0.7 200,107 + +2.5 200,107 + +0.1 200,107 + -0.4 200,107 + +0.3 200,107 + -0.0 200,108 - +0.2 200,108 - -0.2 200,108 - -0.1 200,108 - -0.0 200,108 - +0.0
Or see this region's nucleotide sequence