Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2213 and EX28DRAFT_2214 overlap by 23 nucleotides EX28DRAFT_2214 and EX28DRAFT_2215 overlap by 17 nucleotides EX28DRAFT_2215 and EX28DRAFT_2216 overlap by 4 nucleotides
EX28DRAFT_2213: EX28DRAFT_2213 - dethiobiotin synthase, at 2,236,572 to 2,237,288
_2213
EX28DRAFT_2214: EX28DRAFT_2214 - malonyl-acyl carrier protein O-methyltransferase BioC, at 2,237,266 to 2,238,021
_2214
EX28DRAFT_2215: EX28DRAFT_2215 - 8-amino-7-oxononanoate synthase, at 2,238,005 to 2,239,162
_2215
EX28DRAFT_2216: EX28DRAFT_2216 - biotin synthase (EC 2.8.1.6), at 2,239,159 to 2,240,199
_2216
Position (kb)
2238
2239
2240 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2237.163 kb on - strand, within EX28DRAFT_2213 at 2237.163 kb on - strand, within EX28DRAFT_2213 at 2237.260 kb on + strand at 2237.524 kb on + strand, within EX28DRAFT_2214 at 2237.963 kb on + strand at 2237.964 kb on - strand at 2237.964 kb on - strand at 2238.268 kb on + strand, within EX28DRAFT_2215 at 2238.268 kb on + strand, within EX28DRAFT_2215 at 2238.269 kb on - strand, within EX28DRAFT_2215 at 2238.269 kb on - strand, within EX28DRAFT_2215 at 2238.373 kb on - strand, within EX28DRAFT_2215 at 2238.919 kb on - strand, within EX28DRAFT_2215 at 2239.141 kb on + strand at 2239.141 kb on + strand at 2239.142 kb on - strand at 2239.344 kb on - strand, within EX28DRAFT_2216 at 2239.751 kb on + strand, within EX28DRAFT_2216 at 2239.751 kb on + strand, within EX28DRAFT_2216 at 2239.751 kb on + strand, within EX28DRAFT_2216 at 2239.752 kb on - strand, within EX28DRAFT_2216 at 2239.752 kb on - strand, within EX28DRAFT_2216 at 2239.755 kb on - strand, within EX28DRAFT_2216 at 2239.815 kb on + strand, within EX28DRAFT_2216 at 2239.816 kb on - strand, within EX28DRAFT_2216 at 2239.816 kb on - strand, within EX28DRAFT_2216 at 2239.865 kb on + strand, within EX28DRAFT_2216 at 2240.024 kb on + strand, within EX28DRAFT_2216
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,237,163 - EX28DRAFT_2213 0.82 -1.0 2,237,163 - EX28DRAFT_2213 0.82 -3.1 2,237,260 + -1.5 2,237,524 + EX28DRAFT_2214 0.34 -0.9 2,237,963 + -1.3 2,237,964 - +0.4 2,237,964 - +0.2 2,238,268 + EX28DRAFT_2215 0.23 -1.8 2,238,268 + EX28DRAFT_2215 0.23 -1.8 2,238,269 - EX28DRAFT_2215 0.23 -0.1 2,238,269 - EX28DRAFT_2215 0.23 -0.9 2,238,373 - EX28DRAFT_2215 0.32 -0.1 2,238,919 - EX28DRAFT_2215 0.79 -1.2 2,239,141 + -3.1 2,239,141 + -0.7 2,239,142 - +0.0 2,239,344 - EX28DRAFT_2216 0.18 -5.1 2,239,751 + EX28DRAFT_2216 0.57 -2.0 2,239,751 + EX28DRAFT_2216 0.57 -3.3 2,239,751 + EX28DRAFT_2216 0.57 -2.2 2,239,752 - EX28DRAFT_2216 0.57 -0.4 2,239,752 - EX28DRAFT_2216 0.57 -1.7 2,239,755 - EX28DRAFT_2216 0.57 -2.4 2,239,815 + EX28DRAFT_2216 0.63 -1.1 2,239,816 - EX28DRAFT_2216 0.63 -2.5 2,239,816 - EX28DRAFT_2216 0.63 -2.7 2,239,865 + EX28DRAFT_2216 0.68 -1.1 2,240,024 + EX28DRAFT_2216 0.83 -4.0
Or see this region's nucleotide sequence