Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0451 and EX28DRAFT_0452 are separated by 67 nucleotides EX28DRAFT_0452 and EX28DRAFT_0453 are separated by 159 nucleotides EX28DRAFT_0453 and EX28DRAFT_0454 are separated by 10 nucleotides
EX28DRAFT_0451: EX28DRAFT_0451 - sucrose-6-phosphate hydrolase, at 434,100 to 435,533
_0451
EX28DRAFT_0452: EX28DRAFT_0452 - hypothetical protein, at 435,601 to 436,071
_0452
EX28DRAFT_0453: EX28DRAFT_0453 - YadA-like C-terminal region/Collagen triple helix repeat (20 copies), at 436,231 to 437,082
_0453
EX28DRAFT_0454: EX28DRAFT_0454 - hypothetical protein, at 437,093 to 437,314
_0454
Position (kb)
436
437
438 Strain fitness (log2 ratio)
-2
-1
0
1 at 435.232 kb on - strand, within EX28DRAFT_0451 at 435.235 kb on + strand, within EX28DRAFT_0451 at 435.236 kb on + strand, within EX28DRAFT_0451 at 435.264 kb on + strand, within EX28DRAFT_0451 at 435.265 kb on - strand, within EX28DRAFT_0451 at 435.527 kb on - strand at 435.540 kb on + strand at 435.657 kb on + strand, within EX28DRAFT_0452 at 435.685 kb on - strand, within EX28DRAFT_0452 at 435.757 kb on + strand, within EX28DRAFT_0452 at 435.758 kb on - strand, within EX28DRAFT_0452 at 435.760 kb on + strand, within EX28DRAFT_0452 at 435.775 kb on - strand, within EX28DRAFT_0452 at 435.778 kb on - strand, within EX28DRAFT_0452 at 435.816 kb on + strand, within EX28DRAFT_0452 at 435.921 kb on - strand, within EX28DRAFT_0452 at 435.921 kb on - strand, within EX28DRAFT_0452 at 435.951 kb on - strand, within EX28DRAFT_0452 at 435.958 kb on - strand, within EX28DRAFT_0452 at 436.085 kb on + strand at 436.090 kb on - strand at 436.111 kb on + strand at 436.115 kb on + strand at 436.129 kb on - strand at 436.131 kb on - strand at 436.142 kb on + strand at 436.146 kb on + strand at 436.147 kb on - strand at 436.171 kb on - strand at 436.174 kb on - strand at 436.208 kb on + strand at 436.309 kb on - strand at 436.309 kb on - strand at 436.361 kb on + strand, within EX28DRAFT_0453 at 436.361 kb on + strand, within EX28DRAFT_0453 at 436.414 kb on + strand, within EX28DRAFT_0453 at 436.464 kb on + strand, within EX28DRAFT_0453 at 436.467 kb on + strand, within EX28DRAFT_0453 at 436.507 kb on - strand, within EX28DRAFT_0453 at 436.515 kb on + strand, within EX28DRAFT_0453 at 436.529 kb on - strand, within EX28DRAFT_0453 at 436.584 kb on + strand, within EX28DRAFT_0453 at 436.584 kb on + strand, within EX28DRAFT_0453 at 436.597 kb on - strand, within EX28DRAFT_0453 at 436.785 kb on + strand, within EX28DRAFT_0453 at 436.831 kb on + strand, within EX28DRAFT_0453 at 436.831 kb on + strand, within EX28DRAFT_0453 at 436.832 kb on - strand, within EX28DRAFT_0453 at 436.832 kb on - strand, within EX28DRAFT_0453 at 436.900 kb on - strand, within EX28DRAFT_0453 at 436.900 kb on - strand, within EX28DRAFT_0453 at 436.914 kb on + strand, within EX28DRAFT_0453 at 436.984 kb on - strand, within EX28DRAFT_0453 at 437.058 kb on + strand at 437.108 kb on - strand at 437.167 kb on + strand, within EX28DRAFT_0454 at 437.192 kb on - strand, within EX28DRAFT_0454 at 437.203 kb on + strand, within EX28DRAFT_0454 at 437.306 kb on - strand at 437.786 kb on + strand at 437.806 kb on + strand at 437.948 kb on - strand at 438.024 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 435,232 - EX28DRAFT_0451 0.79 -0.1 435,235 + EX28DRAFT_0451 0.79 -1.0 435,236 + EX28DRAFT_0451 0.79 -0.2 435,264 + EX28DRAFT_0451 0.81 +0.6 435,265 - EX28DRAFT_0451 0.81 -0.1 435,527 - +0.5 435,540 + +0.4 435,657 + EX28DRAFT_0452 0.12 -0.2 435,685 - EX28DRAFT_0452 0.18 +0.1 435,757 + EX28DRAFT_0452 0.33 +0.2 435,758 - EX28DRAFT_0452 0.33 -0.7 435,760 + EX28DRAFT_0452 0.34 -1.9 435,775 - EX28DRAFT_0452 0.37 -1.6 435,778 - EX28DRAFT_0452 0.38 -0.2 435,816 + EX28DRAFT_0452 0.46 -1.8 435,921 - EX28DRAFT_0452 0.68 +0.2 435,921 - EX28DRAFT_0452 0.68 +0.3 435,951 - EX28DRAFT_0452 0.74 +0.7 435,958 - EX28DRAFT_0452 0.76 +0.0 436,085 + -1.1 436,090 - +0.8 436,111 + -0.3 436,115 + +0.8 436,129 - -2.0 436,131 - +0.2 436,142 + -0.0 436,146 + -1.1 436,147 - -0.3 436,171 - -0.4 436,174 - +0.3 436,208 + +0.2 436,309 - -0.4 436,309 - -0.5 436,361 + EX28DRAFT_0453 0.15 +0.4 436,361 + EX28DRAFT_0453 0.15 +0.2 436,414 + EX28DRAFT_0453 0.21 +1.1 436,464 + EX28DRAFT_0453 0.27 +0.7 436,467 + EX28DRAFT_0453 0.28 +0.6 436,507 - EX28DRAFT_0453 0.32 -0.4 436,515 + EX28DRAFT_0453 0.33 +0.6 436,529 - EX28DRAFT_0453 0.35 -0.4 436,584 + EX28DRAFT_0453 0.41 +0.1 436,584 + EX28DRAFT_0453 0.41 +0.0 436,597 - EX28DRAFT_0453 0.43 +0.0 436,785 + EX28DRAFT_0453 0.65 +0.3 436,831 + EX28DRAFT_0453 0.70 -0.3 436,831 + EX28DRAFT_0453 0.70 -0.2 436,832 - EX28DRAFT_0453 0.71 +0.1 436,832 - EX28DRAFT_0453 0.71 -0.2 436,900 - EX28DRAFT_0453 0.79 +0.8 436,900 - EX28DRAFT_0453 0.79 -0.1 436,914 + EX28DRAFT_0453 0.80 -1.2 436,984 - EX28DRAFT_0453 0.88 +0.8 437,058 + -0.5 437,108 - +0.8 437,167 + EX28DRAFT_0454 0.33 -0.4 437,192 - EX28DRAFT_0454 0.45 -0.1 437,203 + EX28DRAFT_0454 0.50 -0.5 437,306 - -0.5 437,786 + +0.3 437,806 + +0.3 437,948 - -0.3 438,024 + +0.1
Or see this region's nucleotide sequence