Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0259 and EX28DRAFT_0260 overlap by 37 nucleotides EX28DRAFT_0260 and EX28DRAFT_0261 overlap by 23 nucleotides EX28DRAFT_0261 and EX28DRAFT_0262 overlap by 1 nucleotides EX28DRAFT_0262 and EX28DRAFT_0263 are separated by 14 nucleotides
EX28DRAFT_0259: EX28DRAFT_0259 - type VI secretion protein, VC_A0110 family, at 221,100 to 222,854
_0259
EX28DRAFT_0260: EX28DRAFT_0260 - type VI secretion protein, VC_A0111 family, at 222,818 to 223,903
_0260
EX28DRAFT_0261: EX28DRAFT_0261 - type VI secretion lipoprotein, VC_A0113 family, at 223,881 to 224,426
_0261
EX28DRAFT_0262: EX28DRAFT_0262 - type VI secretion system lysozyme-like protein, at 224,426 to 224,881
_0262
EX28DRAFT_0263: EX28DRAFT_0263 - hypothetical protein, at 224,896 to 225,345
_0263
Position (kb)
222
223
224 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 221.872 kb on + strand, within EX28DRAFT_0259 at 222.006 kb on - strand, within EX28DRAFT_0259 at 222.006 kb on - strand, within EX28DRAFT_0259 at 222.006 kb on - strand, within EX28DRAFT_0259 at 222.010 kb on + strand, within EX28DRAFT_0259 at 222.011 kb on - strand, within EX28DRAFT_0259 at 222.011 kb on - strand, within EX28DRAFT_0259 at 222.011 kb on - strand, within EX28DRAFT_0259 at 222.144 kb on + strand, within EX28DRAFT_0259 at 222.144 kb on + strand, within EX28DRAFT_0259 at 222.207 kb on + strand, within EX28DRAFT_0259 at 222.207 kb on + strand, within EX28DRAFT_0259 at 222.208 kb on - strand, within EX28DRAFT_0259 at 222.210 kb on + strand, within EX28DRAFT_0259 at 222.252 kb on - strand, within EX28DRAFT_0259 at 222.368 kb on + strand, within EX28DRAFT_0259 at 222.617 kb on + strand, within EX28DRAFT_0259 at 222.617 kb on + strand, within EX28DRAFT_0259 at 222.646 kb on - strand, within EX28DRAFT_0259 at 222.765 kb on + strand at 222.766 kb on - strand at 222.766 kb on - strand at 222.786 kb on - strand at 222.900 kb on + strand at 222.901 kb on - strand at 222.901 kb on - strand at 223.035 kb on - strand, within EX28DRAFT_0260 at 223.082 kb on - strand, within EX28DRAFT_0260 at 223.091 kb on + strand, within EX28DRAFT_0260 at 223.092 kb on - strand, within EX28DRAFT_0260 at 223.121 kb on + strand, within EX28DRAFT_0260 at 223.225 kb on + strand, within EX28DRAFT_0260 at 223.229 kb on + strand, within EX28DRAFT_0260 at 223.229 kb on + strand, within EX28DRAFT_0260 at 223.229 kb on + strand, within EX28DRAFT_0260 at 223.230 kb on - strand, within EX28DRAFT_0260 at 223.528 kb on + strand, within EX28DRAFT_0260 at 223.634 kb on + strand, within EX28DRAFT_0260 at 223.635 kb on - strand, within EX28DRAFT_0260 at 223.635 kb on - strand, within EX28DRAFT_0260 at 223.664 kb on + strand, within EX28DRAFT_0260 at 223.692 kb on + strand, within EX28DRAFT_0260 at 223.820 kb on - strand at 223.839 kb on - strand at 223.898 kb on - strand at 223.938 kb on - strand, within EX28DRAFT_0261 at 224.078 kb on + strand, within EX28DRAFT_0261 at 224.152 kb on - strand, within EX28DRAFT_0261 at 224.249 kb on + strand, within EX28DRAFT_0261 at 224.440 kb on + strand at 224.440 kb on + strand at 224.441 kb on - strand at 224.441 kb on - strand at 224.441 kb on - strand at 224.441 kb on - strand at 224.497 kb on + strand, within EX28DRAFT_0262 at 224.545 kb on + strand, within EX28DRAFT_0262 at 224.709 kb on + strand, within EX28DRAFT_0262 at 224.710 kb on - strand, within EX28DRAFT_0262 at 224.754 kb on - strand, within EX28DRAFT_0262 at 224.809 kb on + strand, within EX28DRAFT_0262 at 224.875 kb on + strand at 224.875 kb on + strand at 224.878 kb on - strand at 224.880 kb on - strand at 224.880 kb on - strand at 224.889 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 221,872 + EX28DRAFT_0259 0.44 -1.0 222,006 - EX28DRAFT_0259 0.52 -0.5 222,006 - EX28DRAFT_0259 0.52 +0.2 222,006 - EX28DRAFT_0259 0.52 -0.1 222,010 + EX28DRAFT_0259 0.52 -0.0 222,011 - EX28DRAFT_0259 0.52 +0.6 222,011 - EX28DRAFT_0259 0.52 +0.0 222,011 - EX28DRAFT_0259 0.52 +0.8 222,144 + EX28DRAFT_0259 0.59 -0.7 222,144 + EX28DRAFT_0259 0.59 +1.2 222,207 + EX28DRAFT_0259 0.63 -0.0 222,207 + EX28DRAFT_0259 0.63 -0.2 222,208 - EX28DRAFT_0259 0.63 +0.2 222,210 + EX28DRAFT_0259 0.63 +0.2 222,252 - EX28DRAFT_0259 0.66 -0.2 222,368 + EX28DRAFT_0259 0.72 +0.2 222,617 + EX28DRAFT_0259 0.86 +0.0 222,617 + EX28DRAFT_0259 0.86 -0.7 222,646 - EX28DRAFT_0259 0.88 -0.1 222,765 + +0.4 222,766 - +0.5 222,766 - -1.3 222,786 - -0.1 222,900 + +0.3 222,901 - +0.2 222,901 - +3.0 223,035 - EX28DRAFT_0260 0.20 -0.5 223,082 - EX28DRAFT_0260 0.24 +0.1 223,091 + EX28DRAFT_0260 0.25 +0.5 223,092 - EX28DRAFT_0260 0.25 +0.2 223,121 + EX28DRAFT_0260 0.28 +0.0 223,225 + EX28DRAFT_0260 0.37 +1.7 223,229 + EX28DRAFT_0260 0.38 +0.6 223,229 + EX28DRAFT_0260 0.38 +0.5 223,229 + EX28DRAFT_0260 0.38 +0.7 223,230 - EX28DRAFT_0260 0.38 +0.2 223,528 + EX28DRAFT_0260 0.65 +0.8 223,634 + EX28DRAFT_0260 0.75 +0.2 223,635 - EX28DRAFT_0260 0.75 -1.3 223,635 - EX28DRAFT_0260 0.75 -0.1 223,664 + EX28DRAFT_0260 0.78 -0.3 223,692 + EX28DRAFT_0260 0.80 -0.5 223,820 - -0.2 223,839 - -0.4 223,898 - +0.1 223,938 - EX28DRAFT_0261 0.10 +0.2 224,078 + EX28DRAFT_0261 0.36 +0.5 224,152 - EX28DRAFT_0261 0.50 +0.3 224,249 + EX28DRAFT_0261 0.67 -0.2 224,440 + +0.9 224,440 + +0.2 224,441 - +0.9 224,441 - -0.0 224,441 - +0.3 224,441 - +0.2 224,497 + EX28DRAFT_0262 0.16 -0.5 224,545 + EX28DRAFT_0262 0.26 +0.7 224,709 + EX28DRAFT_0262 0.62 +0.8 224,710 - EX28DRAFT_0262 0.62 -2.2 224,754 - EX28DRAFT_0262 0.72 +0.5 224,809 + EX28DRAFT_0262 0.84 -0.2 224,875 + -0.1 224,875 + -0.1 224,878 - +0.0 224,880 - -0.2 224,880 - -0.1 224,889 + +0.0
Or see this region's nucleotide sequence