Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03781 and CCNA_03782 are separated by 183 nucleotides CCNA_03782 and CCNA_03783 overlap by 4 nucleotides CCNA_03783 and CCNA_03784 are separated by 57 nucleotides
CCNA_03781: CCNA_03781 - aconitate hydratase, at 3,947,320 to 3,950,007
_03781
CCNA_03782: CCNA_03782 - cytochrome c biogenesis ATP-binding export protein CcmA, at 3,950,191 to 3,950,805
_03782
CCNA_03783: CCNA_03783 - cytochrome c biogenesis ABC transporter permease component CcmB, at 3,950,802 to 3,951,467
_03783
CCNA_03784: CCNA_03784 - hypothetical protein, at 3,951,525 to 3,952,418
_03784
Position (kb)
3950
3951
3952 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3950.024 kb on - strand at 3950.034 kb on - strand at 3950.191 kb on - strand at 3950.322 kb on - strand, within CCNA_03782 at 3950.477 kb on + strand, within CCNA_03782 at 3950.482 kb on - strand, within CCNA_03782 at 3950.500 kb on + strand, within CCNA_03782 at 3950.609 kb on + strand, within CCNA_03782 at 3950.614 kb on + strand, within CCNA_03782 at 3950.622 kb on - strand, within CCNA_03782 at 3950.794 kb on - strand at 3950.806 kb on - strand at 3950.806 kb on - strand at 3950.838 kb on + strand at 3950.851 kb on - strand at 3950.868 kb on + strand at 3951.037 kb on + strand, within CCNA_03783 at 3951.333 kb on + strand, within CCNA_03783 at 3951.360 kb on + strand, within CCNA_03783 at 3951.461 kb on + strand at 3951.464 kb on - strand at 3951.501 kb on - strand at 3951.515 kb on - strand at 3951.546 kb on + strand at 3951.554 kb on - strand at 3951.554 kb on - strand at 3951.560 kb on - strand at 3951.565 kb on - strand at 3951.580 kb on + strand at 3951.588 kb on - strand at 3951.632 kb on + strand, within CCNA_03784 at 3951.652 kb on - strand, within CCNA_03784 at 3951.657 kb on + strand, within CCNA_03784 at 3951.707 kb on + strand, within CCNA_03784 at 3951.707 kb on + strand, within CCNA_03784 at 3951.721 kb on + strand, within CCNA_03784 at 3951.721 kb on + strand, within CCNA_03784 at 3951.729 kb on - strand, within CCNA_03784 at 3951.729 kb on - strand, within CCNA_03784 at 3951.729 kb on - strand, within CCNA_03784 at 3951.734 kb on - strand, within CCNA_03784 at 3951.734 kb on - strand, within CCNA_03784 at 3951.765 kb on - strand, within CCNA_03784 at 3951.769 kb on - strand, within CCNA_03784 at 3951.779 kb on - strand, within CCNA_03784 at 3951.799 kb on + strand, within CCNA_03784 at 3951.861 kb on + strand, within CCNA_03784 at 3951.880 kb on + strand, within CCNA_03784 at 3951.890 kb on - strand, within CCNA_03784 at 3951.975 kb on + strand, within CCNA_03784 at 3951.978 kb on - strand, within CCNA_03784 at 3951.982 kb on + strand, within CCNA_03784 at 3951.988 kb on - strand, within CCNA_03784 at 3952.031 kb on - strand, within CCNA_03784 at 3952.071 kb on + strand, within CCNA_03784 at 3952.072 kb on + strand, within CCNA_03784 at 3952.080 kb on - strand, within CCNA_03784 at 3952.104 kb on + strand, within CCNA_03784 at 3952.116 kb on + strand, within CCNA_03784 at 3952.116 kb on + strand, within CCNA_03784 at 3952.131 kb on + strand, within CCNA_03784 at 3952.164 kb on + strand, within CCNA_03784 at 3952.172 kb on - strand, within CCNA_03784 at 3952.189 kb on - strand, within CCNA_03784 at 3952.195 kb on + strand, within CCNA_03784 at 3952.208 kb on - strand, within CCNA_03784 at 3952.208 kb on - strand, within CCNA_03784 at 3952.208 kb on - strand, within CCNA_03784 at 3952.311 kb on - strand, within CCNA_03784 at 3952.386 kb on - strand at 3952.396 kb on - strand at 3952.404 kb on + strand at 3952.467 kb on + strand at 3952.467 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 3,950,024 - -1.0 3,950,034 - -0.4 3,950,191 - +0.4 3,950,322 - CCNA_03782 0.21 +0.2 3,950,477 + CCNA_03782 0.47 -0.0 3,950,482 - CCNA_03782 0.47 +0.5 3,950,500 + CCNA_03782 0.50 +0.5 3,950,609 + CCNA_03782 0.68 -0.5 3,950,614 + CCNA_03782 0.69 +1.1 3,950,622 - CCNA_03782 0.70 +0.5 3,950,794 - -1.3 3,950,806 - -0.7 3,950,806 - +0.5 3,950,838 + -1.5 3,950,851 - +0.5 3,950,868 + -0.0 3,951,037 + CCNA_03783 0.35 +0.6 3,951,333 + CCNA_03783 0.80 +0.5 3,951,360 + CCNA_03783 0.84 -1.5 3,951,461 + +0.3 3,951,464 - +0.7 3,951,501 - +0.4 3,951,515 - -0.6 3,951,546 + +1.3 3,951,554 - +0.4 3,951,554 - -0.2 3,951,560 - +0.1 3,951,565 - +0.7 3,951,580 + +0.1 3,951,588 - -0.1 3,951,632 + CCNA_03784 0.12 -0.1 3,951,652 - CCNA_03784 0.14 +0.2 3,951,657 + CCNA_03784 0.15 +0.7 3,951,707 + CCNA_03784 0.20 -0.6 3,951,707 + CCNA_03784 0.20 +1.3 3,951,721 + CCNA_03784 0.22 -0.5 3,951,721 + CCNA_03784 0.22 +0.3 3,951,729 - CCNA_03784 0.23 +0.4 3,951,729 - CCNA_03784 0.23 -0.2 3,951,729 - CCNA_03784 0.23 +0.1 3,951,734 - CCNA_03784 0.23 +0.5 3,951,734 - CCNA_03784 0.23 +0.4 3,951,765 - CCNA_03784 0.27 +0.1 3,951,769 - CCNA_03784 0.27 +0.1 3,951,779 - CCNA_03784 0.28 +0.4 3,951,799 + CCNA_03784 0.31 -0.3 3,951,861 + CCNA_03784 0.38 +0.2 3,951,880 + CCNA_03784 0.40 -1.0 3,951,890 - CCNA_03784 0.41 -0.0 3,951,975 + CCNA_03784 0.50 +2.2 3,951,978 - CCNA_03784 0.51 -0.2 3,951,982 + CCNA_03784 0.51 +0.9 3,951,988 - CCNA_03784 0.52 +0.9 3,952,031 - CCNA_03784 0.57 -2.6 3,952,071 + CCNA_03784 0.61 +1.5 3,952,072 + CCNA_03784 0.61 +0.1 3,952,080 - CCNA_03784 0.62 +0.3 3,952,104 + CCNA_03784 0.65 -0.1 3,952,116 + CCNA_03784 0.66 +0.5 3,952,116 + CCNA_03784 0.66 +1.1 3,952,131 + CCNA_03784 0.68 -1.3 3,952,164 + CCNA_03784 0.71 -0.8 3,952,172 - CCNA_03784 0.72 +0.2 3,952,189 - CCNA_03784 0.74 +0.8 3,952,195 + CCNA_03784 0.75 +0.0 3,952,208 - CCNA_03784 0.76 -0.3 3,952,208 - CCNA_03784 0.76 +0.2 3,952,208 - CCNA_03784 0.76 -1.1 3,952,311 - CCNA_03784 0.88 -0.1 3,952,386 - +0.4 3,952,396 - +0.0 3,952,404 + -1.2 3,952,467 + -0.2 3,952,467 + -0.0
Or see this region's nucleotide sequence