Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03256 and CCNA_03990 are separated by 113 nucleotides CCNA_03990 and CCNA_03257 are separated by 27 nucleotides CCNA_03257 and CCNA_03258 are separated by 48 nucleotides CCNA_03258 and CCNA_03259 are separated by 11 nucleotides CCNA_03259 and CCNA_03260 are separated by 13 nucleotides CCNA_03260 and CCNA_03261 are separated by 72 nucleotides
CCNA_03256: CCNA_03256 - chorismate synthase, at 3,427,166 to 3,428,284
_03256
CCNA_03990: CCNA_03990 - hypothetical protein, at 3,428,398 to 3,428,430
_03990
CCNA_03257: CCNA_03257 - chemotaxis receiver domain protein CheYIII, at 3,428,458 to 3,429,000
_03257
CCNA_03258: CCNA_03258 - hypothetical protein, at 3,429,049 to 3,429,288
_03258
CCNA_03259: CCNA_03259 - mannose-6-phosphate isomerase, at 3,429,300 to 3,429,683
_03259
CCNA_03260: CCNA_03260 - queuosine biosynthesis protein QueD, at 3,429,697 to 3,430,062
_03260
CCNA_03261: CCNA_03261 - queuosine biosynthesis protein QueE, at 3,430,135 to 3,430,767
_03261
Position (kb)
3429
3430 Strain fitness (log2 ratio)
-2
-1
0
1 at 3428.202 kb on - strand at 3428.286 kb on - strand at 3428.296 kb on - strand at 3428.356 kb on + strand at 3428.361 kb on + strand at 3428.361 kb on + strand at 3428.372 kb on + strand at 3428.470 kb on + strand at 3428.551 kb on - strand, within CCNA_03257 at 3428.620 kb on - strand, within CCNA_03257 at 3428.629 kb on + strand, within CCNA_03257 at 3428.635 kb on - strand, within CCNA_03257 at 3428.675 kb on - strand, within CCNA_03257 at 3428.690 kb on + strand, within CCNA_03257 at 3428.698 kb on - strand, within CCNA_03257 at 3428.786 kb on - strand, within CCNA_03257 at 3428.798 kb on - strand, within CCNA_03257 at 3428.809 kb on + strand, within CCNA_03257 at 3428.819 kb on + strand, within CCNA_03257 at 3428.827 kb on - strand, within CCNA_03257 at 3428.925 kb on - strand, within CCNA_03257 at 3428.936 kb on + strand, within CCNA_03257 at 3428.969 kb on - strand at 3429.003 kb on - strand at 3429.003 kb on - strand at 3429.011 kb on + strand at 3429.025 kb on + strand at 3429.047 kb on + strand at 3429.047 kb on + strand at 3429.077 kb on + strand, within CCNA_03258 at 3429.077 kb on + strand, within CCNA_03258 at 3429.081 kb on + strand, within CCNA_03258 at 3429.089 kb on - strand, within CCNA_03258 at 3429.106 kb on - strand, within CCNA_03258 at 3429.142 kb on + strand, within CCNA_03258 at 3429.206 kb on + strand, within CCNA_03258 at 3429.326 kb on + strand at 3429.349 kb on - strand, within CCNA_03259 at 3429.373 kb on + strand, within CCNA_03259 at 3429.374 kb on + strand, within CCNA_03259 at 3429.374 kb on + strand, within CCNA_03259 at 3429.374 kb on + strand, within CCNA_03259 at 3429.374 kb on + strand, within CCNA_03259 at 3429.382 kb on - strand, within CCNA_03259 at 3429.382 kb on - strand, within CCNA_03259 at 3429.382 kb on - strand, within CCNA_03259 at 3429.605 kb on + strand, within CCNA_03259 at 3429.605 kb on + strand, within CCNA_03259 at 3429.605 kb on + strand, within CCNA_03259 at 3429.605 kb on + strand, within CCNA_03259 at 3429.605 kb on + strand, within CCNA_03259 at 3429.605 kb on + strand, within CCNA_03259 at 3429.605 kb on + strand, within CCNA_03259 at 3429.605 kb on + strand, within CCNA_03259 at 3429.605 kb on + strand, within CCNA_03259 at 3429.605 kb on + strand, within CCNA_03259 at 3429.605 kb on + strand, within CCNA_03259 at 3429.605 kb on + strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.613 kb on - strand, within CCNA_03259 at 3429.624 kb on + strand, within CCNA_03259 at 3429.626 kb on + strand, within CCNA_03259 at 3429.663 kb on + strand at 3429.681 kb on - strand at 3429.704 kb on - strand at 3429.756 kb on + strand, within CCNA_03260 at 3429.766 kb on - strand, within CCNA_03260 at 3429.794 kb on + strand, within CCNA_03260 at 3429.802 kb on - strand, within CCNA_03260 at 3429.812 kb on - strand, within CCNA_03260 at 3429.885 kb on + strand, within CCNA_03260 at 3429.893 kb on + strand, within CCNA_03260 at 3429.919 kb on + strand, within CCNA_03260 at 3429.939 kb on + strand, within CCNA_03260 at 3429.939 kb on + strand, within CCNA_03260 at 3429.951 kb on - strand, within CCNA_03260 at 3429.953 kb on - strand, within CCNA_03260 at 3430.034 kb on + strand at 3430.055 kb on + strand at 3430.055 kb on + strand at 3430.081 kb on + strand at 3430.091 kb on + strand at 3430.100 kb on - strand at 3430.100 kb on - strand at 3430.118 kb on - strand at 3430.204 kb on - strand, within CCNA_03261 at 3430.207 kb on + strand, within CCNA_03261 at 3430.230 kb on + strand, within CCNA_03261 at 3430.230 kb on + strand, within CCNA_03261 at 3430.248 kb on + strand, within CCNA_03261 at 3430.285 kb on + strand, within CCNA_03261
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 3,428,202 - -0.8 3,428,286 - +0.5 3,428,296 - -0.6 3,428,356 + +1.4 3,428,361 + +0.2 3,428,361 + +0.8 3,428,372 + +1.0 3,428,470 + +0.4 3,428,551 - CCNA_03257 0.17 +0.6 3,428,620 - CCNA_03257 0.30 -1.6 3,428,629 + CCNA_03257 0.31 -0.4 3,428,635 - CCNA_03257 0.33 +0.4 3,428,675 - CCNA_03257 0.40 -0.8 3,428,690 + CCNA_03257 0.43 -0.0 3,428,698 - CCNA_03257 0.44 -0.8 3,428,786 - CCNA_03257 0.60 -0.1 3,428,798 - CCNA_03257 0.63 +0.6 3,428,809 + CCNA_03257 0.65 +1.7 3,428,819 + CCNA_03257 0.66 +0.4 3,428,827 - CCNA_03257 0.68 -0.4 3,428,925 - CCNA_03257 0.86 -0.4 3,428,936 + CCNA_03257 0.88 +0.3 3,428,969 - +0.4 3,429,003 - -2.6 3,429,003 - -1.4 3,429,011 + +0.2 3,429,025 + +0.1 3,429,047 + +0.8 3,429,047 + -0.2 3,429,077 + CCNA_03258 0.12 -1.1 3,429,077 + CCNA_03258 0.12 +0.2 3,429,081 + CCNA_03258 0.13 +0.4 3,429,089 - CCNA_03258 0.17 +0.1 3,429,106 - CCNA_03258 0.24 -0.8 3,429,142 + CCNA_03258 0.39 -0.6 3,429,206 + CCNA_03258 0.65 +0.6 3,429,326 + +0.3 3,429,349 - CCNA_03259 0.13 -0.1 3,429,373 + CCNA_03259 0.19 +0.5 3,429,374 + CCNA_03259 0.19 -0.8 3,429,374 + CCNA_03259 0.19 +0.8 3,429,374 + CCNA_03259 0.19 +0.5 3,429,374 + CCNA_03259 0.19 -0.8 3,429,382 - CCNA_03259 0.21 +0.6 3,429,382 - CCNA_03259 0.21 +0.5 3,429,382 - CCNA_03259 0.21 +0.4 3,429,605 + CCNA_03259 0.79 -1.3 3,429,605 + CCNA_03259 0.79 -0.2 3,429,605 + CCNA_03259 0.79 -0.6 3,429,605 + CCNA_03259 0.79 -2.0 3,429,605 + CCNA_03259 0.79 -1.0 3,429,605 + CCNA_03259 0.79 -0.2 3,429,605 + CCNA_03259 0.79 -0.6 3,429,605 + CCNA_03259 0.79 +0.1 3,429,605 + CCNA_03259 0.79 +0.1 3,429,605 + CCNA_03259 0.79 -0.0 3,429,605 + CCNA_03259 0.79 +0.4 3,429,605 + CCNA_03259 0.79 +0.1 3,429,613 - CCNA_03259 0.82 +0.4 3,429,613 - CCNA_03259 0.82 +0.2 3,429,613 - CCNA_03259 0.82 +0.1 3,429,613 - CCNA_03259 0.82 -0.2 3,429,613 - CCNA_03259 0.82 -0.0 3,429,613 - CCNA_03259 0.82 +0.3 3,429,613 - CCNA_03259 0.82 -0.3 3,429,613 - CCNA_03259 0.82 +0.1 3,429,613 - CCNA_03259 0.82 -0.6 3,429,613 - CCNA_03259 0.82 +0.1 3,429,613 - CCNA_03259 0.82 +0.2 3,429,613 - CCNA_03259 0.82 +0.9 3,429,613 - CCNA_03259 0.82 +0.8 3,429,613 - CCNA_03259 0.82 +0.0 3,429,613 - CCNA_03259 0.82 -0.3 3,429,613 - CCNA_03259 0.82 +1.0 3,429,613 - CCNA_03259 0.82 +0.7 3,429,613 - CCNA_03259 0.82 +0.4 3,429,613 - CCNA_03259 0.82 +0.2 3,429,613 - CCNA_03259 0.82 -0.7 3,429,624 + CCNA_03259 0.84 +0.4 3,429,626 + CCNA_03259 0.85 +0.5 3,429,663 + +0.2 3,429,681 - +0.4 3,429,704 - +0.4 3,429,756 + CCNA_03260 0.16 -0.2 3,429,766 - CCNA_03260 0.19 +1.3 3,429,794 + CCNA_03260 0.27 -0.3 3,429,802 - CCNA_03260 0.29 -0.1 3,429,812 - CCNA_03260 0.31 +0.5 3,429,885 + CCNA_03260 0.51 -0.9 3,429,893 + CCNA_03260 0.54 -0.1 3,429,919 + CCNA_03260 0.61 -0.8 3,429,939 + CCNA_03260 0.66 -0.8 3,429,939 + CCNA_03260 0.66 -1.6 3,429,951 - CCNA_03260 0.69 +0.3 3,429,953 - CCNA_03260 0.70 -1.0 3,430,034 + -0.4 3,430,055 + +0.4 3,430,055 + +0.4 3,430,081 + -1.2 3,430,091 + -1.1 3,430,100 - +0.1 3,430,100 - +0.1 3,430,118 - +0.2 3,430,204 - CCNA_03261 0.11 -0.1 3,430,207 + CCNA_03261 0.11 -0.6 3,430,230 + CCNA_03261 0.15 -1.9 3,430,230 + CCNA_03261 0.15 -2.0 3,430,248 + CCNA_03261 0.18 -0.5 3,430,285 + CCNA_03261 0.24 -0.9
Or see this region's nucleotide sequence