Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02269 and CCNA_02270 are separated by 117 nucleotides CCNA_02270 and CCNA_02271 are separated by 227 nucleotides CCNA_02271 and CCNA_02272 are separated by 68 nucleotides CCNA_02272 and CCNA_02273 are separated by 34 nucleotides CCNA_02273 and CCNA_02274 are separated by 125 nucleotides
CCNA_02269: CCNA_02269 - L-aminopeptidase/D-esterase dmpA, at 2,421,986 to 2,422,978
_02269
CCNA_02270: CCNA_02270 - MarR-family transcriptional regulator, at 2,423,096 to 2,423,605
_02270
CCNA_02271: CCNA_02271 - stalk cross-band protein StpD, at 2,423,833 to 2,424,618
_02271
CCNA_02272: CCNA_02272 - hypothetical protein, at 2,424,687 to 2,424,929
_02272
CCNA_02273: CCNA_02273 - glutathione peroxidase, at 2,424,964 to 2,425,212
_02273
CCNA_02274: CCNA_02274 - EF-Hand domain protein, at 2,425,338 to 2,425,823
_02274
Position (kb)
2423
2424
2425 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2422.952 kb on + strand at 2422.960 kb on - strand at 2422.989 kb on - strand at 2423.086 kb on + strand at 2423.093 kb on + strand at 2423.094 kb on + strand at 2423.101 kb on - strand at 2423.102 kb on + strand at 2423.280 kb on - strand, within CCNA_02270 at 2423.325 kb on - strand, within CCNA_02270 at 2423.338 kb on + strand, within CCNA_02270 at 2423.391 kb on + strand, within CCNA_02270 at 2423.391 kb on + strand, within CCNA_02270 at 2423.425 kb on - strand, within CCNA_02270 at 2423.450 kb on + strand, within CCNA_02270 at 2423.458 kb on - strand, within CCNA_02270 at 2423.572 kb on + strand at 2423.580 kb on - strand at 2423.614 kb on + strand at 2423.662 kb on - strand at 2423.698 kb on - strand at 2423.723 kb on - strand at 2423.723 kb on - strand at 2423.754 kb on + strand at 2423.831 kb on + strand at 2424.008 kb on + strand, within CCNA_02271 at 2424.041 kb on + strand, within CCNA_02271 at 2424.041 kb on + strand, within CCNA_02271 at 2424.183 kb on - strand, within CCNA_02271 at 2424.229 kb on - strand, within CCNA_02271 at 2424.260 kb on + strand, within CCNA_02271 at 2424.262 kb on + strand, within CCNA_02271 at 2424.268 kb on - strand, within CCNA_02271 at 2424.269 kb on + strand, within CCNA_02271 at 2424.272 kb on + strand, within CCNA_02271 at 2424.280 kb on - strand, within CCNA_02271 at 2424.290 kb on - strand, within CCNA_02271 at 2424.290 kb on - strand, within CCNA_02271 at 2424.313 kb on + strand, within CCNA_02271 at 2424.370 kb on - strand, within CCNA_02271 at 2424.443 kb on + strand, within CCNA_02271 at 2424.577 kb on - strand at 2424.648 kb on + strand at 2424.747 kb on + strand, within CCNA_02272 at 2424.755 kb on - strand, within CCNA_02272 at 2424.805 kb on - strand, within CCNA_02272 at 2424.852 kb on + strand, within CCNA_02272 at 2424.904 kb on - strand, within CCNA_02272 at 2424.962 kb on + strand at 2425.006 kb on - strand, within CCNA_02273 at 2425.248 kb on + strand at 2425.343 kb on - strand at 2425.576 kb on + strand, within CCNA_02274 at 2425.576 kb on + strand, within CCNA_02274 at 2425.584 kb on - strand, within CCNA_02274 at 2425.584 kb on - strand, within CCNA_02274
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,422,952 + +0.0 2,422,960 - +0.6 2,422,989 - -0.8 2,423,086 + -2.0 2,423,093 + +0.3 2,423,094 + +0.6 2,423,101 - +0.6 2,423,102 + +0.3 2,423,280 - CCNA_02270 0.36 +2.9 2,423,325 - CCNA_02270 0.45 +0.1 2,423,338 + CCNA_02270 0.47 -0.2 2,423,391 + CCNA_02270 0.58 +0.6 2,423,391 + CCNA_02270 0.58 -0.4 2,423,425 - CCNA_02270 0.65 +0.0 2,423,450 + CCNA_02270 0.69 +1.0 2,423,458 - CCNA_02270 0.71 +0.2 2,423,572 + +0.1 2,423,580 - -0.1 2,423,614 + +0.6 2,423,662 - +0.0 2,423,698 - +0.5 2,423,723 - -0.3 2,423,723 - +0.3 2,423,754 + +0.2 2,423,831 + +1.8 2,424,008 + CCNA_02271 0.22 +0.3 2,424,041 + CCNA_02271 0.26 +0.5 2,424,041 + CCNA_02271 0.26 -0.1 2,424,183 - CCNA_02271 0.45 +0.6 2,424,229 - CCNA_02271 0.50 -1.1 2,424,260 + CCNA_02271 0.54 -1.8 2,424,262 + CCNA_02271 0.55 +0.2 2,424,268 - CCNA_02271 0.55 +0.2 2,424,269 + CCNA_02271 0.55 +0.5 2,424,272 + CCNA_02271 0.56 -0.1 2,424,280 - CCNA_02271 0.57 +0.0 2,424,290 - CCNA_02271 0.58 -0.4 2,424,290 - CCNA_02271 0.58 +0.5 2,424,313 + CCNA_02271 0.61 -1.3 2,424,370 - CCNA_02271 0.68 +0.3 2,424,443 + CCNA_02271 0.78 -1.3 2,424,577 - +0.5 2,424,648 + -0.6 2,424,747 + CCNA_02272 0.25 -0.8 2,424,755 - CCNA_02272 0.28 +0.4 2,424,805 - CCNA_02272 0.49 +0.7 2,424,852 + CCNA_02272 0.68 +0.0 2,424,904 - CCNA_02272 0.89 +1.7 2,424,962 + -1.6 2,425,006 - CCNA_02273 0.17 +1.2 2,425,248 + -0.5 2,425,343 - -0.2 2,425,576 + CCNA_02274 0.49 +0.1 2,425,576 + CCNA_02274 0.49 -0.7 2,425,584 - CCNA_02274 0.51 +0.6 2,425,584 - CCNA_02274 0.51 +0.3
Or see this region's nucleotide sequence