Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02194 and CCNA_02195 are separated by 7 nucleotides CCNA_02195 and CCNA_02196 are separated by 15 nucleotides CCNA_02196 and CCNA_02197 are separated by 88 nucleotides CCNA_02197 and CCNA_02198 are separated by 0 nucleotides
CCNA_02194: CCNA_02194 - fumarate hydratase, at 2,344,537 to 2,345,928
_02194
CCNA_02195: CCNA_02195 - acetyltransferase, at 2,345,936 to 2,346,370
_02195
CCNA_02196: CCNA_02196 - hypothetical protein, at 2,346,386 to 2,346,784
_02196
CCNA_02197: CCNA_02197 - hypothetical protein, at 2,346,873 to 2,347,463
_02197
CCNA_02198: CCNA_02198 - trans-isoprenyl diphosphate synthase, at 2,347,464 to 2,348,474
_02198
Position (kb)
2346
2347
2348 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2346.042 kb on - strand, within CCNA_02195 at 2346.184 kb on - strand, within CCNA_02195 at 2346.194 kb on - strand, within CCNA_02195 at 2346.230 kb on + strand, within CCNA_02195 at 2346.233 kb on - strand, within CCNA_02195 at 2346.233 kb on - strand, within CCNA_02195 at 2346.238 kb on - strand, within CCNA_02195 at 2346.253 kb on - strand, within CCNA_02195 at 2346.304 kb on + strand, within CCNA_02195 at 2346.314 kb on + strand, within CCNA_02195 at 2346.321 kb on + strand, within CCNA_02195 at 2346.329 kb on - strand at 2346.359 kb on + strand at 2346.365 kb on - strand at 2346.367 kb on + strand at 2346.408 kb on - strand at 2346.444 kb on - strand, within CCNA_02196 at 2346.512 kb on - strand, within CCNA_02196 at 2346.525 kb on - strand, within CCNA_02196 at 2346.553 kb on + strand, within CCNA_02196 at 2346.553 kb on + strand, within CCNA_02196 at 2346.563 kb on + strand, within CCNA_02196 at 2346.571 kb on - strand, within CCNA_02196 at 2346.571 kb on - strand, within CCNA_02196 at 2346.669 kb on + strand, within CCNA_02196 at 2346.669 kb on + strand, within CCNA_02196 at 2346.669 kb on + strand, within CCNA_02196 at 2346.669 kb on + strand, within CCNA_02196 at 2346.677 kb on - strand, within CCNA_02196 at 2346.677 kb on - strand, within CCNA_02196 at 2346.700 kb on + strand, within CCNA_02196 at 2346.721 kb on + strand, within CCNA_02196 at 2346.771 kb on - strand at 2346.800 kb on - strand at 2346.804 kb on + strand at 2346.804 kb on + strand at 2346.804 kb on + strand at 2346.804 kb on + strand at 2346.804 kb on + strand at 2346.812 kb on - strand at 2346.812 kb on - strand at 2346.859 kb on - strand at 2346.915 kb on - strand at 2346.988 kb on + strand, within CCNA_02197 at 2346.989 kb on - strand, within CCNA_02197 at 2347.007 kb on - strand, within CCNA_02197 at 2347.041 kb on + strand, within CCNA_02197 at 2347.060 kb on - strand, within CCNA_02197 at 2347.075 kb on + strand, within CCNA_02197 at 2347.086 kb on + strand, within CCNA_02197 at 2347.101 kb on + strand, within CCNA_02197 at 2347.109 kb on - strand, within CCNA_02197 at 2347.109 kb on - strand, within CCNA_02197 at 2347.114 kb on - strand, within CCNA_02197 at 2347.134 kb on + strand, within CCNA_02197 at 2347.142 kb on - strand, within CCNA_02197 at 2347.142 kb on - strand, within CCNA_02197 at 2347.143 kb on - strand, within CCNA_02197 at 2347.187 kb on + strand, within CCNA_02197 at 2347.215 kb on + strand, within CCNA_02197 at 2347.244 kb on + strand, within CCNA_02197 at 2347.244 kb on + strand, within CCNA_02197 at 2347.290 kb on + strand, within CCNA_02197 at 2347.298 kb on - strand, within CCNA_02197 at 2347.298 kb on - strand, within CCNA_02197 at 2347.308 kb on - strand, within CCNA_02197 at 2347.390 kb on + strand, within CCNA_02197 at 2348.434 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,346,042 - CCNA_02195 0.24 +0.0 2,346,184 - CCNA_02195 0.57 +0.1 2,346,194 - CCNA_02195 0.59 -0.8 2,346,230 + CCNA_02195 0.68 -0.0 2,346,233 - CCNA_02195 0.68 +0.6 2,346,233 - CCNA_02195 0.68 +0.0 2,346,238 - CCNA_02195 0.69 +0.5 2,346,253 - CCNA_02195 0.73 -0.0 2,346,304 + CCNA_02195 0.85 +0.3 2,346,314 + CCNA_02195 0.87 +0.2 2,346,321 + CCNA_02195 0.89 +0.0 2,346,329 - -0.4 2,346,359 + -0.7 2,346,365 - +0.8 2,346,367 + -0.8 2,346,408 - -0.6 2,346,444 - CCNA_02196 0.15 +0.2 2,346,512 - CCNA_02196 0.32 +0.1 2,346,525 - CCNA_02196 0.35 -0.7 2,346,553 + CCNA_02196 0.42 -0.4 2,346,553 + CCNA_02196 0.42 -1.0 2,346,563 + CCNA_02196 0.44 -0.8 2,346,571 - CCNA_02196 0.46 +0.4 2,346,571 - CCNA_02196 0.46 -2.1 2,346,669 + CCNA_02196 0.71 +1.0 2,346,669 + CCNA_02196 0.71 +0.1 2,346,669 + CCNA_02196 0.71 -1.2 2,346,669 + CCNA_02196 0.71 +0.8 2,346,677 - CCNA_02196 0.73 -0.3 2,346,677 - CCNA_02196 0.73 +0.4 2,346,700 + CCNA_02196 0.79 -0.7 2,346,721 + CCNA_02196 0.84 -0.0 2,346,771 - +0.3 2,346,800 - +0.0 2,346,804 + +1.8 2,346,804 + -0.4 2,346,804 + -0.9 2,346,804 + -0.1 2,346,804 + +0.2 2,346,812 - -0.0 2,346,812 - +0.3 2,346,859 - +0.1 2,346,915 - +0.5 2,346,988 + CCNA_02197 0.19 +0.0 2,346,989 - CCNA_02197 0.20 -0.2 2,347,007 - CCNA_02197 0.23 -0.6 2,347,041 + CCNA_02197 0.28 +0.3 2,347,060 - CCNA_02197 0.32 +1.5 2,347,075 + CCNA_02197 0.34 -0.2 2,347,086 + CCNA_02197 0.36 +0.4 2,347,101 + CCNA_02197 0.39 -0.2 2,347,109 - CCNA_02197 0.40 -0.4 2,347,109 - CCNA_02197 0.40 +0.2 2,347,114 - CCNA_02197 0.41 -0.4 2,347,134 + CCNA_02197 0.44 -0.2 2,347,142 - CCNA_02197 0.46 +1.2 2,347,142 - CCNA_02197 0.46 -0.7 2,347,143 - CCNA_02197 0.46 +0.1 2,347,187 + CCNA_02197 0.53 -2.1 2,347,215 + CCNA_02197 0.58 -0.4 2,347,244 + CCNA_02197 0.63 -1.5 2,347,244 + CCNA_02197 0.63 -1.3 2,347,290 + CCNA_02197 0.71 -2.6 2,347,298 - CCNA_02197 0.72 +0.4 2,347,298 - CCNA_02197 0.72 +0.2 2,347,308 - CCNA_02197 0.74 -0.2 2,347,390 + CCNA_02197 0.87 +0.6 2,348,434 + +0.3
Or see this region's nucleotide sequence