Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02017 and CCNA_02018 are separated by 8 nucleotides CCNA_02018 and CCNA_02019 overlap by 4 nucleotides CCNA_02019 and CCNA_02020 are separated by 82 nucleotides CCNA_02020 and CCNA_03961 are separated by 10 nucleotides CCNA_03961 and CCNA_02021 overlap by 4 nucleotides
CCNA_02017: CCNA_02017 - NADH-quinone oxidoreductase chain L, at 2,164,265 to 2,166,325
_02017
CCNA_02018: CCNA_02018 - NADH-quinone oxidoreductase chain K, at 2,166,334 to 2,166,639
_02018
CCNA_02019: CCNA_02019 - NADH-quinone oxidoreductase chain J, at 2,166,636 to 2,167,253
_02019
CCNA_02020: CCNA_02020 - NADH-quinone oxidoreductase chain I, at 2,167,336 to 2,167,827
_02020
CCNA_03961: CCNA_03961 - hypothetical protein, at 2,167,838 to 2,168,062
_03961
CCNA_02021: CCNA_02021 - hypothetical protein, at 2,168,059 to 2,168,298
_02021
Position (kb)
2166
2167
2168 Strain fitness (log2 ratio)
-1
0
1
2 at 2166.682 kb on - strand at 2166.682 kb on - strand at 2167.877 kb on - strand, within CCNA_03961 at 2167.877 kb on - strand, within CCNA_03961 at 2167.946 kb on - strand, within CCNA_03961 at 2167.946 kb on - strand, within CCNA_03961 at 2167.946 kb on - strand, within CCNA_03961 at 2167.959 kb on - strand, within CCNA_03961 at 2168.050 kb on - strand at 2168.063 kb on + strand at 2168.071 kb on - strand at 2168.108 kb on + strand, within CCNA_02021 at 2168.111 kb on - strand, within CCNA_02021 at 2168.114 kb on + strand, within CCNA_02021 at 2168.122 kb on - strand, within CCNA_02021 at 2168.137 kb on - strand, within CCNA_02021 at 2168.150 kb on + strand, within CCNA_02021 at 2168.150 kb on - strand, within CCNA_02021 at 2168.158 kb on - strand, within CCNA_02021 at 2168.158 kb on - strand, within CCNA_02021 at 2168.218 kb on - strand, within CCNA_02021
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,166,682 - +0.1 2,166,682 - -0.5 2,167,877 - CCNA_03961 0.17 +0.3 2,167,877 - CCNA_03961 0.17 +0.3 2,167,946 - CCNA_03961 0.48 +0.8 2,167,946 - CCNA_03961 0.48 +0.1 2,167,946 - CCNA_03961 0.48 +0.7 2,167,959 - CCNA_03961 0.54 +1.7 2,168,050 - -0.4 2,168,063 + -0.3 2,168,071 - +0.7 2,168,108 + CCNA_02021 0.20 -1.7 2,168,111 - CCNA_02021 0.22 +1.4 2,168,114 + CCNA_02021 0.23 +2.0 2,168,122 - CCNA_02021 0.26 +0.6 2,168,137 - CCNA_02021 0.33 +0.7 2,168,150 + CCNA_02021 0.38 +0.7 2,168,150 - CCNA_02021 0.38 +1.1 2,168,158 - CCNA_02021 0.41 +0.9 2,168,158 - CCNA_02021 0.41 +0.8 2,168,218 - CCNA_02021 0.66 -0.3
Or see this region's nucleotide sequence