Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01926 and CCNA_01927 are separated by 136 nucleotides CCNA_01927 and CCNA_01928 overlap by 14 nucleotides CCNA_01928 and CCNA_01929 overlap by 4 nucleotides CCNA_01929 and CCNA_01930 are separated by 0 nucleotides
CCNA_01926: CCNA_01926 - GGDEF diguanylate cyclase DgcB, at 2,072,620 to 2,073,681
_01926
CCNA_01927: CCNA_01927 - enoyl-CoA hydratase, at 2,073,818 to 2,074,621
_01927
CCNA_01928: CCNA_01928 - conserved ACT domain protein, at 2,074,608 to 2,075,018
_01928
CCNA_01929: CCNA_01929 - hypothetical protein, at 2,075,015 to 2,075,419
_01929
CCNA_01930: CCNA_01930 - ADP-heptose LPS heptosyltransferase, at 2,075,420 to 2,076,391
_01930
Position (kb)
2073
2074
2075 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2072.861 kb on + strand, within CCNA_01926 at 2072.882 kb on - strand, within CCNA_01926 at 2072.906 kb on - strand, within CCNA_01926 at 2072.954 kb on - strand, within CCNA_01926 at 2073.000 kb on - strand, within CCNA_01926 at 2073.182 kb on - strand, within CCNA_01926 at 2073.204 kb on - strand, within CCNA_01926 at 2073.227 kb on - strand, within CCNA_01926 at 2073.257 kb on + strand, within CCNA_01926 at 2073.265 kb on - strand, within CCNA_01926 at 2073.275 kb on - strand, within CCNA_01926 at 2073.282 kb on + strand, within CCNA_01926 at 2073.290 kb on - strand, within CCNA_01926 at 2073.357 kb on - strand, within CCNA_01926 at 2073.357 kb on - strand, within CCNA_01926 at 2073.357 kb on - strand, within CCNA_01926 at 2073.403 kb on - strand, within CCNA_01926 at 2073.441 kb on + strand, within CCNA_01926 at 2073.496 kb on - strand, within CCNA_01926 at 2073.542 kb on - strand, within CCNA_01926 at 2073.651 kb on + strand at 2073.659 kb on - strand at 2073.699 kb on - strand at 2073.699 kb on - strand at 2073.777 kb on - strand at 2073.779 kb on + strand at 2073.811 kb on - strand at 2073.823 kb on - strand at 2073.845 kb on + strand at 2073.850 kb on + strand at 2073.871 kb on - strand at 2073.903 kb on + strand, within CCNA_01927 at 2073.911 kb on - strand, within CCNA_01927 at 2073.933 kb on - strand, within CCNA_01927 at 2073.934 kb on - strand, within CCNA_01927 at 2073.956 kb on - strand, within CCNA_01927 at 2073.972 kb on + strand, within CCNA_01927 at 2073.980 kb on - strand, within CCNA_01927 at 2073.980 kb on - strand, within CCNA_01927 at 2073.984 kb on - strand, within CCNA_01927 at 2074.148 kb on + strand, within CCNA_01927 at 2074.148 kb on + strand, within CCNA_01927 at 2074.148 kb on + strand, within CCNA_01927 at 2074.177 kb on - strand, within CCNA_01927 at 2074.232 kb on - strand, within CCNA_01927 at 2074.256 kb on - strand, within CCNA_01927 at 2074.257 kb on - strand, within CCNA_01927 at 2074.279 kb on - strand, within CCNA_01927 at 2074.436 kb on - strand, within CCNA_01927 at 2074.446 kb on - strand, within CCNA_01927 at 2074.446 kb on - strand, within CCNA_01927 at 2074.485 kb on - strand, within CCNA_01927 at 2074.485 kb on - strand, within CCNA_01927 at 2074.485 kb on - strand, within CCNA_01927 at 2074.486 kb on - strand, within CCNA_01927 at 2074.496 kb on - strand, within CCNA_01927 at 2074.513 kb on + strand, within CCNA_01927 at 2074.532 kb on + strand, within CCNA_01927 at 2074.586 kb on + strand at 2074.594 kb on - strand at 2074.604 kb on + strand at 2074.606 kb on + strand at 2074.614 kb on - strand at 2074.614 kb on - strand at 2074.632 kb on - strand at 2074.637 kb on + strand at 2074.679 kb on - strand, within CCNA_01928 at 2074.688 kb on - strand, within CCNA_01928 at 2074.698 kb on + strand, within CCNA_01928 at 2074.769 kb on - strand, within CCNA_01928 at 2074.784 kb on - strand, within CCNA_01928 at 2074.784 kb on - strand, within CCNA_01928 at 2074.803 kb on + strand, within CCNA_01928 at 2074.803 kb on + strand, within CCNA_01928 at 2074.803 kb on + strand, within CCNA_01928 at 2074.803 kb on + strand, within CCNA_01928 at 2074.811 kb on - strand, within CCNA_01928 at 2074.854 kb on + strand, within CCNA_01928 at 2074.863 kb on + strand, within CCNA_01928 at 2075.026 kb on - strand at 2075.228 kb on + strand, within CCNA_01929 at 2075.228 kb on + strand, within CCNA_01929 at 2075.261 kb on - strand, within CCNA_01929 at 2075.360 kb on - strand, within CCNA_01929 at 2075.394 kb on + strand at 2075.412 kb on - strand at 2075.461 kb on - strand at 2075.462 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,072,861 + CCNA_01926 0.23 +0.0 2,072,882 - CCNA_01926 0.25 -2.0 2,072,906 - CCNA_01926 0.27 +0.4 2,072,954 - CCNA_01926 0.31 -0.1 2,073,000 - CCNA_01926 0.36 -0.9 2,073,182 - CCNA_01926 0.53 +0.3 2,073,204 - CCNA_01926 0.55 -0.1 2,073,227 - CCNA_01926 0.57 -0.1 2,073,257 + CCNA_01926 0.60 +0.7 2,073,265 - CCNA_01926 0.61 +1.1 2,073,275 - CCNA_01926 0.62 +0.1 2,073,282 + CCNA_01926 0.62 +0.1 2,073,290 - CCNA_01926 0.63 -0.8 2,073,357 - CCNA_01926 0.69 +1.1 2,073,357 - CCNA_01926 0.69 -0.9 2,073,357 - CCNA_01926 0.69 -0.1 2,073,403 - CCNA_01926 0.74 -0.8 2,073,441 + CCNA_01926 0.77 -1.1 2,073,496 - CCNA_01926 0.82 +0.5 2,073,542 - CCNA_01926 0.87 +2.7 2,073,651 + -0.0 2,073,659 - -0.8 2,073,699 - -0.6 2,073,699 - -0.8 2,073,777 - +0.1 2,073,779 + +0.3 2,073,811 - -0.1 2,073,823 - +0.2 2,073,845 + +0.7 2,073,850 + -0.1 2,073,871 - +0.7 2,073,903 + CCNA_01927 0.11 +0.3 2,073,911 - CCNA_01927 0.12 -0.0 2,073,933 - CCNA_01927 0.14 -1.3 2,073,934 - CCNA_01927 0.14 -0.3 2,073,956 - CCNA_01927 0.17 +0.3 2,073,972 + CCNA_01927 0.19 +0.5 2,073,980 - CCNA_01927 0.20 +0.3 2,073,980 - CCNA_01927 0.20 +1.9 2,073,984 - CCNA_01927 0.21 -1.1 2,074,148 + CCNA_01927 0.41 +0.1 2,074,148 + CCNA_01927 0.41 -0.7 2,074,148 + CCNA_01927 0.41 +1.4 2,074,177 - CCNA_01927 0.45 +0.4 2,074,232 - CCNA_01927 0.51 -0.4 2,074,256 - CCNA_01927 0.54 -0.8 2,074,257 - CCNA_01927 0.55 -0.6 2,074,279 - CCNA_01927 0.57 -0.1 2,074,436 - CCNA_01927 0.77 +0.4 2,074,446 - CCNA_01927 0.78 -1.0 2,074,446 - CCNA_01927 0.78 +0.2 2,074,485 - CCNA_01927 0.83 -0.8 2,074,485 - CCNA_01927 0.83 -0.0 2,074,485 - CCNA_01927 0.83 -0.4 2,074,486 - CCNA_01927 0.83 +1.2 2,074,496 - CCNA_01927 0.84 -1.1 2,074,513 + CCNA_01927 0.86 -0.0 2,074,532 + CCNA_01927 0.89 +0.4 2,074,586 + +0.0 2,074,594 - +0.5 2,074,604 + +0.1 2,074,606 + +0.2 2,074,614 - +0.4 2,074,614 - +0.7 2,074,632 - +0.7 2,074,637 + -0.1 2,074,679 - CCNA_01928 0.17 -0.3 2,074,688 - CCNA_01928 0.19 +0.5 2,074,698 + CCNA_01928 0.22 -0.5 2,074,769 - CCNA_01928 0.39 -1.2 2,074,784 - CCNA_01928 0.43 +1.0 2,074,784 - CCNA_01928 0.43 +1.0 2,074,803 + CCNA_01928 0.47 +0.6 2,074,803 + CCNA_01928 0.47 -0.7 2,074,803 + CCNA_01928 0.47 +0.3 2,074,803 + CCNA_01928 0.47 +0.1 2,074,811 - CCNA_01928 0.49 +1.1 2,074,854 + CCNA_01928 0.60 -0.8 2,074,863 + CCNA_01928 0.62 -0.4 2,075,026 - +0.5 2,075,228 + CCNA_01929 0.53 +0.9 2,075,228 + CCNA_01929 0.53 +0.4 2,075,261 - CCNA_01929 0.61 +0.2 2,075,360 - CCNA_01929 0.85 +0.7 2,075,394 + -0.2 2,075,412 - -0.2 2,075,461 - +0.8 2,075,462 - +1.3
Or see this region's nucleotide sequence