Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01808 and CCNA_01809 are separated by 82 nucleotides CCNA_01809 and CCNA_01810 are separated by 3 nucleotides CCNA_01810 and CCNA_01811 overlap by 4 nucleotides CCNA_01811 and CCNA_01812 are separated by 38 nucleotides
CCNA_01808: CCNA_01808 - lysophospholipase L2, at 1,933,343 to 1,934,419
_01808
CCNA_01809: CCNA_01809 - lipoic acid synthetase, at 1,934,502 to 1,935,479
_01809
CCNA_01810: CCNA_01810 - coenzyme Q-binding protein, at 1,935,483 to 1,935,929
_01810
CCNA_01811: CCNA_01811 - competence/damage-inducible protein CinA, at 1,935,926 to 1,936,417
_01811
CCNA_01812: CCNA_01812 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 1,936,456 to 1,937,604
_01812
Position (kb)
1934
1935
1936 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1933.507 kb on - strand, within CCNA_01808 at 1933.521 kb on + strand, within CCNA_01808 at 1933.521 kb on + strand, within CCNA_01808 at 1933.528 kb on - strand, within CCNA_01808 at 1933.617 kb on - strand, within CCNA_01808 at 1933.647 kb on - strand, within CCNA_01808 at 1933.681 kb on + strand, within CCNA_01808 at 1933.692 kb on + strand, within CCNA_01808 at 1933.692 kb on + strand, within CCNA_01808 at 1933.692 kb on + strand, within CCNA_01808 at 1933.692 kb on + strand, within CCNA_01808 at 1933.699 kb on - strand, within CCNA_01808 at 1933.700 kb on - strand, within CCNA_01808 at 1933.700 kb on - strand, within CCNA_01808 at 1933.700 kb on - strand, within CCNA_01808 at 1933.742 kb on - strand, within CCNA_01808 at 1933.779 kb on - strand, within CCNA_01808 at 1933.803 kb on - strand, within CCNA_01808 at 1933.841 kb on + strand, within CCNA_01808 at 1933.850 kb on - strand, within CCNA_01808 at 1933.861 kb on + strand, within CCNA_01808 at 1933.880 kb on + strand, within CCNA_01808 at 1933.882 kb on + strand, within CCNA_01808 at 1933.947 kb on + strand, within CCNA_01808 at 1933.974 kb on + strand, within CCNA_01808 at 1933.982 kb on + strand, within CCNA_01808 at 1933.992 kb on + strand, within CCNA_01808 at 1934.000 kb on - strand, within CCNA_01808 at 1934.000 kb on - strand, within CCNA_01808 at 1934.000 kb on - strand, within CCNA_01808 at 1934.042 kb on - strand, within CCNA_01808 at 1934.058 kb on - strand, within CCNA_01808 at 1934.071 kb on + strand, within CCNA_01808 at 1934.079 kb on - strand, within CCNA_01808 at 1934.079 kb on - strand, within CCNA_01808 at 1934.191 kb on - strand, within CCNA_01808 at 1934.231 kb on + strand, within CCNA_01808 at 1934.231 kb on + strand, within CCNA_01808 at 1934.236 kb on + strand, within CCNA_01808 at 1934.236 kb on + strand, within CCNA_01808 at 1934.236 kb on + strand, within CCNA_01808 at 1934.244 kb on - strand, within CCNA_01808 at 1934.279 kb on - strand, within CCNA_01808 at 1934.286 kb on + strand, within CCNA_01808 at 1934.294 kb on - strand, within CCNA_01808 at 1934.301 kb on + strand, within CCNA_01808 at 1934.309 kb on - strand, within CCNA_01808 at 1934.322 kb on - strand at 1934.322 kb on - strand at 1934.322 kb on - strand at 1934.324 kb on - strand at 1934.376 kb on - strand at 1934.378 kb on - strand at 1934.397 kb on + strand at 1934.405 kb on - strand at 1935.437 kb on + strand at 1935.445 kb on - strand at 1935.473 kb on + strand at 1935.480 kb on + strand at 1935.488 kb on - strand at 1936.070 kb on - strand, within CCNA_01811 at 1936.123 kb on + strand, within CCNA_01811 at 1936.131 kb on + strand, within CCNA_01811 at 1936.138 kb on - strand, within CCNA_01811 at 1936.197 kb on - strand, within CCNA_01811 at 1936.228 kb on - strand, within CCNA_01811 at 1936.339 kb on - strand, within CCNA_01811 at 1936.445 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 1,933,507 - CCNA_01808 0.15 +1.4 1,933,521 + CCNA_01808 0.17 -0.4 1,933,521 + CCNA_01808 0.17 -0.2 1,933,528 - CCNA_01808 0.17 -0.0 1,933,617 - CCNA_01808 0.25 +0.7 1,933,647 - CCNA_01808 0.28 -0.2 1,933,681 + CCNA_01808 0.31 -0.4 1,933,692 + CCNA_01808 0.32 -1.9 1,933,692 + CCNA_01808 0.32 -3.3 1,933,692 + CCNA_01808 0.32 +0.2 1,933,692 + CCNA_01808 0.32 -0.5 1,933,699 - CCNA_01808 0.33 -0.8 1,933,700 - CCNA_01808 0.33 +0.0 1,933,700 - CCNA_01808 0.33 -1.4 1,933,700 - CCNA_01808 0.33 -0.9 1,933,742 - CCNA_01808 0.37 -0.0 1,933,779 - CCNA_01808 0.40 -0.2 1,933,803 - CCNA_01808 0.43 -0.6 1,933,841 + CCNA_01808 0.46 -1.6 1,933,850 - CCNA_01808 0.47 +1.8 1,933,861 + CCNA_01808 0.48 +0.6 1,933,880 + CCNA_01808 0.50 +1.4 1,933,882 + CCNA_01808 0.50 +0.8 1,933,947 + CCNA_01808 0.56 +1.4 1,933,974 + CCNA_01808 0.59 +1.0 1,933,982 + CCNA_01808 0.59 -0.2 1,933,992 + CCNA_01808 0.60 +1.3 1,934,000 - CCNA_01808 0.61 -0.8 1,934,000 - CCNA_01808 0.61 -0.9 1,934,000 - CCNA_01808 0.61 +0.3 1,934,042 - CCNA_01808 0.65 +0.4 1,934,058 - CCNA_01808 0.66 -0.3 1,934,071 + CCNA_01808 0.68 +0.7 1,934,079 - CCNA_01808 0.68 -0.6 1,934,079 - CCNA_01808 0.68 +0.2 1,934,191 - CCNA_01808 0.79 -2.6 1,934,231 + CCNA_01808 0.82 -0.5 1,934,231 + CCNA_01808 0.82 +0.1 1,934,236 + CCNA_01808 0.83 -0.4 1,934,236 + CCNA_01808 0.83 +1.7 1,934,236 + CCNA_01808 0.83 -0.6 1,934,244 - CCNA_01808 0.84 -1.1 1,934,279 - CCNA_01808 0.87 +0.6 1,934,286 + CCNA_01808 0.88 -0.1 1,934,294 - CCNA_01808 0.88 +0.3 1,934,301 + CCNA_01808 0.89 +0.1 1,934,309 - CCNA_01808 0.90 +0.5 1,934,322 - -0.2 1,934,322 - +0.2 1,934,322 - -1.2 1,934,324 - -0.9 1,934,376 - -2.4 1,934,378 - +0.7 1,934,397 + +0.2 1,934,405 - -0.8 1,935,437 + +0.1 1,935,445 - -2.4 1,935,473 + +1.8 1,935,480 + +0.4 1,935,488 - +0.6 1,936,070 - CCNA_01811 0.29 +0.5 1,936,123 + CCNA_01811 0.40 +0.5 1,936,131 + CCNA_01811 0.42 +0.2 1,936,138 - CCNA_01811 0.43 +0.9 1,936,197 - CCNA_01811 0.55 -1.0 1,936,228 - CCNA_01811 0.61 +0.5 1,936,339 - CCNA_01811 0.84 +0.5 1,936,445 + +0.6
Or see this region's nucleotide sequence