Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01750 and CCNA_01751 overlap by 4 nucleotides CCNA_01751 and CCNA_01752 are separated by 3 nucleotides CCNA_01752 and CCNA_01753 overlap by 8 nucleotides
CCNA_01750: CCNA_01750 - 3-oxoacyl-(acyl-carrier-protein) synthase II, at 1,877,432 to 1,878,712
_01750
CCNA_01751: CCNA_01751 - aminodeoxychorismate lyase, at 1,878,709 to 1,879,809
_01751
CCNA_01752: CCNA_01752 - YicC-family protein, at 1,879,813 to 1,880,694
_01752
CCNA_01753: CCNA_01753 - guanylate kinase, at 1,880,687 to 1,881,328
_01753
Position (kb) 1878
1879
1880 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1878.849 kb on + strand, within CCNA_01751 at 1879.019 kb on - strand, within CCNA_01751 at 1879.155 kb on + strand, within CCNA_01751 at 1879.156 kb on + strand, within CCNA_01751 at 1879.235 kb on - strand, within CCNA_01751 at 1879.243 kb on + strand, within CCNA_01751 at 1879.711 kb on - strand at 1879.713 kb on + strand at 1879.983 kb on + strand, within CCNA_01752 at 1879.991 kb on - strand, within CCNA_01752 at 1880.031 kb on + strand, within CCNA_01752 at 1880.143 kb on - strand, within CCNA_01752 at 1880.160 kb on - strand, within CCNA_01752 at 1880.181 kb on - strand, within CCNA_01752 at 1880.239 kb on - strand, within CCNA_01752 at 1880.254 kb on - strand, within CCNA_01752 at 1880.334 kb on + strand, within CCNA_01752 at 1880.342 kb on - strand, within CCNA_01752 at 1880.401 kb on - strand, within CCNA_01752 at 1880.479 kb on + strand, within CCNA_01752 at 1880.498 kb on + strand, within CCNA_01752 at 1880.506 kb on - strand, within CCNA_01752 at 1880.531 kb on - strand, within CCNA_01752 at 1880.635 kb on + strand at 1880.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 1,878,849 + CCNA_01751 0.13 +0.9 1,879,019 - CCNA_01751 0.28 +0.7 1,879,155 + CCNA_01751 0.41 +0.1 1,879,156 + CCNA_01751 0.41 +1.5 1,879,235 - CCNA_01751 0.48 -0.7 1,879,243 + CCNA_01751 0.49 +0.1 1,879,711 - +1.1 1,879,713 + +1.0 1,879,983 + CCNA_01752 0.19 +0.4 1,879,991 - CCNA_01752 0.20 -1.0 1,880,031 + CCNA_01752 0.25 +0.3 1,880,143 - CCNA_01752 0.37 +0.6 1,880,160 - CCNA_01752 0.39 +1.0 1,880,181 - CCNA_01752 0.42 +0.1 1,880,239 - CCNA_01752 0.48 +0.2 1,880,254 - CCNA_01752 0.50 -2.9 1,880,334 + CCNA_01752 0.59 +0.8 1,880,342 - CCNA_01752 0.60 +0.3 1,880,401 - CCNA_01752 0.67 -0.6 1,880,479 + CCNA_01752 0.76 +1.2 1,880,498 + CCNA_01752 0.78 +1.1 1,880,506 - CCNA_01752 0.79 +0.0 1,880,531 - CCNA_01752 0.81 -0.9 1,880,635 + +0.3 1,880,644 - +0.1
Or see this region's nucleotide sequence