Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01590 and CCNA_01591 overlap by 25 nucleotides CCNA_01591 and CCNA_01592 are separated by 115 nucleotides CCNA_01592 and CCNA_01593 are separated by 138 nucleotides
CCNA_01590: CCNA_01590 - NAD-dependent DNA ligase, at 1,709,381 to 1,711,732
_01590
CCNA_01591: CCNA_01591 - DNA polymerase III epsilon-like protein, at 1,711,708 to 1,712,337
_01591
CCNA_01592: CCNA_01592 - SN-glycerol-3-phosphate transport ATP-binding protein, at 1,712,453 to 1,712,881
_01592
CCNA_01593: CCNA_01593 - Xaa-pro aminopeptidase, at 1,713,020 to 1,714,831
_01593
Position (kb)
1712
1713 Strain fitness (log2 ratio)
-1
0
1 at 1711.760 kb on + strand at 1711.768 kb on - strand at 1711.781 kb on - strand, within CCNA_01591 at 1711.844 kb on - strand, within CCNA_01591 at 1711.870 kb on + strand, within CCNA_01591 at 1711.884 kb on + strand, within CCNA_01591 at 1711.890 kb on - strand, within CCNA_01591 at 1711.936 kb on - strand, within CCNA_01591 at 1711.996 kb on + strand, within CCNA_01591 at 1712.033 kb on + strand, within CCNA_01591 at 1712.039 kb on - strand, within CCNA_01591 at 1712.062 kb on - strand, within CCNA_01591 at 1712.165 kb on - strand, within CCNA_01591 at 1712.304 kb on - strand at 1712.339 kb on - strand at 1712.399 kb on - strand at 1712.422 kb on - strand at 1712.539 kb on + strand, within CCNA_01592 at 1712.548 kb on - strand, within CCNA_01592 at 1712.562 kb on - strand, within CCNA_01592 at 1712.611 kb on + strand, within CCNA_01592 at 1712.682 kb on + strand, within CCNA_01592 at 1712.800 kb on + strand, within CCNA_01592 at 1712.959 kb on + strand at 1712.967 kb on - strand at 1712.981 kb on + strand at 1712.990 kb on - strand at 1713.009 kb on - strand at 1713.058 kb on - strand at 1713.091 kb on + strand at 1713.091 kb on + strand at 1713.140 kb on - strand at 1713.165 kb on - strand at 1713.179 kb on + strand at 1713.187 kb on - strand at 1713.264 kb on + strand, within CCNA_01593 at 1713.387 kb on - strand, within CCNA_01593 at 1713.449 kb on - strand, within CCNA_01593 at 1713.525 kb on + strand, within CCNA_01593 at 1713.525 kb on + strand, within CCNA_01593 at 1713.546 kb on - strand, within CCNA_01593 at 1713.659 kb on - strand, within CCNA_01593 at 1713.800 kb on + strand, within CCNA_01593 at 1713.836 kb on + strand, within CCNA_01593 at 1713.836 kb on + strand, within CCNA_01593 at 1713.844 kb on - strand, within CCNA_01593 at 1713.844 kb on - strand, within CCNA_01593
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 1,711,760 + -0.1 1,711,768 - +0.6 1,711,781 - CCNA_01591 0.12 -0.4 1,711,844 - CCNA_01591 0.22 -1.6 1,711,870 + CCNA_01591 0.26 -0.1 1,711,884 + CCNA_01591 0.28 +1.5 1,711,890 - CCNA_01591 0.29 +0.8 1,711,936 - CCNA_01591 0.36 +0.3 1,711,996 + CCNA_01591 0.46 +0.3 1,712,033 + CCNA_01591 0.52 +0.3 1,712,039 - CCNA_01591 0.53 -0.7 1,712,062 - CCNA_01591 0.56 +0.2 1,712,165 - CCNA_01591 0.73 -1.1 1,712,304 - -1.2 1,712,339 - +1.4 1,712,399 - -0.0 1,712,422 - +1.2 1,712,539 + CCNA_01592 0.20 -0.4 1,712,548 - CCNA_01592 0.22 -1.4 1,712,562 - CCNA_01592 0.25 +0.4 1,712,611 + CCNA_01592 0.37 +0.5 1,712,682 + CCNA_01592 0.53 +0.1 1,712,800 + CCNA_01592 0.81 +0.7 1,712,959 + +0.3 1,712,967 - +0.5 1,712,981 + -0.0 1,712,990 - +0.1 1,713,009 - +0.3 1,713,058 - +1.0 1,713,091 + -0.4 1,713,091 + -0.1 1,713,140 - +1.2 1,713,165 - +0.4 1,713,179 + -0.4 1,713,187 - +0.2 1,713,264 + CCNA_01593 0.13 +0.6 1,713,387 - CCNA_01593 0.20 -0.4 1,713,449 - CCNA_01593 0.24 +0.3 1,713,525 + CCNA_01593 0.28 -0.5 1,713,525 + CCNA_01593 0.28 +0.3 1,713,546 - CCNA_01593 0.29 +1.1 1,713,659 - CCNA_01593 0.35 -0.0 1,713,800 + CCNA_01593 0.43 -1.0 1,713,836 + CCNA_01593 0.45 +0.1 1,713,836 + CCNA_01593 0.45 +0.5 1,713,844 - CCNA_01593 0.45 +1.2 1,713,844 - CCNA_01593 0.45 +1.1
Or see this region's nucleotide sequence