Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00361 and CCNA_00362 are separated by 51 nucleotides CCNA_00362 and CCNA_00363 are separated by 36 nucleotides CCNA_00363 and CCNA_00364 are separated by 78 nucleotides
CCNA_00361: CCNA_00361 - hot dog superfamily thioesterase, at 377,803 to 378,750
_00361
CCNA_00362: CCNA_00362 - zinc uptake regulation protein, at 378,802 to 379,287
_00362
CCNA_00363: CCNA_00363 - PAP2 bacterial acid phosphatase, at 379,324 to 380,100
_00363
CCNA_00364: CCNA_00364 - deoxyhypusine synthase, at 380,179 to 381,273
_00364
Position (kb)
379
380
381 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 378.325 kb on - strand, within CCNA_00361 at 378.361 kb on - strand, within CCNA_00361 at 378.401 kb on + strand, within CCNA_00361 at 378.453 kb on + strand, within CCNA_00361 at 378.468 kb on + strand, within CCNA_00361 at 378.499 kb on - strand, within CCNA_00361 at 378.511 kb on - strand, within CCNA_00361 at 378.520 kb on + strand, within CCNA_00361 at 378.562 kb on - strand, within CCNA_00361 at 378.562 kb on - strand, within CCNA_00361 at 378.562 kb on - strand, within CCNA_00361 at 378.577 kb on + strand, within CCNA_00361 at 378.633 kb on - strand, within CCNA_00361 at 378.648 kb on + strand, within CCNA_00361 at 378.658 kb on + strand at 378.698 kb on - strand at 378.764 kb on - strand at 378.833 kb on - strand at 378.855 kb on + strand, within CCNA_00362 at 378.855 kb on + strand, within CCNA_00362 at 378.862 kb on + strand, within CCNA_00362 at 378.893 kb on + strand, within CCNA_00362 at 378.932 kb on - strand, within CCNA_00362 at 378.980 kb on - strand, within CCNA_00362 at 379.000 kb on - strand, within CCNA_00362 at 379.046 kb on - strand, within CCNA_00362 at 379.048 kb on + strand, within CCNA_00362 at 379.104 kb on + strand, within CCNA_00362 at 379.112 kb on - strand, within CCNA_00362 at 379.112 kb on - strand, within CCNA_00362 at 379.120 kb on - strand, within CCNA_00362 at 379.169 kb on + strand, within CCNA_00362 at 379.176 kb on - strand, within CCNA_00362 at 379.177 kb on - strand, within CCNA_00362 at 379.255 kb on - strand at 379.285 kb on - strand at 379.296 kb on - strand at 379.296 kb on - strand at 379.330 kb on - strand at 379.342 kb on - strand at 379.342 kb on - strand at 379.342 kb on - strand at 379.342 kb on - strand at 379.342 kb on - strand at 379.342 kb on - strand at 379.366 kb on - strand at 379.366 kb on - strand at 379.409 kb on + strand, within CCNA_00363 at 379.436 kb on + strand, within CCNA_00363 at 379.473 kb on + strand, within CCNA_00363 at 379.489 kb on - strand, within CCNA_00363 at 379.513 kb on + strand, within CCNA_00363 at 379.513 kb on + strand, within CCNA_00363 at 379.514 kb on - strand, within CCNA_00363 at 379.531 kb on - strand, within CCNA_00363 at 379.531 kb on - strand, within CCNA_00363 at 379.544 kb on + strand, within CCNA_00363 at 379.544 kb on + strand, within CCNA_00363 at 379.549 kb on + strand, within CCNA_00363 at 379.552 kb on - strand, within CCNA_00363 at 379.561 kb on + strand, within CCNA_00363 at 379.569 kb on - strand, within CCNA_00363 at 379.569 kb on - strand, within CCNA_00363 at 379.602 kb on + strand, within CCNA_00363 at 379.616 kb on + strand, within CCNA_00363 at 379.621 kb on + strand, within CCNA_00363 at 379.647 kb on + strand, within CCNA_00363 at 379.659 kb on + strand, within CCNA_00363 at 379.664 kb on + strand, within CCNA_00363 at 379.782 kb on + strand, within CCNA_00363 at 379.790 kb on - strand, within CCNA_00363 at 379.837 kb on + strand, within CCNA_00363 at 379.854 kb on - strand, within CCNA_00363 at 379.854 kb on - strand, within CCNA_00363 at 379.886 kb on + strand, within CCNA_00363 at 379.906 kb on - strand, within CCNA_00363 at 379.950 kb on + strand, within CCNA_00363 at 379.958 kb on - strand, within CCNA_00363 at 380.003 kb on + strand, within CCNA_00363 at 380.043 kb on + strand at 380.043 kb on + strand at 380.043 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 378,325 - CCNA_00361 0.55 +0.4 378,361 - CCNA_00361 0.59 +0.8 378,401 + CCNA_00361 0.63 -0.1 378,453 + CCNA_00361 0.69 -1.3 378,468 + CCNA_00361 0.70 +0.4 378,499 - CCNA_00361 0.73 -0.4 378,511 - CCNA_00361 0.75 +0.4 378,520 + CCNA_00361 0.76 -0.1 378,562 - CCNA_00361 0.80 +0.3 378,562 - CCNA_00361 0.80 +0.6 378,562 - CCNA_00361 0.80 -0.4 378,577 + CCNA_00361 0.82 -0.7 378,633 - CCNA_00361 0.88 -0.1 378,648 + CCNA_00361 0.89 +0.6 378,658 + +0.6 378,698 - +0.0 378,764 - +0.5 378,833 - -0.7 378,855 + CCNA_00362 0.11 -0.3 378,855 + CCNA_00362 0.11 -1.3 378,862 + CCNA_00362 0.12 -1.4 378,893 + CCNA_00362 0.19 -0.8 378,932 - CCNA_00362 0.27 -0.1 378,980 - CCNA_00362 0.37 +1.0 379,000 - CCNA_00362 0.41 +0.1 379,046 - CCNA_00362 0.50 -0.2 379,048 + CCNA_00362 0.51 -0.3 379,104 + CCNA_00362 0.62 -1.2 379,112 - CCNA_00362 0.64 +1.4 379,112 - CCNA_00362 0.64 +0.1 379,120 - CCNA_00362 0.65 -0.1 379,169 + CCNA_00362 0.76 -0.5 379,176 - CCNA_00362 0.77 -0.5 379,177 - CCNA_00362 0.77 -0.1 379,255 - +0.3 379,285 - +0.3 379,296 - -0.6 379,296 - -0.1 379,330 - +0.9 379,342 - +0.1 379,342 - -0.5 379,342 - +0.3 379,342 - +1.8 379,342 - -1.4 379,342 - -0.3 379,366 - +0.1 379,366 - -0.1 379,409 + CCNA_00363 0.11 -0.2 379,436 + CCNA_00363 0.14 +0.3 379,473 + CCNA_00363 0.19 -1.2 379,489 - CCNA_00363 0.21 +0.4 379,513 + CCNA_00363 0.24 -0.9 379,513 + CCNA_00363 0.24 -0.1 379,514 - CCNA_00363 0.24 +0.9 379,531 - CCNA_00363 0.27 -0.8 379,531 - CCNA_00363 0.27 -0.0 379,544 + CCNA_00363 0.28 -0.1 379,544 + CCNA_00363 0.28 -0.1 379,549 + CCNA_00363 0.29 -1.5 379,552 - CCNA_00363 0.29 +1.2 379,561 + CCNA_00363 0.31 -0.2 379,569 - CCNA_00363 0.32 +0.1 379,569 - CCNA_00363 0.32 +0.3 379,602 + CCNA_00363 0.36 -1.0 379,616 + CCNA_00363 0.38 -0.3 379,621 + CCNA_00363 0.38 -0.5 379,647 + CCNA_00363 0.42 -0.2 379,659 + CCNA_00363 0.43 -0.8 379,664 + CCNA_00363 0.44 -0.7 379,782 + CCNA_00363 0.59 -0.7 379,790 - CCNA_00363 0.60 +0.6 379,837 + CCNA_00363 0.66 +0.3 379,854 - CCNA_00363 0.68 +1.3 379,854 - CCNA_00363 0.68 -1.4 379,886 + CCNA_00363 0.72 +0.2 379,906 - CCNA_00363 0.75 +1.0 379,950 + CCNA_00363 0.81 -1.9 379,958 - CCNA_00363 0.82 +0.6 380,003 + CCNA_00363 0.87 +0.4 380,043 + +0.6 380,043 + +0.4 380,043 + +0.2
Or see this region's nucleotide sequence