Strain Fitness in Escherichia coli BW25113 around b0083

Experiment: D-Galacturonic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmraZ and mraW are separated by 1 nucleotidesmraW and ftsL overlap by 4 nucleotidesftsL and ftsI are separated by 15 nucleotides b0081: mraZ - hypothetical protein (NCBI), at 89,634 to 90,092 mraZ b0082: mraW - S-adenosyl-methyltransferase (NCBI), at 90,094 to 91,035 mraW b0083: ftsL - membrane bound cell division protein at septum containing leucine zipper motif (NCBI), at 91,032 to 91,397 ftsL b0084: ftsI - transpeptidase involved in septal peptidoglycan synthesis (penicillin-binding protein 3) (NCBI), at 91,413 to 93,179 ftsI Position (kb) 91 92Strain fitness (log2 ratio) -3 -2 -1 0 1at 90.039 kb on + strand, within mraZat 90.039 kb on + strand, within mraZat 90.042 kb on + strand, within mraZat 90.042 kb on + strand, within mraZat 90.230 kb on + strand, within mraWat 90.473 kb on + strand, within mraWat 90.518 kb on + strand, within mraWat 90.544 kb on + strand, within mraWat 90.681 kb on + strand, within mraWat 90.701 kb on + strand, within mraWat 90.783 kb on + strand, within mraWat 90.887 kb on + strand, within mraWat 90.896 kb on + strand, within mraWat 90.899 kb on + strand, within mraWat 90.979 kb on + strandat 91.008 kb on + strandat 91.020 kb on + strandat 91.020 kb on + strandat 91.020 kb on + strandat 91.028 kb on + strandat 91.028 kb on + strandat 91.357 kb on + strandat 91.362 kb on + strandat 91.378 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Galacturonic Acid (C)
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90,039 + mraZ b0081 0.88 +0.4
90,039 + mraZ b0081 0.88 -0.7
90,042 + mraZ b0081 0.89 +0.0
90,042 + mraZ b0081 0.89 +0.3
90,230 + mraW b0082 0.14 +0.6
90,473 + mraW b0082 0.40 -0.1
90,518 + mraW b0082 0.45 +0.8
90,544 + mraW b0082 0.48 -0.3
90,681 + mraW b0082 0.62 -0.2
90,701 + mraW b0082 0.64 -0.3
90,783 + mraW b0082 0.73 -1.3
90,887 + mraW b0082 0.84 -1.3
90,896 + mraW b0082 0.85 +1.3
90,899 + mraW b0082 0.85 +0.6
90,979 + +0.6
91,008 + -0.8
91,020 + -3.0
91,020 + -1.0
91,020 + +0.4
91,028 + +0.6
91,028 + +0.7
91,357 + +1.6
91,362 + +0.8
91,378 + -0.5

Or see this region's nucleotide sequence