Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4542 and EX28DRAFT_4543 are separated by 11 nucleotides EX28DRAFT_4543 and EX28DRAFT_4544 are separated by 49 nucleotides EX28DRAFT_4544 and EX28DRAFT_4545 are separated by 15 nucleotides
EX28DRAFT_4542: EX28DRAFT_4542 - Bacterial nucleoid DNA-binding protein, at 24,833 to 25,105
_4542
EX28DRAFT_4543: EX28DRAFT_4543 - Protein of unknown function (DUF1481), at 25,117 to 25,809
_4543
EX28DRAFT_4544: EX28DRAFT_4544 - phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 25,859 to 27,151
_4544
EX28DRAFT_4545: EX28DRAFT_4545 - AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful), at 27,167 to 28,756
_4545
Position (kb)
25
26
27
28 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 24.859 kb on - strand at 24.895 kb on + strand, within EX28DRAFT_4542 at 24.913 kb on + strand, within EX28DRAFT_4542 at 25.006 kb on + strand, within EX28DRAFT_4542 at 25.079 kb on - strand at 25.099 kb on + strand at 25.103 kb on + strand at 25.104 kb on - strand at 25.129 kb on - strand at 25.344 kb on + strand, within EX28DRAFT_4543 at 25.344 kb on + strand, within EX28DRAFT_4543 at 25.344 kb on + strand, within EX28DRAFT_4543 at 25.345 kb on - strand, within EX28DRAFT_4543 at 25.345 kb on - strand, within EX28DRAFT_4543 at 25.345 kb on - strand, within EX28DRAFT_4543 at 25.406 kb on - strand, within EX28DRAFT_4543 at 25.406 kb on - strand, within EX28DRAFT_4543 at 25.465 kb on - strand, within EX28DRAFT_4543 at 25.679 kb on + strand, within EX28DRAFT_4543 at 25.679 kb on + strand, within EX28DRAFT_4543 at 25.679 kb on + strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.779 kb on + strand at 25.812 kb on + strand at 25.813 kb on - strand at 26.118 kb on + strand, within EX28DRAFT_4544 at 27.169 kb on - strand at 28.041 kb on + strand, within EX28DRAFT_4545 at 28.041 kb on + strand, within EX28DRAFT_4545
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 24,859 - -4.4 24,895 + EX28DRAFT_4542 0.23 -1.8 24,913 + EX28DRAFT_4542 0.29 -1.2 25,006 + EX28DRAFT_4542 0.63 -1.6 25,079 - -0.6 25,099 + -1.2 25,103 + -0.9 25,104 - -1.4 25,129 - -1.2 25,344 + EX28DRAFT_4543 0.33 +1.4 25,344 + EX28DRAFT_4543 0.33 -0.4 25,344 + EX28DRAFT_4543 0.33 +0.7 25,345 - EX28DRAFT_4543 0.33 +0.7 25,345 - EX28DRAFT_4543 0.33 +1.0 25,345 - EX28DRAFT_4543 0.33 -0.4 25,406 - EX28DRAFT_4543 0.42 -0.3 25,406 - EX28DRAFT_4543 0.42 -1.6 25,465 - EX28DRAFT_4543 0.50 -0.0 25,679 + EX28DRAFT_4543 0.81 +0.5 25,679 + EX28DRAFT_4543 0.81 +0.9 25,679 + EX28DRAFT_4543 0.81 -0.3 25,680 - EX28DRAFT_4543 0.81 -0.1 25,680 - EX28DRAFT_4543 0.81 +0.3 25,680 - EX28DRAFT_4543 0.81 -0.2 25,680 - EX28DRAFT_4543 0.81 +0.8 25,680 - EX28DRAFT_4543 0.81 +0.5 25,680 - EX28DRAFT_4543 0.81 -0.2 25,779 + +1.3 25,812 + +0.2 25,813 - -0.0 26,118 + EX28DRAFT_4544 0.20 -1.7 27,169 - -0.8 28,041 + EX28DRAFT_4545 0.55 +2.5 28,041 + EX28DRAFT_4545 0.55 -0.9
Or see this region's nucleotide sequence