Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4420 and EX28DRAFT_4421 are separated by 9 nucleotides EX28DRAFT_4421 and EX28DRAFT_4422 are separated by 74 nucleotides
EX28DRAFT_4420: EX28DRAFT_4420 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 87,709 to 88,713
_4420
EX28DRAFT_4421: EX28DRAFT_4421 - N-acetylglutamate kinase (EC 2.7.2.8), at 88,723 to 89,496
_4421
EX28DRAFT_4422: EX28DRAFT_4422 - argininosuccinate lyase, at 89,571 to 90,944
_4422
Position (kb) 88
89
90 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 87.761 kb on + strand at 87.762 kb on - strand at 87.766 kb on + strand at 87.786 kb on + strand at 87.787 kb on - strand at 87.787 kb on - strand at 88.132 kb on - strand, within EX28DRAFT_4420 at 88.327 kb on + strand, within EX28DRAFT_4420 at 88.635 kb on - strand at 88.674 kb on + strand at 88.675 kb on - strand at 88.707 kb on + strand at 88.737 kb on + strand at 88.754 kb on + strand at 89.048 kb on + strand, within EX28DRAFT_4421 at 89.484 kb on + strand at 89.484 kb on + strand at 89.490 kb on - strand at 89.515 kb on + strand at 89.549 kb on + strand at 89.670 kb on - strand at 89.738 kb on - strand, within EX28DRAFT_4422 at 89.738 kb on - strand, within EX28DRAFT_4422 at 89.933 kb on + strand, within EX28DRAFT_4422 at 90.184 kb on - strand, within EX28DRAFT_4422
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 87,761 + -1.8 87,762 - -2.5 87,766 + -2.9 87,786 + -0.4 87,787 - -2.3 87,787 - +2.4 88,132 - EX28DRAFT_4420 0.42 +2.4 88,327 + EX28DRAFT_4420 0.61 -1.8 88,635 - +0.4 88,674 + -2.4 88,675 - +0.8 88,707 + -3.0 88,737 + +2.4 88,754 + +1.4 89,048 + EX28DRAFT_4421 0.42 -1.7 89,484 + -2.3 89,484 + +0.1 89,490 - -1.8 89,515 + -1.6 89,549 + +0.2 89,670 - -1.3 89,738 - EX28DRAFT_4422 0.12 -3.6 89,738 - EX28DRAFT_4422 0.12 -1.8 89,933 + EX28DRAFT_4422 0.26 -3.1 90,184 - EX28DRAFT_4422 0.45 -3.2
Or see this region's nucleotide sequence