Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4367 and EX28DRAFT_4368 overlap by 7 nucleotides EX28DRAFT_4368 and EX28DRAFT_4369 overlap by 4 nucleotides EX28DRAFT_4369 and EX28DRAFT_4370 are separated by 44 nucleotides EX28DRAFT_4370 and EX28DRAFT_4371 are separated by 8 nucleotides
EX28DRAFT_4367: EX28DRAFT_4367 - haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED, at 34,009 to 34,608
_4367
EX28DRAFT_4368: EX28DRAFT_4368 - Predicted membrane protein, at 34,602 to 35,483
_4368
EX28DRAFT_4369: EX28DRAFT_4369 - D-tyrosyl-tRNA(Tyr) deacylase, at 35,480 to 35,917
_4369
EX28DRAFT_4370: EX28DRAFT_4370 - thioesterase domain, putative, at 35,962 to 36,903
_4370
EX28DRAFT_4371: EX28DRAFT_4371 - Esterase/lipase, at 36,912 to 37,832
_4371
Position (kb)
35
36 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 34.518 kb on + strand, within EX28DRAFT_4367 at 34.518 kb on + strand, within EX28DRAFT_4367 at 34.520 kb on + strand, within EX28DRAFT_4367 at 34.520 kb on + strand, within EX28DRAFT_4367 at 34.520 kb on + strand, within EX28DRAFT_4367 at 34.520 kb on + strand, within EX28DRAFT_4367 at 34.521 kb on - strand, within EX28DRAFT_4367 at 34.521 kb on - strand, within EX28DRAFT_4367 at 34.521 kb on - strand, within EX28DRAFT_4367 at 34.521 kb on - strand, within EX28DRAFT_4367 at 34.521 kb on - strand, within EX28DRAFT_4367 at 34.543 kb on - strand, within EX28DRAFT_4367 at 34.585 kb on + strand at 34.586 kb on - strand at 34.601 kb on + strand at 34.601 kb on + strand at 34.601 kb on + strand at 34.606 kb on + strand at 34.680 kb on - strand at 34.869 kb on + strand, within EX28DRAFT_4368 at 34.869 kb on + strand, within EX28DRAFT_4368 at 34.870 kb on - strand, within EX28DRAFT_4368 at 34.870 kb on - strand, within EX28DRAFT_4368 at 34.928 kb on + strand, within EX28DRAFT_4368 at 34.954 kb on - strand, within EX28DRAFT_4368 at 34.954 kb on - strand, within EX28DRAFT_4368 at 34.954 kb on - strand, within EX28DRAFT_4368 at 34.954 kb on - strand, within EX28DRAFT_4368 at 34.991 kb on + strand, within EX28DRAFT_4368 at 35.013 kb on + strand, within EX28DRAFT_4368 at 35.088 kb on + strand, within EX28DRAFT_4368 at 35.089 kb on - strand, within EX28DRAFT_4368 at 35.089 kb on - strand, within EX28DRAFT_4368 at 35.190 kb on + strand, within EX28DRAFT_4368 at 35.292 kb on + strand, within EX28DRAFT_4368 at 35.292 kb on + strand, within EX28DRAFT_4368 at 35.293 kb on - strand, within EX28DRAFT_4368 at 35.368 kb on - strand, within EX28DRAFT_4368 at 35.404 kb on + strand at 35.405 kb on - strand at 35.435 kb on + strand at 35.493 kb on + strand at 35.571 kb on + strand, within EX28DRAFT_4369 at 35.571 kb on + strand, within EX28DRAFT_4369 at 35.571 kb on + strand, within EX28DRAFT_4369 at 35.572 kb on - strand, within EX28DRAFT_4369 at 35.638 kb on + strand, within EX28DRAFT_4369 at 35.717 kb on - strand, within EX28DRAFT_4369 at 35.802 kb on - strand, within EX28DRAFT_4369 at 35.863 kb on + strand, within EX28DRAFT_4369 at 35.863 kb on + strand, within EX28DRAFT_4369 at 35.863 kb on + strand, within EX28DRAFT_4369 at 35.956 kb on - strand at 35.956 kb on - strand at 35.956 kb on - strand at 35.961 kb on + strand at 35.961 kb on + strand at 35.962 kb on - strand at 35.962 kb on - strand at 35.962 kb on - strand at 35.965 kb on + strand at 35.965 kb on + strand at 35.965 kb on + strand at 35.966 kb on - strand at 35.966 kb on - strand at 36.010 kb on + strand at 36.010 kb on + strand at 36.011 kb on - strand at 36.145 kb on + strand, within EX28DRAFT_4370 at 36.145 kb on + strand, within EX28DRAFT_4370 at 36.146 kb on - strand, within EX28DRAFT_4370 at 36.146 kb on - strand, within EX28DRAFT_4370 at 36.146 kb on - strand, within EX28DRAFT_4370 at 36.225 kb on - strand, within EX28DRAFT_4370 at 36.411 kb on + strand, within EX28DRAFT_4370 at 36.411 kb on + strand, within EX28DRAFT_4370 at 36.412 kb on - strand, within EX28DRAFT_4370 at 36.505 kb on + strand, within EX28DRAFT_4370 at 36.506 kb on - strand, within EX28DRAFT_4370 at 36.506 kb on - strand, within EX28DRAFT_4370 at 36.522 kb on + strand, within EX28DRAFT_4370 at 36.687 kb on + strand, within EX28DRAFT_4370 at 36.783 kb on - strand, within EX28DRAFT_4370 at 36.842 kb on - strand at 36.842 kb on - strand at 36.842 kb on - strand at 36.842 kb on - strand at 36.842 kb on - strand at 36.842 kb on - strand at 36.842 kb on - strand at 36.901 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 34,518 + EX28DRAFT_4367 0.85 -0.1 34,518 + EX28DRAFT_4367 0.85 -0.2 34,520 + EX28DRAFT_4367 0.85 -1.1 34,520 + EX28DRAFT_4367 0.85 -0.5 34,520 + EX28DRAFT_4367 0.85 -0.8 34,520 + EX28DRAFT_4367 0.85 +0.1 34,521 - EX28DRAFT_4367 0.85 +0.1 34,521 - EX28DRAFT_4367 0.85 +0.3 34,521 - EX28DRAFT_4367 0.85 -0.2 34,521 - EX28DRAFT_4367 0.85 +1.0 34,521 - EX28DRAFT_4367 0.85 -0.0 34,543 - EX28DRAFT_4367 0.89 +1.1 34,585 + -0.4 34,586 - +0.6 34,601 + -0.4 34,601 + -1.7 34,601 + +0.2 34,606 + +0.1 34,680 - +0.2 34,869 + EX28DRAFT_4368 0.30 -0.3 34,869 + EX28DRAFT_4368 0.30 -0.3 34,870 - EX28DRAFT_4368 0.30 -0.5 34,870 - EX28DRAFT_4368 0.30 +0.3 34,928 + EX28DRAFT_4368 0.37 -3.1 34,954 - EX28DRAFT_4368 0.40 -1.7 34,954 - EX28DRAFT_4368 0.40 -0.0 34,954 - EX28DRAFT_4368 0.40 +3.1 34,954 - EX28DRAFT_4368 0.40 +0.1 34,991 + EX28DRAFT_4368 0.44 +0.2 35,013 + EX28DRAFT_4368 0.47 +0.3 35,088 + EX28DRAFT_4368 0.55 +1.1 35,089 - EX28DRAFT_4368 0.55 +0.1 35,089 - EX28DRAFT_4368 0.55 +0.4 35,190 + EX28DRAFT_4368 0.67 -0.2 35,292 + EX28DRAFT_4368 0.78 +0.3 35,292 + EX28DRAFT_4368 0.78 +0.9 35,293 - EX28DRAFT_4368 0.78 +1.3 35,368 - EX28DRAFT_4368 0.87 -0.6 35,404 + +0.4 35,405 - -2.9 35,435 + -0.4 35,493 + -0.6 35,571 + EX28DRAFT_4369 0.21 -0.3 35,571 + EX28DRAFT_4369 0.21 -0.3 35,571 + EX28DRAFT_4369 0.21 +1.0 35,572 - EX28DRAFT_4369 0.21 +0.2 35,638 + EX28DRAFT_4369 0.36 -0.5 35,717 - EX28DRAFT_4369 0.54 -1.7 35,802 - EX28DRAFT_4369 0.74 -1.4 35,863 + EX28DRAFT_4369 0.87 -0.8 35,863 + EX28DRAFT_4369 0.87 -0.8 35,863 + EX28DRAFT_4369 0.87 +0.3 35,956 - +0.3 35,956 - -1.1 35,956 - -0.1 35,961 + +0.5 35,961 + +0.3 35,962 - +0.8 35,962 - -0.3 35,962 - +0.6 35,965 + -1.2 35,965 + -0.1 35,965 + +0.2 35,966 - -0.3 35,966 - -0.8 36,010 + -1.0 36,010 + -0.1 36,011 - +0.0 36,145 + EX28DRAFT_4370 0.19 -0.1 36,145 + EX28DRAFT_4370 0.19 +0.3 36,146 - EX28DRAFT_4370 0.20 -2.6 36,146 - EX28DRAFT_4370 0.20 -0.5 36,146 - EX28DRAFT_4370 0.20 +0.0 36,225 - EX28DRAFT_4370 0.28 -0.1 36,411 + EX28DRAFT_4370 0.48 -0.1 36,411 + EX28DRAFT_4370 0.48 -0.8 36,412 - EX28DRAFT_4370 0.48 +0.1 36,505 + EX28DRAFT_4370 0.58 +0.9 36,506 - EX28DRAFT_4370 0.58 -0.2 36,506 - EX28DRAFT_4370 0.58 -0.2 36,522 + EX28DRAFT_4370 0.59 +0.9 36,687 + EX28DRAFT_4370 0.77 -0.4 36,783 - EX28DRAFT_4370 0.87 -0.0 36,842 - -0.7 36,842 - -0.1 36,842 - +0.1 36,842 - +0.1 36,842 - +0.1 36,842 - +1.4 36,842 - +0.2 36,901 + -0.2
Or see this region's nucleotide sequence