Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3897 and EX28DRAFT_3898 are separated by 54 nucleotides EX28DRAFT_3898 and EX28DRAFT_3899 are separated by 19 nucleotides EX28DRAFT_3899 and EX28DRAFT_3900 are separated by 37 nucleotides EX28DRAFT_3900 and EX28DRAFT_3901 are separated by 3 nucleotides
EX28DRAFT_3897: EX28DRAFT_3897 - guanylate kinase (EC 2.7.4.8), at 105,144 to 105,767
_3897
EX28DRAFT_3898: EX28DRAFT_3898 - DNA-directed RNA polymerase subunit omega (EC 2.7.7.6), at 105,822 to 106,097
_3898
EX28DRAFT_3899: EX28DRAFT_3899 - (p)ppGpp synthetase, RelA/SpoT family, at 106,117 to 108,231
_3899
EX28DRAFT_3900: EX28DRAFT_3900 - SpoU rRNA Methylase family/SpoU, rRNA methylase, C-terminal, at 108,269 to 108,925
_3900
EX28DRAFT_3901: EX28DRAFT_3901 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 108,929 to 111,010
_3901
Position (kb)
106
107
108
109 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 105.817 kb on + strand at 105.821 kb on + strand at 105.965 kb on + strand, within EX28DRAFT_3898 at 107.922 kb on + strand, within EX28DRAFT_3899 at 107.923 kb on - strand, within EX28DRAFT_3899 at 107.977 kb on - strand, within EX28DRAFT_3899 at 108.010 kb on - strand, within EX28DRAFT_3899 at 108.018 kb on + strand, within EX28DRAFT_3899 at 108.018 kb on + strand, within EX28DRAFT_3899 at 108.018 kb on + strand, within EX28DRAFT_3899 at 108.171 kb on + strand at 108.230 kb on - strand at 108.230 kb on - strand at 108.251 kb on + strand at 108.259 kb on + strand at 108.319 kb on + strand at 108.319 kb on + strand at 108.319 kb on + strand at 108.320 kb on - strand at 108.320 kb on - strand at 108.711 kb on - strand, within EX28DRAFT_3900 at 108.746 kb on + strand, within EX28DRAFT_3900 at 108.747 kb on - strand, within EX28DRAFT_3900 at 109.109 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 105,817 + -1.7 105,821 + -0.8 105,965 + EX28DRAFT_3898 0.52 -1.6 107,922 + EX28DRAFT_3899 0.85 -0.5 107,923 - EX28DRAFT_3899 0.85 -1.6 107,977 - EX28DRAFT_3899 0.88 -1.2 108,010 - EX28DRAFT_3899 0.90 -0.1 108,018 + EX28DRAFT_3899 0.90 -2.1 108,018 + EX28DRAFT_3899 0.90 +0.1 108,018 + EX28DRAFT_3899 0.90 -0.8 108,171 + +0.3 108,230 - -2.7 108,230 - -0.0 108,251 + +0.1 108,259 + -0.3 108,319 + -0.8 108,319 + +0.3 108,319 + -1.5 108,320 - -4.0 108,320 - +0.7 108,711 - EX28DRAFT_3900 0.67 -1.5 108,746 + EX28DRAFT_3900 0.73 +0.1 108,747 - EX28DRAFT_3900 0.73 -0.3 109,109 + -2.2
Or see this region's nucleotide sequence