Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3733 and EX28DRAFT_3734 are separated by 59 nucleotides EX28DRAFT_3734 and EX28DRAFT_3735 are separated by 176 nucleotides EX28DRAFT_3735 and EX28DRAFT_3736 overlap by 11 nucleotides EX28DRAFT_3736 and EX28DRAFT_3737 are separated by 73 nucleotides
EX28DRAFT_3733: EX28DRAFT_3733 - glutamyl-queuosine tRNA(Asp) synthetase, at 408,021 to 408,911
_3733
EX28DRAFT_3734: EX28DRAFT_3734 - transcriptional regulator, TraR/DksA family, at 408,971 to 409,426
_3734
EX28DRAFT_3735: EX28DRAFT_3735 - sugar fermentation stimulation protein, at 409,603 to 410,307
_3735
EX28DRAFT_3736: EX28DRAFT_3736 - 2'-5' RNA ligase, at 410,297 to 410,851
_3736
EX28DRAFT_3737: EX28DRAFT_3737 - ATP-dependent helicase HrpB, at 410,925 to 413,354
_3737
Position (kb)
409
410
411 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 408.616 kb on + strand, within EX28DRAFT_3733 at 408.616 kb on + strand, within EX28DRAFT_3733 at 408.616 kb on + strand, within EX28DRAFT_3733 at 408.617 kb on - strand, within EX28DRAFT_3733 at 408.823 kb on + strand at 408.843 kb on + strand at 408.859 kb on - strand at 408.859 kb on - strand at 408.894 kb on - strand at 408.895 kb on + strand at 408.896 kb on - strand at 408.896 kb on - strand at 408.911 kb on + strand at 408.911 kb on + strand at 408.911 kb on + strand at 408.957 kb on - strand at 408.969 kb on + strand at 408.970 kb on - strand at 409.487 kb on - strand at 409.487 kb on - strand at 409.487 kb on - strand at 409.487 kb on - strand at 409.491 kb on - strand at 409.491 kb on - strand at 409.517 kb on - strand at 409.521 kb on - strand at 409.521 kb on - strand at 409.526 kb on + strand at 409.527 kb on - strand at 409.604 kb on + strand at 409.606 kb on + strand at 409.607 kb on - strand at 409.607 kb on - strand at 409.607 kb on - strand at 409.639 kb on - strand at 409.639 kb on - strand at 409.639 kb on - strand at 409.662 kb on + strand at 409.720 kb on - strand, within EX28DRAFT_3735 at 409.816 kb on + strand, within EX28DRAFT_3735 at 409.817 kb on - strand, within EX28DRAFT_3735 at 409.910 kb on + strand, within EX28DRAFT_3735 at 409.911 kb on - strand, within EX28DRAFT_3735 at 409.970 kb on + strand, within EX28DRAFT_3735 at 409.970 kb on + strand, within EX28DRAFT_3735 at 409.986 kb on + strand, within EX28DRAFT_3735 at 409.986 kb on + strand, within EX28DRAFT_3735 at 410.077 kb on - strand, within EX28DRAFT_3735 at 410.077 kb on - strand, within EX28DRAFT_3735 at 410.112 kb on + strand, within EX28DRAFT_3735 at 410.113 kb on - strand, within EX28DRAFT_3735 at 410.120 kb on + strand, within EX28DRAFT_3735 at 410.120 kb on + strand, within EX28DRAFT_3735 at 410.120 kb on + strand, within EX28DRAFT_3735 at 410.133 kb on + strand, within EX28DRAFT_3735 at 410.476 kb on + strand, within EX28DRAFT_3736 at 410.477 kb on - strand, within EX28DRAFT_3736 at 410.872 kb on - strand at 410.872 kb on - strand at 410.884 kb on + strand at 410.892 kb on - strand at 410.907 kb on - strand at 410.974 kb on + strand at 410.975 kb on - strand at 410.975 kb on - strand at 411.203 kb on + strand, within EX28DRAFT_3737 at 411.231 kb on - strand, within EX28DRAFT_3737
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 408,616 + EX28DRAFT_3733 0.67 -3.5 408,616 + EX28DRAFT_3733 0.67 +0.4 408,616 + EX28DRAFT_3733 0.67 +0.4 408,617 - EX28DRAFT_3733 0.67 -0.3 408,823 + +0.8 408,843 + +1.8 408,859 - +0.5 408,859 - +0.7 408,894 - -0.5 408,895 + -1.6 408,896 - -0.7 408,896 - -1.0 408,911 + -0.5 408,911 + -1.9 408,911 + -0.0 408,957 - -1.9 408,969 + -3.2 408,970 - -1.9 409,487 - -3.2 409,487 - -1.8 409,487 - -1.7 409,487 - -1.7 409,491 - -2.1 409,491 - -3.6 409,517 - -1.2 409,521 - -0.3 409,521 - -0.1 409,526 + +0.3 409,527 - +0.3 409,604 + -0.5 409,606 + +0.6 409,607 - -0.1 409,607 - +0.4 409,607 - +1.0 409,639 - +1.3 409,639 - +0.5 409,639 - +0.1 409,662 + -0.5 409,720 - EX28DRAFT_3735 0.17 +1.1 409,816 + EX28DRAFT_3735 0.30 +1.2 409,817 - EX28DRAFT_3735 0.30 +0.0 409,910 + EX28DRAFT_3735 0.44 +0.2 409,911 - EX28DRAFT_3735 0.44 -2.7 409,970 + EX28DRAFT_3735 0.52 -0.1 409,970 + EX28DRAFT_3735 0.52 +1.3 409,986 + EX28DRAFT_3735 0.54 -0.5 409,986 + EX28DRAFT_3735 0.54 -0.4 410,077 - EX28DRAFT_3735 0.67 +0.6 410,077 - EX28DRAFT_3735 0.67 +0.2 410,112 + EX28DRAFT_3735 0.72 +0.3 410,113 - EX28DRAFT_3735 0.72 -0.7 410,120 + EX28DRAFT_3735 0.73 +0.3 410,120 + EX28DRAFT_3735 0.73 +1.6 410,120 + EX28DRAFT_3735 0.73 -0.4 410,133 + EX28DRAFT_3735 0.75 -2.0 410,476 + EX28DRAFT_3736 0.32 +0.6 410,477 - EX28DRAFT_3736 0.32 -0.2 410,872 - -0.4 410,872 - -0.2 410,884 + +1.1 410,892 - +0.8 410,907 - +0.7 410,974 + -0.1 410,975 - +0.3 410,975 - -0.3 411,203 + EX28DRAFT_3737 0.11 -0.1 411,231 - EX28DRAFT_3737 0.13 -1.0
Or see this region's nucleotide sequence