Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3670 and EX28DRAFT_3671 are separated by 43 nucleotides EX28DRAFT_3671 and EX28DRAFT_3672 are separated by 574 nucleotides EX28DRAFT_3672 and EX28DRAFT_3673 are separated by 380 nucleotides
EX28DRAFT_3670: EX28DRAFT_3670 - 2-isopropylmalate synthase (EC 2.3.3.13), at 336,886 to 338,457
_3670
EX28DRAFT_3671: EX28DRAFT_3671 - RNA, at 338,501 to 338,649
_3671
EX28DRAFT_3672: EX28DRAFT_3672 - Transcriptional regulator, at 339,224 to 340,186
_3672
EX28DRAFT_3673: EX28DRAFT_3673 - acetolactate synthase, large subunit (EC 2.2.1.6), at 340,567 to 342,291
_3673
Position (kb)
339
340
341 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 338.346 kb on + strand at 338.427 kb on + strand at 338.427 kb on + strand at 338.427 kb on + strand at 338.428 kb on - strand at 338.463 kb on - strand at 338.500 kb on + strand at 338.501 kb on - strand at 338.537 kb on - strand, within EX28DRAFT_3671 at 338.600 kb on + strand, within EX28DRAFT_3671 at 338.603 kb on + strand, within EX28DRAFT_3671 at 338.604 kb on - strand, within EX28DRAFT_3671 at 338.604 kb on - strand, within EX28DRAFT_3671 at 338.604 kb on - strand, within EX28DRAFT_3671 at 338.604 kb on - strand, within EX28DRAFT_3671 at 338.659 kb on + strand at 338.668 kb on - strand at 338.755 kb on - strand at 338.832 kb on - strand at 338.904 kb on + strand at 338.926 kb on + strand at 339.037 kb on + strand at 339.083 kb on + strand at 339.194 kb on + strand at 339.259 kb on + strand at 339.298 kb on - strand at 339.355 kb on + strand, within EX28DRAFT_3672 at 339.475 kb on + strand, within EX28DRAFT_3672 at 339.615 kb on - strand, within EX28DRAFT_3672 at 339.615 kb on - strand, within EX28DRAFT_3672 at 339.632 kb on + strand, within EX28DRAFT_3672 at 339.677 kb on - strand, within EX28DRAFT_3672 at 339.860 kb on + strand, within EX28DRAFT_3672 at 339.860 kb on + strand, within EX28DRAFT_3672 at 339.860 kb on + strand, within EX28DRAFT_3672 at 339.862 kb on + strand, within EX28DRAFT_3672 at 339.863 kb on - strand, within EX28DRAFT_3672 at 339.896 kb on + strand, within EX28DRAFT_3672 at 339.897 kb on - strand, within EX28DRAFT_3672 at 339.897 kb on - strand, within EX28DRAFT_3672 at 339.897 kb on - strand, within EX28DRAFT_3672 at 339.897 kb on - strand, within EX28DRAFT_3672 at 339.934 kb on + strand, within EX28DRAFT_3672 at 339.953 kb on + strand, within EX28DRAFT_3672 at 339.979 kb on - strand, within EX28DRAFT_3672 at 339.982 kb on - strand, within EX28DRAFT_3672 at 340.086 kb on + strand, within EX28DRAFT_3672 at 340.242 kb on - strand at 340.339 kb on - strand at 340.369 kb on - strand at 340.405 kb on + strand at 340.416 kb on + strand at 340.483 kb on + strand at 340.484 kb on - strand at 340.486 kb on - strand at 340.541 kb on + strand at 340.541 kb on + strand at 340.542 kb on - strand at 340.622 kb on + strand at 340.666 kb on + strand at 340.667 kb on - strand at 340.667 kb on - strand at 340.667 kb on - strand at 340.709 kb on + strand at 340.731 kb on - strand at 340.778 kb on + strand, within EX28DRAFT_3673 at 340.779 kb on - strand, within EX28DRAFT_3673 at 340.781 kb on + strand, within EX28DRAFT_3673 at 340.834 kb on + strand, within EX28DRAFT_3673 at 340.835 kb on - strand, within EX28DRAFT_3673 at 340.835 kb on - strand, within EX28DRAFT_3673 at 341.053 kb on - strand, within EX28DRAFT_3673 at 341.086 kb on + strand, within EX28DRAFT_3673 at 341.087 kb on - strand, within EX28DRAFT_3673 at 341.119 kb on + strand, within EX28DRAFT_3673 at 341.119 kb on + strand, within EX28DRAFT_3673
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 338,346 + -2.1 338,427 + -0.9 338,427 + +0.3 338,427 + -0.9 338,428 - -4.5 338,463 - -3.3 338,500 + -4.3 338,501 - -0.6 338,537 - EX28DRAFT_3671 0.24 +0.1 338,600 + EX28DRAFT_3671 0.66 -4.6 338,603 + EX28DRAFT_3671 0.68 -4.8 338,604 - EX28DRAFT_3671 0.69 -0.7 338,604 - EX28DRAFT_3671 0.69 -4.1 338,604 - EX28DRAFT_3671 0.69 -0.0 338,604 - EX28DRAFT_3671 0.69 -1.6 338,659 + -2.3 338,668 - -1.7 338,755 - +0.0 338,832 - -0.4 338,904 + +0.3 338,926 + +0.7 339,037 + -2.7 339,083 + -0.2 339,194 + -1.1 339,259 + +0.0 339,298 - +0.3 339,355 + EX28DRAFT_3672 0.14 -0.3 339,475 + EX28DRAFT_3672 0.26 -0.6 339,615 - EX28DRAFT_3672 0.41 +0.6 339,615 - EX28DRAFT_3672 0.41 +0.1 339,632 + EX28DRAFT_3672 0.42 -0.2 339,677 - EX28DRAFT_3672 0.47 -0.6 339,860 + EX28DRAFT_3672 0.66 -0.0 339,860 + EX28DRAFT_3672 0.66 -2.9 339,860 + EX28DRAFT_3672 0.66 -0.1 339,862 + EX28DRAFT_3672 0.66 -0.3 339,863 - EX28DRAFT_3672 0.66 -0.0 339,896 + EX28DRAFT_3672 0.70 +0.1 339,897 - EX28DRAFT_3672 0.70 -0.9 339,897 - EX28DRAFT_3672 0.70 +0.0 339,897 - EX28DRAFT_3672 0.70 -0.6 339,897 - EX28DRAFT_3672 0.70 +1.1 339,934 + EX28DRAFT_3672 0.74 +0.3 339,953 + EX28DRAFT_3672 0.76 +0.3 339,979 - EX28DRAFT_3672 0.78 -0.7 339,982 - EX28DRAFT_3672 0.79 +0.0 340,086 + EX28DRAFT_3672 0.90 +0.8 340,242 - -0.1 340,339 - -0.3 340,369 - -1.0 340,405 + +0.5 340,416 + -0.4 340,483 + +1.3 340,484 - +0.4 340,486 - -0.3 340,541 + -0.1 340,541 + -0.4 340,542 - -0.2 340,622 + +0.1 340,666 + -0.1 340,667 - +0.1 340,667 - -2.6 340,667 - +0.6 340,709 + -0.7 340,731 - +0.6 340,778 + EX28DRAFT_3673 0.12 -0.1 340,779 - EX28DRAFT_3673 0.12 +0.7 340,781 + EX28DRAFT_3673 0.12 -0.8 340,834 + EX28DRAFT_3673 0.15 -0.6 340,835 - EX28DRAFT_3673 0.16 +0.7 340,835 - EX28DRAFT_3673 0.16 -1.7 341,053 - EX28DRAFT_3673 0.28 -1.4 341,086 + EX28DRAFT_3673 0.30 -3.5 341,087 - EX28DRAFT_3673 0.30 -0.7 341,119 + EX28DRAFT_3673 0.32 -0.3 341,119 + EX28DRAFT_3673 0.32 -0.5
Or see this region's nucleotide sequence