Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3612 and EX28DRAFT_3613 are separated by 218 nucleotides EX28DRAFT_3613 and EX28DRAFT_3614 are separated by 24 nucleotides EX28DRAFT_3614 and EX28DRAFT_3615 are separated by 1 nucleotides EX28DRAFT_3615 and EX28DRAFT_3616 are separated by 3 nucleotides
EX28DRAFT_3612: EX28DRAFT_3612 - RNA methyltransferase, TrmH family, group 1, at 271,320 to 272,006
_3612
EX28DRAFT_3613: EX28DRAFT_3613 - RNA, at 272,225 to 272,342
_3613
EX28DRAFT_3614: EX28DRAFT_3614 - aspartate kinase (EC 2.7.2.4), at 272,367 to 274,829
_3614
EX28DRAFT_3615: EX28DRAFT_3615 - homoserine kinase (EC 2.7.1.39), at 274,831 to 275,760
_3615
EX28DRAFT_3616: EX28DRAFT_3616 - threonine synthase, at 275,764 to 277,050
_3616
Position (kb)
272
273
274
275 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 271.412 kb on - strand, within EX28DRAFT_3612 at 271.412 kb on - strand, within EX28DRAFT_3612 at 271.581 kb on + strand, within EX28DRAFT_3612 at 271.581 kb on + strand, within EX28DRAFT_3612 at 271.751 kb on - strand, within EX28DRAFT_3612 at 271.751 kb on - strand, within EX28DRAFT_3612 at 271.770 kb on + strand, within EX28DRAFT_3612 at 271.770 kb on + strand, within EX28DRAFT_3612 at 271.771 kb on - strand, within EX28DRAFT_3612 at 271.783 kb on + strand, within EX28DRAFT_3612 at 271.793 kb on - strand, within EX28DRAFT_3612 at 271.793 kb on - strand, within EX28DRAFT_3612 at 271.795 kb on + strand, within EX28DRAFT_3612 at 271.796 kb on - strand, within EX28DRAFT_3612 at 271.874 kb on - strand, within EX28DRAFT_3612 at 271.874 kb on - strand, within EX28DRAFT_3612 at 272.146 kb on + strand at 272.192 kb on + strand at 272.193 kb on - strand at 272.206 kb on + strand at 272.244 kb on + strand, within EX28DRAFT_3613 at 272.245 kb on - strand, within EX28DRAFT_3613 at 272.247 kb on + strand, within EX28DRAFT_3613 at 272.247 kb on + strand, within EX28DRAFT_3613 at 272.247 kb on + strand, within EX28DRAFT_3613 at 272.248 kb on - strand, within EX28DRAFT_3613 at 272.271 kb on + strand, within EX28DRAFT_3613 at 272.320 kb on + strand, within EX28DRAFT_3613 at 272.321 kb on - strand, within EX28DRAFT_3613 at 272.321 kb on - strand, within EX28DRAFT_3613 at 272.360 kb on + strand at 272.391 kb on - strand at 272.574 kb on - strand at 272.757 kb on - strand, within EX28DRAFT_3614 at 272.845 kb on - strand, within EX28DRAFT_3614 at 273.040 kb on + strand, within EX28DRAFT_3614 at 273.064 kb on - strand, within EX28DRAFT_3614 at 273.064 kb on - strand, within EX28DRAFT_3614 at 273.142 kb on - strand, within EX28DRAFT_3614 at 273.381 kb on - strand, within EX28DRAFT_3614 at 273.381 kb on - strand, within EX28DRAFT_3614 at 273.396 kb on - strand, within EX28DRAFT_3614 at 273.396 kb on - strand, within EX28DRAFT_3614 at 273.396 kb on - strand, within EX28DRAFT_3614 at 273.645 kb on - strand, within EX28DRAFT_3614 at 273.852 kb on - strand, within EX28DRAFT_3614 at 274.129 kb on - strand, within EX28DRAFT_3614 at 274.132 kb on - strand, within EX28DRAFT_3614 at 274.180 kb on - strand, within EX28DRAFT_3614 at 274.292 kb on + strand, within EX28DRAFT_3614 at 274.293 kb on - strand, within EX28DRAFT_3614 at 274.415 kb on - strand, within EX28DRAFT_3614 at 274.587 kb on - strand at 274.600 kb on - strand at 274.707 kb on + strand at 274.717 kb on - strand at 274.720 kb on - strand at 274.820 kb on - strand at 275.019 kb on + strand, within EX28DRAFT_3615 at 275.683 kb on - strand at 275.719 kb on - strand at 275.758 kb on + strand at 275.758 kb on + strand at 275.762 kb on + strand at 275.763 kb on - strand at 275.763 kb on - strand at 275.774 kb on - strand at 275.774 kb on - strand at 275.774 kb on - strand at 275.782 kb on - strand at 275.790 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 271,412 - EX28DRAFT_3612 0.13 -0.2 271,412 - EX28DRAFT_3612 0.13 -0.2 271,581 + EX28DRAFT_3612 0.38 -0.3 271,581 + EX28DRAFT_3612 0.38 -0.7 271,751 - EX28DRAFT_3612 0.63 +0.3 271,751 - EX28DRAFT_3612 0.63 +0.6 271,770 + EX28DRAFT_3612 0.66 +0.3 271,770 + EX28DRAFT_3612 0.66 +0.2 271,771 - EX28DRAFT_3612 0.66 -0.5 271,783 + EX28DRAFT_3612 0.67 +0.1 271,793 - EX28DRAFT_3612 0.69 +0.1 271,793 - EX28DRAFT_3612 0.69 +0.4 271,795 + EX28DRAFT_3612 0.69 +0.2 271,796 - EX28DRAFT_3612 0.69 +0.7 271,874 - EX28DRAFT_3612 0.81 +0.1 271,874 - EX28DRAFT_3612 0.81 +1.3 272,146 + -0.1 272,192 + -1.3 272,193 - -0.7 272,206 + +0.6 272,244 + EX28DRAFT_3613 0.16 -0.0 272,245 - EX28DRAFT_3613 0.17 -1.3 272,247 + EX28DRAFT_3613 0.19 -1.9 272,247 + EX28DRAFT_3613 0.19 -2.2 272,247 + EX28DRAFT_3613 0.19 -3.0 272,248 - EX28DRAFT_3613 0.19 +0.7 272,271 + EX28DRAFT_3613 0.39 +0.0 272,320 + EX28DRAFT_3613 0.81 +2.1 272,321 - EX28DRAFT_3613 0.81 -4.7 272,321 - EX28DRAFT_3613 0.81 -4.0 272,360 + +2.3 272,391 - +0.4 272,574 - -0.8 272,757 - EX28DRAFT_3614 0.16 -4.2 272,845 - EX28DRAFT_3614 0.19 +0.3 273,040 + EX28DRAFT_3614 0.27 -1.0 273,064 - EX28DRAFT_3614 0.28 -2.1 273,064 - EX28DRAFT_3614 0.28 -2.9 273,142 - EX28DRAFT_3614 0.31 -2.0 273,381 - EX28DRAFT_3614 0.41 +2.3 273,381 - EX28DRAFT_3614 0.41 -1.1 273,396 - EX28DRAFT_3614 0.42 +2.3 273,396 - EX28DRAFT_3614 0.42 -2.0 273,396 - EX28DRAFT_3614 0.42 -0.5 273,645 - EX28DRAFT_3614 0.52 -3.3 273,852 - EX28DRAFT_3614 0.60 -2.4 274,129 - EX28DRAFT_3614 0.72 -3.1 274,132 - EX28DRAFT_3614 0.72 -0.9 274,180 - EX28DRAFT_3614 0.74 -2.0 274,292 + EX28DRAFT_3614 0.78 -3.4 274,293 - EX28DRAFT_3614 0.78 -0.0 274,415 - EX28DRAFT_3614 0.83 -3.2 274,587 - -0.9 274,600 - -1.4 274,707 + +0.7 274,717 - -0.5 274,720 - -4.0 274,820 - -0.7 275,019 + EX28DRAFT_3615 0.20 -1.0 275,683 - +1.0 275,719 - -0.3 275,758 + -2.3 275,758 + -1.0 275,762 + +1.3 275,763 - +0.4 275,763 - +0.7 275,774 - -0.3 275,774 - +0.7 275,774 - -0.3 275,782 - -1.2 275,790 + -1.0
Or see this region's nucleotide sequence