Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3536 and EX28DRAFT_3537 are separated by 191 nucleotides EX28DRAFT_3537 and EX28DRAFT_3538 are separated by 179 nucleotides EX28DRAFT_3538 and EX28DRAFT_3539 are separated by 17 nucleotides
EX28DRAFT_3536: EX28DRAFT_3536 - Carbon starvation protein, predicted membrane protein, at 198,617 to 200,770
_3536
EX28DRAFT_3537: EX28DRAFT_3537 - Predicted transcriptional regulator, at 200,962 to 201,879
_3537
EX28DRAFT_3538: EX28DRAFT_3538 - 4-hydroxyphenylacetate 3-monooxygenase, reductase component, at 202,059 to 202,571
_3538
EX28DRAFT_3539: EX28DRAFT_3539 - 4-hydroxyphenylacetate 3-monooxygenase, oxygenase component, at 202,589 to 204,151
_3539
Position (kb) 200
201
202 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 199.969 kb on + strand, within EX28DRAFT_3536 at 199.970 kb on - strand, within EX28DRAFT_3536 at 199.970 kb on - strand, within EX28DRAFT_3536 at 199.996 kb on + strand, within EX28DRAFT_3536 at 200.064 kb on + strand, within EX28DRAFT_3536 at 200.064 kb on + strand, within EX28DRAFT_3536 at 200.089 kb on + strand, within EX28DRAFT_3536 at 200.139 kb on + strand, within EX28DRAFT_3536 at 200.140 kb on - strand, within EX28DRAFT_3536 at 200.140 kb on - strand, within EX28DRAFT_3536 at 200.365 kb on + strand, within EX28DRAFT_3536 at 200.509 kb on - strand, within EX28DRAFT_3536 at 200.526 kb on + strand, within EX28DRAFT_3536 at 200.526 kb on + strand, within EX28DRAFT_3536 at 200.527 kb on - strand, within EX28DRAFT_3536 at 200.601 kb on + strand at 200.601 kb on + strand at 200.602 kb on - strand at 200.610 kb on + strand at 200.613 kb on + strand at 200.632 kb on - strand at 200.669 kb on + strand at 200.669 kb on + strand at 200.670 kb on - strand at 200.761 kb on + strand at 200.765 kb on - strand at 200.773 kb on + strand at 200.788 kb on + strand at 200.793 kb on - strand at 200.827 kb on + strand at 200.828 kb on - strand at 200.834 kb on + strand at 200.995 kb on - strand at 201.011 kb on + strand at 201.027 kb on + strand at 201.084 kb on - strand, within EX28DRAFT_3537 at 201.197 kb on + strand, within EX28DRAFT_3537 at 201.210 kb on + strand, within EX28DRAFT_3537 at 201.304 kb on - strand, within EX28DRAFT_3537 at 201.333 kb on + strand, within EX28DRAFT_3537 at 201.555 kb on + strand, within EX28DRAFT_3537 at 201.714 kb on + strand, within EX28DRAFT_3537 at 201.742 kb on + strand, within EX28DRAFT_3537 at 201.820 kb on + strand at 201.879 kb on + strand at 201.880 kb on - strand at 201.893 kb on - strand at 201.894 kb on + strand at 201.898 kb on + strand at 201.898 kb on + strand at 201.903 kb on - strand at 201.975 kb on + strand at 201.975 kb on + strand at 201.976 kb on - strand at 202.002 kb on + strand at 202.111 kb on + strand, within EX28DRAFT_3538 at 202.111 kb on + strand, within EX28DRAFT_3538 at 202.112 kb on - strand, within EX28DRAFT_3538 at 202.145 kb on + strand, within EX28DRAFT_3538 at 202.166 kb on - strand, within EX28DRAFT_3538 at 202.166 kb on - strand, within EX28DRAFT_3538 at 202.564 kb on - strand at 202.771 kb on - strand, within EX28DRAFT_3539
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 199,969 + EX28DRAFT_3536 0.63 +0.4 199,970 - EX28DRAFT_3536 0.63 +1.0 199,970 - EX28DRAFT_3536 0.63 +0.2 199,996 + EX28DRAFT_3536 0.64 -0.2 200,064 + EX28DRAFT_3536 0.67 +0.2 200,064 + EX28DRAFT_3536 0.67 +0.0 200,089 + EX28DRAFT_3536 0.68 +0.1 200,139 + EX28DRAFT_3536 0.71 -0.0 200,140 - EX28DRAFT_3536 0.71 +0.5 200,140 - EX28DRAFT_3536 0.71 +0.2 200,365 + EX28DRAFT_3536 0.81 -0.4 200,509 - EX28DRAFT_3536 0.88 -0.1 200,526 + EX28DRAFT_3536 0.89 +0.5 200,526 + EX28DRAFT_3536 0.89 +0.6 200,527 - EX28DRAFT_3536 0.89 -1.1 200,601 + -1.7 200,601 + -0.3 200,602 - +0.3 200,610 + +0.0 200,613 + +1.8 200,632 - +0.1 200,669 + -0.9 200,669 + +0.5 200,670 - +0.3 200,761 + +0.1 200,765 - -1.1 200,773 + -0.8 200,788 + +0.2 200,793 - -0.9 200,827 + +0.9 200,828 - -0.7 200,834 + -0.4 200,995 - -0.5 201,011 + +0.2 201,027 + -0.3 201,084 - EX28DRAFT_3537 0.13 -0.8 201,197 + EX28DRAFT_3537 0.26 -2.8 201,210 + EX28DRAFT_3537 0.27 -1.3 201,304 - EX28DRAFT_3537 0.37 +0.4 201,333 + EX28DRAFT_3537 0.40 -0.9 201,555 + EX28DRAFT_3537 0.65 +1.2 201,714 + EX28DRAFT_3537 0.82 +0.3 201,742 + EX28DRAFT_3537 0.85 -2.0 201,820 + +0.4 201,879 + +0.9 201,880 - +0.0 201,893 - +0.4 201,894 + -1.3 201,898 + +0.1 201,898 + -0.0 201,903 - -0.0 201,975 + -0.8 201,975 + +0.4 201,976 - +1.5 202,002 + -0.8 202,111 + EX28DRAFT_3538 0.10 +0.3 202,111 + EX28DRAFT_3538 0.10 +0.0 202,112 - EX28DRAFT_3538 0.10 +0.3 202,145 + EX28DRAFT_3538 0.17 -0.4 202,166 - EX28DRAFT_3538 0.21 +1.6 202,166 - EX28DRAFT_3538 0.21 +0.2 202,564 - -1.4 202,771 - EX28DRAFT_3539 0.12 -0.1
Or see this region's nucleotide sequence