Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3477 and EX28DRAFT_3478 are separated by 12 nucleotides EX28DRAFT_3478 and EX28DRAFT_3479 are separated by 108 nucleotides EX28DRAFT_3479 and EX28DRAFT_3480 overlap by 8 nucleotides EX28DRAFT_3480 and EX28DRAFT_3481 are separated by 684 nucleotides EX28DRAFT_3481 and EX28DRAFT_3482 overlap by 4 nucleotides
EX28DRAFT_3477: EX28DRAFT_3477 - Helix-turn-helix domain, at 143,895 to 144,509
_3477
EX28DRAFT_3478: EX28DRAFT_3478 - hypothetical protein, at 144,522 to 144,803
_3478
EX28DRAFT_3479: EX28DRAFT_3479 - hypothetical protein, at 144,912 to 145,295
_3479
EX28DRAFT_3480: EX28DRAFT_3480 - hypothetical protein, at 145,288 to 145,623
_3480
EX28DRAFT_3481: EX28DRAFT_3481 - hypothetical protein, at 146,308 to 146,532
_3481
EX28DRAFT_3482: EX28DRAFT_3482 - hypothetical protein, at 146,529 to 146,897
_3482
Position (kb) 145
146 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 144.321 kb on - strand, within EX28DRAFT_3477 at 144.340 kb on - strand, within EX28DRAFT_3477 at 144.421 kb on + strand, within EX28DRAFT_3477 at 144.421 kb on + strand, within EX28DRAFT_3477 at 144.475 kb on - strand at 144.539 kb on - strand at 144.572 kb on + strand, within EX28DRAFT_3478 at 144.614 kb on - strand, within EX28DRAFT_3478 at 144.668 kb on - strand, within EX28DRAFT_3478 at 144.704 kb on - strand, within EX28DRAFT_3478 at 144.751 kb on - strand, within EX28DRAFT_3478 at 144.840 kb on + strand at 145.084 kb on + strand, within EX28DRAFT_3479 at 145.100 kb on - strand, within EX28DRAFT_3479 at 145.123 kb on - strand, within EX28DRAFT_3479 at 145.183 kb on + strand, within EX28DRAFT_3479 at 145.183 kb on + strand, within EX28DRAFT_3479 at 145.200 kb on - strand, within EX28DRAFT_3479 at 145.215 kb on + strand, within EX28DRAFT_3479 at 145.215 kb on + strand, within EX28DRAFT_3479 at 145.215 kb on + strand, within EX28DRAFT_3479 at 145.216 kb on - strand, within EX28DRAFT_3479 at 145.217 kb on + strand, within EX28DRAFT_3479 at 145.294 kb on - strand at 145.294 kb on - strand at 145.296 kb on + strand at 145.297 kb on - strand at 145.297 kb on - strand at 145.300 kb on + strand at 145.301 kb on - strand at 145.318 kb on - strand at 145.365 kb on - strand, within EX28DRAFT_3480 at 145.382 kb on + strand, within EX28DRAFT_3480 at 145.528 kb on - strand, within EX28DRAFT_3480 at 145.561 kb on + strand, within EX28DRAFT_3480 at 145.561 kb on + strand, within EX28DRAFT_3480 at 145.561 kb on + strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.673 kb on + strand at 145.673 kb on + strand at 145.675 kb on + strand at 145.675 kb on + strand at 145.676 kb on - strand at 145.680 kb on + strand at 145.706 kb on + strand at 145.714 kb on + strand at 145.743 kb on - strand at 145.761 kb on + strand at 145.764 kb on + strand at 145.764 kb on + strand at 145.765 kb on - strand at 145.843 kb on - strand at 145.865 kb on + strand at 145.866 kb on - strand at 145.952 kb on - strand at 145.998 kb on + strand at 146.010 kb on - strand at 146.130 kb on - strand at 146.399 kb on - strand, within EX28DRAFT_3481 at 146.554 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 144,321 - EX28DRAFT_3477 0.69 +0.8 144,340 - EX28DRAFT_3477 0.72 +0.5 144,421 + EX28DRAFT_3477 0.86 -0.1 144,421 + EX28DRAFT_3477 0.86 -3.4 144,475 - +0.4 144,539 - +0.6 144,572 + EX28DRAFT_3478 0.18 -0.4 144,614 - EX28DRAFT_3478 0.33 -1.6 144,668 - EX28DRAFT_3478 0.52 -0.2 144,704 - EX28DRAFT_3478 0.65 -1.0 144,751 - EX28DRAFT_3478 0.81 -1.0 144,840 + +0.3 145,084 + EX28DRAFT_3479 0.45 -0.3 145,100 - EX28DRAFT_3479 0.49 -0.4 145,123 - EX28DRAFT_3479 0.55 +0.5 145,183 + EX28DRAFT_3479 0.71 -0.1 145,183 + EX28DRAFT_3479 0.71 -1.4 145,200 - EX28DRAFT_3479 0.75 -0.0 145,215 + EX28DRAFT_3479 0.79 -0.2 145,215 + EX28DRAFT_3479 0.79 +0.1 145,215 + EX28DRAFT_3479 0.79 -1.2 145,216 - EX28DRAFT_3479 0.79 -0.2 145,217 + EX28DRAFT_3479 0.79 -1.2 145,294 - +2.2 145,294 - +0.3 145,296 + -0.8 145,297 - -0.2 145,297 - -0.5 145,300 + +0.4 145,301 - -0.2 145,318 - +0.3 145,365 - EX28DRAFT_3480 0.23 -0.5 145,382 + EX28DRAFT_3480 0.28 +0.6 145,528 - EX28DRAFT_3480 0.71 -1.6 145,561 + EX28DRAFT_3480 0.81 +1.7 145,561 + EX28DRAFT_3480 0.81 -0.2 145,561 + EX28DRAFT_3480 0.81 -0.7 145,562 - EX28DRAFT_3480 0.82 -0.3 145,562 - EX28DRAFT_3480 0.82 -0.5 145,562 - EX28DRAFT_3480 0.82 -0.3 145,562 - EX28DRAFT_3480 0.82 +1.1 145,562 - EX28DRAFT_3480 0.82 -0.9 145,562 - EX28DRAFT_3480 0.82 -1.1 145,673 + +0.2 145,673 + -0.3 145,675 + +1.6 145,675 + +0.2 145,676 - +0.1 145,680 + +0.1 145,706 + -0.9 145,714 + -1.1 145,743 - +0.5 145,761 + +0.3 145,764 + -0.7 145,764 + -0.3 145,765 - +2.6 145,843 - -0.6 145,865 + +0.3 145,866 - +0.6 145,952 - +0.0 145,998 + +0.6 146,010 - +1.0 146,130 - +2.8 146,399 - EX28DRAFT_3481 0.40 -0.5 146,554 - -2.3
Or see this region's nucleotide sequence