Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3459 and EX28DRAFT_3460 are separated by 1 nucleotides EX28DRAFT_3460 and EX28DRAFT_3461 are separated by 264 nucleotides EX28DRAFT_3461 and EX28DRAFT_3462 are separated by 164 nucleotides EX28DRAFT_3462 and EX28DRAFT_3463 are separated by 46 nucleotides
EX28DRAFT_3459: EX28DRAFT_3459 - Protein of unknown function (DUF3828), at 133,801 to 134,253
_3459
EX28DRAFT_3460: EX28DRAFT_3460 - hypothetical protein, at 134,255 to 134,617
_3460
EX28DRAFT_3461: EX28DRAFT_3461 - ornithine carbamoyltransferase, at 134,882 to 135,886
_3461
EX28DRAFT_3462: EX28DRAFT_3462 - Uncharacterized protein conserved in bacteria, at 136,051 to 136,476
_3462
EX28DRAFT_3463: EX28DRAFT_3463 - Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA, at 136,523 to 137,281
_3463
Position (kb) 134
135
136 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 133.888 kb on + strand, within EX28DRAFT_3459 at 133.894 kb on + strand, within EX28DRAFT_3459 at 133.894 kb on + strand, within EX28DRAFT_3459 at 133.895 kb on - strand, within EX28DRAFT_3459 at 133.925 kb on + strand, within EX28DRAFT_3459 at 133.926 kb on - strand, within EX28DRAFT_3459 at 133.926 kb on - strand, within EX28DRAFT_3459 at 133.926 kb on - strand, within EX28DRAFT_3459 at 133.927 kb on + strand, within EX28DRAFT_3459 at 133.927 kb on + strand, within EX28DRAFT_3459 at 133.969 kb on + strand, within EX28DRAFT_3459 at 133.970 kb on - strand, within EX28DRAFT_3459 at 134.096 kb on + strand, within EX28DRAFT_3459 at 134.104 kb on + strand, within EX28DRAFT_3459 at 134.340 kb on + strand, within EX28DRAFT_3460 at 134.366 kb on - strand, within EX28DRAFT_3460 at 134.408 kb on - strand, within EX28DRAFT_3460 at 134.524 kb on + strand, within EX28DRAFT_3460 at 134.605 kb on + strand at 134.762 kb on - strand at 134.871 kb on - strand at 134.914 kb on + strand at 135.198 kb on + strand, within EX28DRAFT_3461 at 135.199 kb on - strand, within EX28DRAFT_3461 at 135.202 kb on + strand, within EX28DRAFT_3461 at 135.203 kb on - strand, within EX28DRAFT_3461 at 135.571 kb on - strand, within EX28DRAFT_3461 at 135.661 kb on - strand, within EX28DRAFT_3461 at 135.979 kb on + strand at 136.022 kb on - strand at 136.022 kb on - strand at 136.022 kb on - strand at 136.101 kb on + strand, within EX28DRAFT_3462 at 136.144 kb on + strand, within EX28DRAFT_3462 at 136.144 kb on + strand, within EX28DRAFT_3462 at 136.144 kb on + strand, within EX28DRAFT_3462 at 136.144 kb on + strand, within EX28DRAFT_3462 at 136.145 kb on - strand, within EX28DRAFT_3462 at 136.333 kb on - strand, within EX28DRAFT_3462 at 136.390 kb on + strand, within EX28DRAFT_3462 at 136.390 kb on + strand, within EX28DRAFT_3462 at 136.390 kb on + strand, within EX28DRAFT_3462 at 136.390 kb on + strand, within EX28DRAFT_3462 at 136.391 kb on - strand, within EX28DRAFT_3462 at 136.441 kb on + strand at 136.540 kb on - strand at 136.540 kb on - strand at 136.594 kb on + strand at 136.595 kb on - strand at 136.687 kb on + strand, within EX28DRAFT_3463 at 136.691 kb on + strand, within EX28DRAFT_3463 at 136.742 kb on + strand, within EX28DRAFT_3463 at 136.861 kb on + strand, within EX28DRAFT_3463 at 136.861 kb on + strand, within EX28DRAFT_3463 at 136.862 kb on - strand, within EX28DRAFT_3463
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 133,888 + EX28DRAFT_3459 0.19 -0.3 133,894 + EX28DRAFT_3459 0.21 +0.5 133,894 + EX28DRAFT_3459 0.21 +0.4 133,895 - EX28DRAFT_3459 0.21 +0.9 133,925 + EX28DRAFT_3459 0.27 +0.1 133,926 - EX28DRAFT_3459 0.28 +0.1 133,926 - EX28DRAFT_3459 0.28 -0.3 133,926 - EX28DRAFT_3459 0.28 +0.3 133,927 + EX28DRAFT_3459 0.28 +0.1 133,927 + EX28DRAFT_3459 0.28 -0.1 133,969 + EX28DRAFT_3459 0.37 -1.0 133,970 - EX28DRAFT_3459 0.37 +0.1 134,096 + EX28DRAFT_3459 0.65 +0.3 134,104 + EX28DRAFT_3459 0.67 +0.2 134,340 + EX28DRAFT_3460 0.23 +1.6 134,366 - EX28DRAFT_3460 0.31 +0.2 134,408 - EX28DRAFT_3460 0.42 -0.2 134,524 + EX28DRAFT_3460 0.74 +0.1 134,605 + -0.9 134,762 - -0.4 134,871 - -0.0 134,914 + -1.9 135,198 + EX28DRAFT_3461 0.31 -0.3 135,199 - EX28DRAFT_3461 0.32 -1.8 135,202 + EX28DRAFT_3461 0.32 -0.9 135,203 - EX28DRAFT_3461 0.32 -2.1 135,571 - EX28DRAFT_3461 0.69 +0.3 135,661 - EX28DRAFT_3461 0.78 -0.9 135,979 + +0.5 136,022 - -0.4 136,022 - -0.1 136,022 - +0.5 136,101 + EX28DRAFT_3462 0.12 +0.3 136,144 + EX28DRAFT_3462 0.22 +0.6 136,144 + EX28DRAFT_3462 0.22 +0.7 136,144 + EX28DRAFT_3462 0.22 -0.3 136,144 + EX28DRAFT_3462 0.22 -0.8 136,145 - EX28DRAFT_3462 0.22 -0.3 136,333 - EX28DRAFT_3462 0.66 -3.0 136,390 + EX28DRAFT_3462 0.80 +0.6 136,390 + EX28DRAFT_3462 0.80 -1.8 136,390 + EX28DRAFT_3462 0.80 +0.3 136,390 + EX28DRAFT_3462 0.80 +0.3 136,391 - EX28DRAFT_3462 0.80 +0.0 136,441 + -0.2 136,540 - +0.3 136,540 - +0.3 136,594 + -0.0 136,595 - +0.0 136,687 + EX28DRAFT_3463 0.22 +0.7 136,691 + EX28DRAFT_3463 0.22 -0.1 136,742 + EX28DRAFT_3463 0.29 -0.3 136,861 + EX28DRAFT_3463 0.45 -0.1 136,861 + EX28DRAFT_3463 0.45 -0.1 136,862 - EX28DRAFT_3463 0.45 +2.9
Or see this region's nucleotide sequence