Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3073 and EX28DRAFT_3074 are separated by 190 nucleotides EX28DRAFT_3074 and EX28DRAFT_3075 are separated by 2 nucleotides
EX28DRAFT_3073: EX28DRAFT_3073 - type I secretion outer membrane protein, TolC family, at 383,064 to 384,542
_3073
EX28DRAFT_3074: EX28DRAFT_3074 - Predicted integral membrane protein, at 384,733 to 385,401
_3074
EX28DRAFT_3075: EX28DRAFT_3075 - Glutathionylspermidine synthase, at 385,404 to 386,564
_3075
Position (kb) 384
385
386 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 383.910 kb on + strand, within EX28DRAFT_3073 at 384.039 kb on + strand, within EX28DRAFT_3073 at 384.221 kb on + strand, within EX28DRAFT_3073 at 384.221 kb on + strand, within EX28DRAFT_3073 at 384.222 kb on - strand, within EX28DRAFT_3073 at 384.258 kb on + strand, within EX28DRAFT_3073 at 384.259 kb on - strand, within EX28DRAFT_3073 at 384.444 kb on + strand at 384.447 kb on - strand at 384.525 kb on - strand at 384.537 kb on - strand at 384.540 kb on + strand at 384.639 kb on + strand at 384.639 kb on + strand at 384.705 kb on + strand at 384.750 kb on + strand at 384.829 kb on - strand, within EX28DRAFT_3074 at 384.829 kb on - strand, within EX28DRAFT_3074 at 384.829 kb on - strand, within EX28DRAFT_3074 at 384.961 kb on - strand, within EX28DRAFT_3074 at 384.961 kb on - strand, within EX28DRAFT_3074 at 384.973 kb on + strand, within EX28DRAFT_3074 at 384.973 kb on + strand, within EX28DRAFT_3074 at 385.111 kb on + strand, within EX28DRAFT_3074 at 385.112 kb on - strand, within EX28DRAFT_3074 at 385.205 kb on - strand, within EX28DRAFT_3074 at 385.206 kb on + strand, within EX28DRAFT_3074 at 385.207 kb on - strand, within EX28DRAFT_3074 at 385.466 kb on + strand at 385.482 kb on + strand at 385.515 kb on + strand at 385.515 kb on + strand at 385.515 kb on + strand at 385.516 kb on - strand at 385.516 kb on - strand at 385.695 kb on + strand, within EX28DRAFT_3075 at 385.696 kb on - strand, within EX28DRAFT_3075 at 385.751 kb on + strand, within EX28DRAFT_3075 at 385.974 kb on + strand, within EX28DRAFT_3075 at 386.022 kb on + strand, within EX28DRAFT_3075 at 386.116 kb on - strand, within EX28DRAFT_3075 at 386.116 kb on - strand, within EX28DRAFT_3075 at 386.116 kb on - strand, within EX28DRAFT_3075 at 386.116 kb on - strand, within EX28DRAFT_3075 at 386.198 kb on + strand, within EX28DRAFT_3075 at 386.262 kb on + strand, within EX28DRAFT_3075 at 386.298 kb on + strand, within EX28DRAFT_3075 at 386.299 kb on - strand, within EX28DRAFT_3075 at 386.299 kb on - strand, within EX28DRAFT_3075 at 386.299 kb on - strand, within EX28DRAFT_3075 at 386.299 kb on - strand, within EX28DRAFT_3075
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 383,910 + EX28DRAFT_3073 0.57 -0.4 384,039 + EX28DRAFT_3073 0.66 -2.7 384,221 + EX28DRAFT_3073 0.78 -2.5 384,221 + EX28DRAFT_3073 0.78 -2.8 384,222 - EX28DRAFT_3073 0.78 -1.2 384,258 + EX28DRAFT_3073 0.81 -1.0 384,259 - EX28DRAFT_3073 0.81 -0.5 384,444 + -2.0 384,447 - +0.2 384,525 - -0.5 384,537 - -0.3 384,540 + -0.4 384,639 + +0.3 384,639 + -0.8 384,705 + -1.7 384,750 + -1.1 384,829 - EX28DRAFT_3074 0.14 -0.5 384,829 - EX28DRAFT_3074 0.14 -0.6 384,829 - EX28DRAFT_3074 0.14 +0.4 384,961 - EX28DRAFT_3074 0.34 -1.3 384,961 - EX28DRAFT_3074 0.34 -1.7 384,973 + EX28DRAFT_3074 0.36 -1.2 384,973 + EX28DRAFT_3074 0.36 -0.4 385,111 + EX28DRAFT_3074 0.57 +0.1 385,112 - EX28DRAFT_3074 0.57 -0.5 385,205 - EX28DRAFT_3074 0.71 -0.1 385,206 + EX28DRAFT_3074 0.71 -0.1 385,207 - EX28DRAFT_3074 0.71 -0.4 385,466 + +0.2 385,482 + -0.5 385,515 + +0.2 385,515 + +1.7 385,515 + -1.3 385,516 - -0.6 385,516 - +2.1 385,695 + EX28DRAFT_3075 0.25 -0.6 385,696 - EX28DRAFT_3075 0.25 -0.6 385,751 + EX28DRAFT_3075 0.30 -0.7 385,974 + EX28DRAFT_3075 0.49 +0.4 386,022 + EX28DRAFT_3075 0.53 -0.6 386,116 - EX28DRAFT_3075 0.61 -0.4 386,116 - EX28DRAFT_3075 0.61 -3.1 386,116 - EX28DRAFT_3075 0.61 -0.2 386,116 - EX28DRAFT_3075 0.61 +0.0 386,198 + EX28DRAFT_3075 0.68 -3.5 386,262 + EX28DRAFT_3075 0.74 -0.1 386,298 + EX28DRAFT_3075 0.77 -0.5 386,299 - EX28DRAFT_3075 0.77 -0.4 386,299 - EX28DRAFT_3075 0.77 -0.1 386,299 - EX28DRAFT_3075 0.77 -0.9 386,299 - EX28DRAFT_3075 0.77 +0.1
Or see this region's nucleotide sequence