Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3012 and EX28DRAFT_3013 are separated by 181 nucleotides EX28DRAFT_3013 and EX28DRAFT_3014 are separated by 95 nucleotides EX28DRAFT_3014 and EX28DRAFT_3015 are separated by 139 nucleotides EX28DRAFT_3015 and EX28DRAFT_3016 are separated by 94 nucleotides
EX28DRAFT_3012: EX28DRAFT_3012 - Arginine/lysine/ornithine decarboxylases, at 320,650 to 322,785
_3012
EX28DRAFT_3013: EX28DRAFT_3013 - Uncharacterized membrane protein, possible Na+ channel or pump, at 322,967 to 323,683
_3013
EX28DRAFT_3014: EX28DRAFT_3014 - tRNA, at 323,779 to 323,854
_3014
EX28DRAFT_3015: EX28DRAFT_3015 - hypothetical protein, at 323,994 to 324,386
_3015
EX28DRAFT_3016: EX28DRAFT_3016 - Protein of unknown function (DUF1493), at 324,481 to 324,861
_3016
Position (kb)
322
323
324 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 322.057 kb on - strand, within EX28DRAFT_3012 at 322.089 kb on - strand, within EX28DRAFT_3012 at 322.232 kb on + strand, within EX28DRAFT_3012 at 322.233 kb on - strand, within EX28DRAFT_3012 at 322.233 kb on - strand, within EX28DRAFT_3012 at 322.371 kb on - strand, within EX28DRAFT_3012 at 322.371 kb on - strand, within EX28DRAFT_3012 at 322.576 kb on + strand at 322.602 kb on + strand at 322.603 kb on - strand at 322.603 kb on - strand at 322.840 kb on - strand at 322.852 kb on + strand at 322.853 kb on - strand at 322.853 kb on - strand at 322.930 kb on + strand at 322.938 kb on + strand at 322.938 kb on + strand at 322.953 kb on - strand at 322.984 kb on + strand at 322.984 kb on + strand at 322.985 kb on - strand at 323.104 kb on + strand, within EX28DRAFT_3013 at 323.112 kb on + strand, within EX28DRAFT_3013 at 323.203 kb on + strand, within EX28DRAFT_3013 at 323.204 kb on - strand, within EX28DRAFT_3013 at 323.204 kb on - strand, within EX28DRAFT_3013 at 323.281 kb on + strand, within EX28DRAFT_3013 at 323.291 kb on + strand, within EX28DRAFT_3013 at 323.599 kb on + strand, within EX28DRAFT_3013 at 323.891 kb on + strand at 323.891 kb on + strand at 323.893 kb on + strand at 323.950 kb on - strand at 323.970 kb on + strand at 323.970 kb on + strand at 323.970 kb on + strand at 323.970 kb on + strand at 324.000 kb on - strand at 324.000 kb on - strand at 324.000 kb on - strand at 324.089 kb on + strand, within EX28DRAFT_3015 at 324.089 kb on + strand, within EX28DRAFT_3015 at 324.174 kb on - strand, within EX28DRAFT_3015 at 324.174 kb on - strand, within EX28DRAFT_3015 at 324.174 kb on - strand, within EX28DRAFT_3015 at 324.182 kb on + strand, within EX28DRAFT_3015 at 324.259 kb on + strand, within EX28DRAFT_3015 at 324.259 kb on + strand, within EX28DRAFT_3015 at 324.376 kb on + strand at 324.377 kb on - strand at 324.381 kb on - strand at 324.402 kb on - strand at 324.438 kb on - strand at 324.454 kb on - strand at 324.509 kb on - strand at 324.526 kb on - strand, within EX28DRAFT_3016 at 324.528 kb on - strand, within EX28DRAFT_3016 at 324.528 kb on - strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.586 kb on - strand, within EX28DRAFT_3016 at 324.586 kb on - strand, within EX28DRAFT_3016 at 324.612 kb on - strand, within EX28DRAFT_3016 at 324.674 kb on + strand, within EX28DRAFT_3016 at 324.674 kb on + strand, within EX28DRAFT_3016
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 322,057 - EX28DRAFT_3012 0.66 +1.0 322,089 - EX28DRAFT_3012 0.67 -0.1 322,232 + EX28DRAFT_3012 0.74 -0.4 322,233 - EX28DRAFT_3012 0.74 -0.4 322,233 - EX28DRAFT_3012 0.74 -0.4 322,371 - EX28DRAFT_3012 0.81 +0.4 322,371 - EX28DRAFT_3012 0.81 +0.1 322,576 + -1.6 322,602 + +0.0 322,603 - +0.5 322,603 - +0.6 322,840 - -0.9 322,852 + +0.1 322,853 - +0.4 322,853 - +0.1 322,930 + -0.2 322,938 + -0.6 322,938 + -0.4 322,953 - -0.2 322,984 + -0.4 322,984 + +0.4 322,985 - -0.1 323,104 + EX28DRAFT_3013 0.19 +0.5 323,112 + EX28DRAFT_3013 0.20 -0.5 323,203 + EX28DRAFT_3013 0.33 +0.7 323,204 - EX28DRAFT_3013 0.33 -0.1 323,204 - EX28DRAFT_3013 0.33 -1.2 323,281 + EX28DRAFT_3013 0.44 -0.3 323,291 + EX28DRAFT_3013 0.45 -0.0 323,599 + EX28DRAFT_3013 0.88 -0.8 323,891 + +0.3 323,891 + +1.6 323,893 + -0.0 323,950 - -0.3 323,970 + +0.2 323,970 + +1.5 323,970 + +0.0 323,970 + -0.0 324,000 - +0.1 324,000 - -0.1 324,000 - +0.2 324,089 + EX28DRAFT_3015 0.24 +0.3 324,089 + EX28DRAFT_3015 0.24 -1.0 324,174 - EX28DRAFT_3015 0.46 +0.5 324,174 - EX28DRAFT_3015 0.46 +0.4 324,174 - EX28DRAFT_3015 0.46 +0.0 324,182 + EX28DRAFT_3015 0.48 -1.2 324,259 + EX28DRAFT_3015 0.67 -0.4 324,259 + EX28DRAFT_3015 0.67 -0.1 324,376 + -0.2 324,377 - +0.5 324,381 - -0.3 324,402 - -0.3 324,438 - -0.1 324,454 - +0.0 324,509 - +0.3 324,526 - EX28DRAFT_3016 0.12 +0.1 324,528 - EX28DRAFT_3016 0.12 +0.5 324,528 - EX28DRAFT_3016 0.12 -0.1 324,585 + EX28DRAFT_3016 0.27 +1.9 324,585 + EX28DRAFT_3016 0.27 -0.1 324,585 + EX28DRAFT_3016 0.27 -0.6 324,585 + EX28DRAFT_3016 0.27 -0.3 324,585 + EX28DRAFT_3016 0.27 -2.4 324,586 - EX28DRAFT_3016 0.28 +0.7 324,586 - EX28DRAFT_3016 0.28 -0.2 324,612 - EX28DRAFT_3016 0.34 -1.3 324,674 + EX28DRAFT_3016 0.51 +0.7 324,674 + EX28DRAFT_3016 0.51 -0.5
Or see this region's nucleotide sequence