Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2938 and EX28DRAFT_2939 are separated by 80 nucleotides EX28DRAFT_2939 and EX28DRAFT_2940 are separated by 163 nucleotides EX28DRAFT_2940 and EX28DRAFT_2941 are separated by 38 nucleotides
EX28DRAFT_2938: EX28DRAFT_2938 - tRNA, at 249,169 to 249,242
_2938
EX28DRAFT_2939: EX28DRAFT_2939 - Membrane proteins related to metalloendopeptidases, at 249,323 to 250,066
_2939
EX28DRAFT_2940: EX28DRAFT_2940 - isopentenyl-diphosphate delta-isomerase (EC 5.3.3.2), at 250,230 to 250,772
_2940
EX28DRAFT_2941: EX28DRAFT_2941 - Lysyl-tRNA synthetase (class II), at 250,811 to 252,328
_2941
Position (kb)
250
251 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 249.264 kb on + strand at 249.264 kb on + strand at 249.264 kb on + strand at 249.283 kb on + strand at 249.283 kb on + strand at 249.284 kb on - strand at 249.284 kb on - strand at 249.388 kb on - strand at 249.505 kb on - strand, within EX28DRAFT_2939 at 249.532 kb on + strand, within EX28DRAFT_2939 at 249.532 kb on + strand, within EX28DRAFT_2939 at 249.532 kb on + strand, within EX28DRAFT_2939 at 249.533 kb on - strand, within EX28DRAFT_2939 at 249.533 kb on - strand, within EX28DRAFT_2939 at 249.576 kb on + strand, within EX28DRAFT_2939 at 249.577 kb on - strand, within EX28DRAFT_2939 at 249.603 kb on + strand, within EX28DRAFT_2939 at 249.846 kb on + strand, within EX28DRAFT_2939 at 249.846 kb on + strand, within EX28DRAFT_2939 at 249.847 kb on - strand, within EX28DRAFT_2939 at 249.847 kb on - strand, within EX28DRAFT_2939 at 249.957 kb on + strand, within EX28DRAFT_2939 at 249.958 kb on - strand, within EX28DRAFT_2939 at 249.958 kb on - strand, within EX28DRAFT_2939 at 250.004 kb on + strand at 250.098 kb on + strand at 250.098 kb on + strand at 250.099 kb on - strand at 250.119 kb on + strand at 250.164 kb on - strand at 250.174 kb on + strand at 250.175 kb on - strand at 250.175 kb on - strand at 250.176 kb on + strand at 250.176 kb on + strand at 250.251 kb on - strand at 250.312 kb on + strand, within EX28DRAFT_2940 at 250.312 kb on + strand, within EX28DRAFT_2940 at 250.312 kb on + strand, within EX28DRAFT_2940 at 250.312 kb on + strand, within EX28DRAFT_2940 at 250.313 kb on - strand, within EX28DRAFT_2940 at 250.313 kb on - strand, within EX28DRAFT_2940 at 250.313 kb on - strand, within EX28DRAFT_2940 at 250.316 kb on + strand, within EX28DRAFT_2940 at 250.647 kb on + strand, within EX28DRAFT_2940 at 250.647 kb on + strand, within EX28DRAFT_2940 at 250.706 kb on + strand, within EX28DRAFT_2940 at 250.797 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 249,264 + -0.3 249,264 + -0.8 249,264 + +0.5 249,283 + -0.5 249,283 + +0.2 249,284 - -0.3 249,284 - -0.8 249,388 - -0.9 249,505 - EX28DRAFT_2939 0.24 +0.5 249,532 + EX28DRAFT_2939 0.28 +0.9 249,532 + EX28DRAFT_2939 0.28 +0.3 249,532 + EX28DRAFT_2939 0.28 -0.8 249,533 - EX28DRAFT_2939 0.28 +0.1 249,533 - EX28DRAFT_2939 0.28 -1.4 249,576 + EX28DRAFT_2939 0.34 +0.2 249,577 - EX28DRAFT_2939 0.34 -3.1 249,603 + EX28DRAFT_2939 0.38 -0.6 249,846 + EX28DRAFT_2939 0.70 +0.2 249,846 + EX28DRAFT_2939 0.70 +0.1 249,847 - EX28DRAFT_2939 0.70 -0.5 249,847 - EX28DRAFT_2939 0.70 -0.8 249,957 + EX28DRAFT_2939 0.85 -0.2 249,958 - EX28DRAFT_2939 0.85 -0.1 249,958 - EX28DRAFT_2939 0.85 -0.3 250,004 + +0.1 250,098 + +0.4 250,098 + -0.0 250,099 - -0.4 250,119 + -2.8 250,164 - -0.0 250,174 + -0.2 250,175 - +0.2 250,175 - +1.0 250,176 + -0.2 250,176 + +0.8 250,251 - -0.4 250,312 + EX28DRAFT_2940 0.15 +0.4 250,312 + EX28DRAFT_2940 0.15 +0.2 250,312 + EX28DRAFT_2940 0.15 +0.6 250,312 + EX28DRAFT_2940 0.15 -0.4 250,313 - EX28DRAFT_2940 0.15 -0.2 250,313 - EX28DRAFT_2940 0.15 -0.2 250,313 - EX28DRAFT_2940 0.15 +0.1 250,316 + EX28DRAFT_2940 0.16 -0.3 250,647 + EX28DRAFT_2940 0.77 -1.2 250,647 + EX28DRAFT_2940 0.77 +0.0 250,706 + EX28DRAFT_2940 0.88 -1.9 250,797 + -1.4
Or see this region's nucleotide sequence