Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2898 and EX28DRAFT_2899 overlap by 16 nucleotides EX28DRAFT_2899 and EX28DRAFT_2900 are separated by 14 nucleotides EX28DRAFT_2900 and EX28DRAFT_2901 overlap by 4 nucleotides EX28DRAFT_2901 and EX28DRAFT_2902 are separated by 184 nucleotides EX28DRAFT_2902 and EX28DRAFT_2903 are separated by 6 nucleotides
EX28DRAFT_2898: EX28DRAFT_2898 - prepilin-type N-terminal cleavage/methylation domain, at 210,187 to 210,510
_2898
EX28DRAFT_2899: EX28DRAFT_2899 - Protein of unknown function (DUF2509), at 210,495 to 210,878
_2899
EX28DRAFT_2900: EX28DRAFT_2900 - prepilin-type N-terminal cleavage/methylation domain, at 210,893 to 211,447
_2900
EX28DRAFT_2901: EX28DRAFT_2901 - prepilin-type N-terminal cleavage/methylation domain, at 211,444 to 211,908
_2901
EX28DRAFT_2902: EX28DRAFT_2902 - thymidylate synthase, at 212,093 to 212,887
_2902
EX28DRAFT_2903: EX28DRAFT_2903 - prolipoprotein diacylglyceryl transferase, at 212,894 to 213,769
_2903
Position (kb)
211
212 Strain fitness (log2 ratio)
-1
0
1 at 210.463 kb on + strand, within EX28DRAFT_2898 at 210.464 kb on - strand, within EX28DRAFT_2898 at 210.477 kb on + strand, within EX28DRAFT_2898 at 210.904 kb on - strand at 210.919 kb on - strand at 210.919 kb on - strand at 210.919 kb on - strand at 210.919 kb on - strand at 210.919 kb on - strand at 210.919 kb on - strand at 210.919 kb on - strand at 210.952 kb on + strand, within EX28DRAFT_2900 at 210.953 kb on - strand, within EX28DRAFT_2900 at 210.953 kb on - strand, within EX28DRAFT_2900 at 210.956 kb on - strand, within EX28DRAFT_2900 at 211.033 kb on + strand, within EX28DRAFT_2900 at 211.033 kb on + strand, within EX28DRAFT_2900 at 211.254 kb on + strand, within EX28DRAFT_2900 at 211.385 kb on + strand, within EX28DRAFT_2900 at 211.397 kb on - strand at 211.473 kb on + strand at 211.507 kb on - strand, within EX28DRAFT_2901 at 211.574 kb on + strand, within EX28DRAFT_2901 at 211.575 kb on - strand, within EX28DRAFT_2901 at 211.710 kb on + strand, within EX28DRAFT_2901 at 211.711 kb on - strand, within EX28DRAFT_2901 at 211.887 kb on + strand at 211.961 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 210,463 + EX28DRAFT_2898 0.85 -0.5 210,464 - EX28DRAFT_2898 0.85 +0.7 210,477 + EX28DRAFT_2898 0.90 +0.4 210,904 - -1.3 210,919 - +1.3 210,919 - -0.1 210,919 - +1.0 210,919 - +0.5 210,919 - -0.1 210,919 - -0.6 210,919 - +0.1 210,952 + EX28DRAFT_2900 0.11 -0.3 210,953 - EX28DRAFT_2900 0.11 -0.4 210,953 - EX28DRAFT_2900 0.11 +0.1 210,956 - EX28DRAFT_2900 0.11 +0.1 211,033 + EX28DRAFT_2900 0.25 -1.3 211,033 + EX28DRAFT_2900 0.25 +0.4 211,254 + EX28DRAFT_2900 0.65 -0.0 211,385 + EX28DRAFT_2900 0.89 +0.0 211,397 - +0.1 211,473 + -0.2 211,507 - EX28DRAFT_2901 0.14 -0.0 211,574 + EX28DRAFT_2901 0.28 +1.1 211,575 - EX28DRAFT_2901 0.28 -0.1 211,710 + EX28DRAFT_2901 0.57 +1.2 211,711 - EX28DRAFT_2901 0.57 -0.8 211,887 + +0.7 211,961 + -1.6
Or see this region's nucleotide sequence