Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2863 and EX28DRAFT_2864 are separated by 67 nucleotides EX28DRAFT_2864 and EX28DRAFT_2865 are separated by 51 nucleotides
EX28DRAFT_2863: EX28DRAFT_2863 - MazG family protein, at 165,976 to 166,767
_2863
EX28DRAFT_2864: EX28DRAFT_2864 - (p)ppGpp synthetase, RelA/SpoT family, at 166,835 to 169,066
_2864
EX28DRAFT_2865: EX28DRAFT_2865 - 23S rRNA (uracil-5-)-methyltransferase RumA, at 169,118 to 170,416
_2865
Position (kb)
166
167
168
169
170 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 165.874 kb on - strand at 165.902 kb on + strand at 166.330 kb on - strand, within EX28DRAFT_2863 at 166.365 kb on + strand, within EX28DRAFT_2863 at 166.530 kb on + strand, within EX28DRAFT_2863 at 166.562 kb on + strand, within EX28DRAFT_2863 at 166.563 kb on - strand, within EX28DRAFT_2863 at 166.646 kb on + strand, within EX28DRAFT_2863 at 166.663 kb on - strand, within EX28DRAFT_2863 at 166.663 kb on - strand, within EX28DRAFT_2863 at 166.920 kb on + strand at 166.936 kb on + strand at 166.937 kb on - strand at 166.937 kb on - strand at 167.111 kb on + strand, within EX28DRAFT_2864 at 167.111 kb on + strand, within EX28DRAFT_2864 at 167.111 kb on + strand, within EX28DRAFT_2864 at 167.111 kb on + strand, within EX28DRAFT_2864 at 167.197 kb on + strand, within EX28DRAFT_2864 at 167.198 kb on - strand, within EX28DRAFT_2864 at 167.317 kb on - strand, within EX28DRAFT_2864 at 168.139 kb on - strand, within EX28DRAFT_2864 at 168.182 kb on + strand, within EX28DRAFT_2864 at 168.183 kb on - strand, within EX28DRAFT_2864 at 168.298 kb on - strand, within EX28DRAFT_2864 at 168.302 kb on - strand, within EX28DRAFT_2864 at 169.080 kb on - strand at 169.120 kb on - strand at 169.260 kb on + strand, within EX28DRAFT_2865 at 169.260 kb on + strand, within EX28DRAFT_2865 at 169.260 kb on + strand, within EX28DRAFT_2865 at 169.260 kb on + strand, within EX28DRAFT_2865 at 169.261 kb on - strand, within EX28DRAFT_2865 at 169.285 kb on + strand, within EX28DRAFT_2865 at 169.288 kb on + strand, within EX28DRAFT_2865 at 169.289 kb on - strand, within EX28DRAFT_2865 at 169.289 kb on - strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.292 kb on - strand, within EX28DRAFT_2865 at 169.292 kb on - strand, within EX28DRAFT_2865 at 169.292 kb on - strand, within EX28DRAFT_2865 at 169.292 kb on - strand, within EX28DRAFT_2865 at 169.704 kb on - strand, within EX28DRAFT_2865
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 165,874 - -1.1 165,902 + +0.0 166,330 - EX28DRAFT_2863 0.45 +0.5 166,365 + EX28DRAFT_2863 0.49 +0.5 166,530 + EX28DRAFT_2863 0.70 +0.3 166,562 + EX28DRAFT_2863 0.74 +0.1 166,563 - EX28DRAFT_2863 0.74 -0.3 166,646 + EX28DRAFT_2863 0.85 +0.5 166,663 - EX28DRAFT_2863 0.87 -0.2 166,663 - EX28DRAFT_2863 0.87 +0.1 166,920 + -4.2 166,936 + -3.1 166,937 - -5.0 166,937 - -0.6 167,111 + EX28DRAFT_2864 0.12 -3.6 167,111 + EX28DRAFT_2864 0.12 -3.8 167,111 + EX28DRAFT_2864 0.12 -4.5 167,111 + EX28DRAFT_2864 0.12 -4.6 167,197 + EX28DRAFT_2864 0.16 -2.8 167,198 - EX28DRAFT_2864 0.16 -2.2 167,317 - EX28DRAFT_2864 0.22 -1.6 168,139 - EX28DRAFT_2864 0.58 -4.1 168,182 + EX28DRAFT_2864 0.60 -2.0 168,183 - EX28DRAFT_2864 0.60 -1.6 168,298 - EX28DRAFT_2864 0.66 -5.6 168,302 - EX28DRAFT_2864 0.66 -2.5 169,080 - +0.2 169,120 - -1.0 169,260 + EX28DRAFT_2865 0.11 -2.0 169,260 + EX28DRAFT_2865 0.11 +1.1 169,260 + EX28DRAFT_2865 0.11 -0.7 169,260 + EX28DRAFT_2865 0.11 -0.7 169,261 - EX28DRAFT_2865 0.11 -0.4 169,285 + EX28DRAFT_2865 0.13 -1.3 169,288 + EX28DRAFT_2865 0.13 -0.5 169,289 - EX28DRAFT_2865 0.13 -0.8 169,289 - EX28DRAFT_2865 0.13 -0.1 169,291 + EX28DRAFT_2865 0.13 -0.8 169,291 + EX28DRAFT_2865 0.13 +1.2 169,291 + EX28DRAFT_2865 0.13 +0.0 169,291 + EX28DRAFT_2865 0.13 -0.6 169,291 + EX28DRAFT_2865 0.13 -0.2 169,292 - EX28DRAFT_2865 0.13 -0.0 169,292 - EX28DRAFT_2865 0.13 +0.5 169,292 - EX28DRAFT_2865 0.13 +0.2 169,292 - EX28DRAFT_2865 0.13 +0.5 169,704 - EX28DRAFT_2865 0.45 -3.7
Or see this region's nucleotide sequence