Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2471 and EX28DRAFT_2472 overlap by 4 nucleotides EX28DRAFT_2472 and EX28DRAFT_2473 overlap by 4 nucleotides EX28DRAFT_2473 and EX28DRAFT_2474 are separated by 108 nucleotides EX28DRAFT_2474 and EX28DRAFT_2475 are separated by 50 nucleotides EX28DRAFT_2475 and EX28DRAFT_2476 overlap by 2 nucleotides
EX28DRAFT_2471: EX28DRAFT_2471 - Membrane protease subunits, stomatin/prohibitin homologs, at 2,500,249 to 2,501,163
_2471
EX28DRAFT_2472: EX28DRAFT_2472 - Membrane protein implicated in regulation of membrane protease activity, at 2,501,160 to 2,501,612
_2472
EX28DRAFT_2473: EX28DRAFT_2473 - Cu(I)-responsive transcriptional regulator, at 2,501,609 to 2,502,019
_2473
EX28DRAFT_2474: EX28DRAFT_2474 - copper-(or silver)-translocating P-type ATPase, at 2,502,128 to 2,504,626
_2474
EX28DRAFT_2475: EX28DRAFT_2475 - Uncharacterized protein conserved in bacteria, at 2,504,677 to 2,505,471
_2475
EX28DRAFT_2476: EX28DRAFT_2476 - RNA, at 2,505,470 to 2,505,548
_2476
Position (kb)
2502
2503
2504
2505 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2501.462 kb on + strand, within EX28DRAFT_2472 at 2501.890 kb on + strand, within EX28DRAFT_2473 at 2501.961 kb on + strand, within EX28DRAFT_2473 at 2502.014 kb on - strand at 2502.063 kb on + strand at 2502.064 kb on - strand at 2502.097 kb on - strand at 2502.097 kb on - strand at 2502.113 kb on + strand at 2502.114 kb on - strand at 2502.139 kb on + strand at 2502.321 kb on + strand at 2502.837 kb on - strand, within EX28DRAFT_2474 at 2502.920 kb on + strand, within EX28DRAFT_2474 at 2502.921 kb on - strand, within EX28DRAFT_2474 at 2502.921 kb on - strand, within EX28DRAFT_2474 at 2502.925 kb on + strand, within EX28DRAFT_2474 at 2502.977 kb on + strand, within EX28DRAFT_2474 at 2502.980 kb on + strand, within EX28DRAFT_2474 at 2502.981 kb on - strand, within EX28DRAFT_2474 at 2502.981 kb on - strand, within EX28DRAFT_2474 at 2503.083 kb on + strand, within EX28DRAFT_2474 at 2504.208 kb on + strand, within EX28DRAFT_2474 at 2504.371 kb on + strand, within EX28DRAFT_2474 at 2504.467 kb on + strand at 2504.468 kb on - strand at 2504.468 kb on - strand at 2504.471 kb on + strand at 2504.471 kb on + strand at 2504.471 kb on + strand at 2504.472 kb on - strand at 2504.473 kb on + strand at 2504.474 kb on - strand at 2504.474 kb on - strand at 2504.695 kb on - strand at 2504.696 kb on + strand at 2504.696 kb on + strand at 2504.814 kb on + strand, within EX28DRAFT_2475 at 2504.814 kb on + strand, within EX28DRAFT_2475 at 2504.815 kb on - strand, within EX28DRAFT_2475 at 2504.840 kb on + strand, within EX28DRAFT_2475 at 2504.841 kb on - strand, within EX28DRAFT_2475 at 2505.219 kb on + strand, within EX28DRAFT_2475 at 2505.337 kb on + strand, within EX28DRAFT_2475 at 2505.338 kb on - strand, within EX28DRAFT_2475 at 2505.338 kb on - strand, within EX28DRAFT_2475 at 2505.406 kb on + strand at 2505.406 kb on + strand at 2505.429 kb on - strand at 2505.437 kb on - strand at 2505.437 kb on - strand at 2505.484 kb on + strand, within EX28DRAFT_2476 at 2505.491 kb on - strand, within EX28DRAFT_2476 at 2505.491 kb on - strand, within EX28DRAFT_2476 at 2505.527 kb on - strand, within EX28DRAFT_2476 at 2505.527 kb on - strand, within EX28DRAFT_2476 at 2505.585 kb on + strand at 2505.588 kb on + strand at 2505.589 kb on - strand at 2505.606 kb on + strand at 2505.606 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,501,462 + EX28DRAFT_2472 0.67 -0.1 2,501,890 + EX28DRAFT_2473 0.68 +0.0 2,501,961 + EX28DRAFT_2473 0.86 -0.1 2,502,014 - -0.1 2,502,063 + +0.1 2,502,064 - +1.3 2,502,097 - -1.6 2,502,097 - -3.3 2,502,113 + +0.4 2,502,114 - -2.3 2,502,139 + -3.0 2,502,321 + -2.8 2,502,837 - EX28DRAFT_2474 0.28 -2.7 2,502,920 + EX28DRAFT_2474 0.32 -2.2 2,502,921 - EX28DRAFT_2474 0.32 -1.7 2,502,921 - EX28DRAFT_2474 0.32 -0.9 2,502,925 + EX28DRAFT_2474 0.32 -5.3 2,502,977 + EX28DRAFT_2474 0.34 -2.7 2,502,980 + EX28DRAFT_2474 0.34 -3.5 2,502,981 - EX28DRAFT_2474 0.34 -1.7 2,502,981 - EX28DRAFT_2474 0.34 -4.1 2,503,083 + EX28DRAFT_2474 0.38 -1.2 2,504,208 + EX28DRAFT_2474 0.83 -3.4 2,504,371 + EX28DRAFT_2474 0.90 -1.1 2,504,467 + -2.4 2,504,468 - -3.2 2,504,468 - -3.0 2,504,471 + -1.7 2,504,471 + -1.9 2,504,471 + -2.5 2,504,472 - -5.1 2,504,473 + -4.4 2,504,474 - -1.7 2,504,474 - -3.1 2,504,695 - -0.5 2,504,696 + -0.5 2,504,696 + -0.9 2,504,814 + EX28DRAFT_2475 0.17 +0.7 2,504,814 + EX28DRAFT_2475 0.17 -0.2 2,504,815 - EX28DRAFT_2475 0.17 +0.1 2,504,840 + EX28DRAFT_2475 0.21 +0.1 2,504,841 - EX28DRAFT_2475 0.21 -3.4 2,505,219 + EX28DRAFT_2475 0.68 +0.8 2,505,337 + EX28DRAFT_2475 0.83 -1.0 2,505,338 - EX28DRAFT_2475 0.83 -0.4 2,505,338 - EX28DRAFT_2475 0.83 -0.7 2,505,406 + -0.0 2,505,406 + -0.3 2,505,429 - +1.8 2,505,437 - -0.7 2,505,437 - -2.5 2,505,484 + EX28DRAFT_2476 0.18 +0.1 2,505,491 - EX28DRAFT_2476 0.27 +0.4 2,505,491 - EX28DRAFT_2476 0.27 +0.2 2,505,527 - EX28DRAFT_2476 0.72 -0.1 2,505,527 - EX28DRAFT_2476 0.72 -1.0 2,505,585 + -1.8 2,505,588 + -0.1 2,505,589 - -0.1 2,505,606 + +0.9 2,505,606 + -0.7
Or see this region's nucleotide sequence