Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2457 and EX28DRAFT_2458 are separated by 68 nucleotides EX28DRAFT_2458 and EX28DRAFT_2459 are separated by 161 nucleotides EX28DRAFT_2459 and EX28DRAFT_2460 overlap by 4 nucleotides EX28DRAFT_2460 and EX28DRAFT_2461 are separated by 84 nucleotides
EX28DRAFT_2457: EX28DRAFT_2457 - Protein of unknown function (DUF1471), at 2,487,499 to 2,487,708
_2457
EX28DRAFT_2458: EX28DRAFT_2458 - hypothetical protein, at 2,487,777 to 2,487,962
_2458
EX28DRAFT_2459: EX28DRAFT_2459 - phosphoribosylaminoimidazole carboxylase, PurE protein, at 2,488,124 to 2,488,633
_2459
EX28DRAFT_2460: EX28DRAFT_2460 - phosphoribosylaminoimidazole carboxylase, PurK protein, at 2,488,630 to 2,489,697
_2460
EX28DRAFT_2461: EX28DRAFT_2461 - tRNA 2-selenouridine synthase, at 2,489,782 to 2,490,852
_2461
Position (kb)
2488
2489
2490 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2487.647 kb on - strand, within EX28DRAFT_2457 at 2487.648 kb on + strand, within EX28DRAFT_2457 at 2487.649 kb on - strand, within EX28DRAFT_2457 at 2487.682 kb on + strand, within EX28DRAFT_2457 at 2487.682 kb on + strand, within EX28DRAFT_2457 at 2487.683 kb on - strand, within EX28DRAFT_2457 at 2487.707 kb on - strand at 2487.735 kb on - strand at 2487.755 kb on + strand at 2487.796 kb on + strand, within EX28DRAFT_2458 at 2487.796 kb on + strand, within EX28DRAFT_2458 at 2487.796 kb on + strand, within EX28DRAFT_2458 at 2487.796 kb on + strand, within EX28DRAFT_2458 at 2487.797 kb on - strand, within EX28DRAFT_2458 at 2487.801 kb on - strand, within EX28DRAFT_2458 at 2487.960 kb on + strand at 2487.970 kb on + strand at 2487.970 kb on + strand at 2488.018 kb on - strand at 2488.061 kb on + strand at 2488.555 kb on - strand, within EX28DRAFT_2459 at 2488.664 kb on + strand at 2489.254 kb on - strand, within EX28DRAFT_2460 at 2489.284 kb on + strand, within EX28DRAFT_2460 at 2489.569 kb on - strand, within EX28DRAFT_2460 at 2489.695 kb on + strand at 2489.696 kb on - strand at 2489.738 kb on + strand at 2489.738 kb on + strand at 2489.739 kb on - strand at 2489.935 kb on + strand, within EX28DRAFT_2461 at 2489.935 kb on + strand, within EX28DRAFT_2461 at 2489.935 kb on + strand, within EX28DRAFT_2461 at 2490.087 kb on + strand, within EX28DRAFT_2461 at 2490.088 kb on - strand, within EX28DRAFT_2461 at 2490.148 kb on - strand, within EX28DRAFT_2461 at 2490.148 kb on - strand, within EX28DRAFT_2461 at 2490.529 kb on + strand, within EX28DRAFT_2461 at 2490.601 kb on + strand, within EX28DRAFT_2461
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,487,647 - EX28DRAFT_2457 0.70 -3.2 2,487,648 + EX28DRAFT_2457 0.71 -1.1 2,487,649 - EX28DRAFT_2457 0.71 +0.9 2,487,682 + EX28DRAFT_2457 0.87 +0.1 2,487,682 + EX28DRAFT_2457 0.87 +0.5 2,487,683 - EX28DRAFT_2457 0.88 -0.9 2,487,707 - +0.9 2,487,735 - -0.0 2,487,755 + +0.2 2,487,796 + EX28DRAFT_2458 0.10 +0.2 2,487,796 + EX28DRAFT_2458 0.10 +0.9 2,487,796 + EX28DRAFT_2458 0.10 +1.8 2,487,796 + EX28DRAFT_2458 0.10 +0.6 2,487,797 - EX28DRAFT_2458 0.11 +0.3 2,487,801 - EX28DRAFT_2458 0.13 +0.2 2,487,960 + -0.4 2,487,970 + +0.2 2,487,970 + +0.3 2,488,018 - -0.4 2,488,061 + -0.7 2,488,555 - EX28DRAFT_2459 0.85 +2.2 2,488,664 + -1.0 2,489,254 - EX28DRAFT_2460 0.58 +4.2 2,489,284 + EX28DRAFT_2460 0.61 +2.2 2,489,569 - EX28DRAFT_2460 0.88 -0.2 2,489,695 + +0.0 2,489,696 - +0.1 2,489,738 + +1.2 2,489,738 + -0.6 2,489,739 - -1.1 2,489,935 + EX28DRAFT_2461 0.14 -0.2 2,489,935 + EX28DRAFT_2461 0.14 +0.4 2,489,935 + EX28DRAFT_2461 0.14 -0.4 2,490,087 + EX28DRAFT_2461 0.28 -0.7 2,490,088 - EX28DRAFT_2461 0.29 -0.2 2,490,148 - EX28DRAFT_2461 0.34 -0.2 2,490,148 - EX28DRAFT_2461 0.34 +0.2 2,490,529 + EX28DRAFT_2461 0.70 -1.0 2,490,601 + EX28DRAFT_2461 0.76 -0.1
Or see this region's nucleotide sequence