Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2299 and EX28DRAFT_2300 are separated by 45 nucleotides EX28DRAFT_2300 and EX28DRAFT_2301 are separated by 249 nucleotides
EX28DRAFT_2299: EX28DRAFT_2299 - Transcriptional regulator/sugar kinase, at 2,328,784 to 2,330,004
_2299
EX28DRAFT_2300: EX28DRAFT_2300 - Predicted sugar phosphatases of the HAD superfamily, at 2,330,050 to 2,330,802
_2300
EX28DRAFT_2301: EX28DRAFT_2301 - asparagine synthase (glutamine-hydrolyzing), at 2,331,052 to 2,332,716
_2301
Position (kb)
2330
2331 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2329.104 kb on + strand, within EX28DRAFT_2299 at 2329.104 kb on + strand, within EX28DRAFT_2299 at 2329.127 kb on - strand, within EX28DRAFT_2299 at 2329.177 kb on - strand, within EX28DRAFT_2299 at 2329.192 kb on - strand, within EX28DRAFT_2299 at 2329.285 kb on - strand, within EX28DRAFT_2299 at 2329.342 kb on - strand, within EX28DRAFT_2299 at 2329.397 kb on - strand, within EX28DRAFT_2299 at 2329.397 kb on - strand, within EX28DRAFT_2299 at 2329.465 kb on - strand, within EX28DRAFT_2299 at 2329.467 kb on + strand, within EX28DRAFT_2299 at 2329.468 kb on - strand, within EX28DRAFT_2299 at 2329.674 kb on - strand, within EX28DRAFT_2299 at 2329.736 kb on + strand, within EX28DRAFT_2299 at 2329.737 kb on - strand, within EX28DRAFT_2299 at 2330.013 kb on + strand at 2330.013 kb on + strand at 2330.015 kb on + strand at 2330.015 kb on + strand at 2330.016 kb on - strand at 2330.016 kb on - strand at 2330.016 kb on - strand at 2330.016 kb on - strand at 2330.019 kb on - strand at 2330.059 kb on - strand at 2330.066 kb on + strand at 2330.066 kb on + strand at 2330.133 kb on - strand, within EX28DRAFT_2300 at 2330.179 kb on + strand, within EX28DRAFT_2300 at 2330.180 kb on - strand, within EX28DRAFT_2300 at 2330.254 kb on + strand, within EX28DRAFT_2300 at 2330.255 kb on - strand, within EX28DRAFT_2300 at 2330.255 kb on - strand, within EX28DRAFT_2300 at 2330.416 kb on - strand, within EX28DRAFT_2300 at 2330.443 kb on + strand, within EX28DRAFT_2300 at 2330.444 kb on - strand, within EX28DRAFT_2300 at 2330.837 kb on + strand at 2330.839 kb on + strand at 2331.009 kb on - strand at 2331.009 kb on - strand at 2331.023 kb on - strand at 2331.023 kb on - strand at 2331.051 kb on + strand at 2331.051 kb on + strand at 2331.051 kb on + strand at 2331.051 kb on + strand at 2331.052 kb on - strand at 2331.052 kb on - strand at 2331.077 kb on - strand at 2331.082 kb on - strand at 2331.106 kb on - strand at 2331.207 kb on + strand at 2331.208 kb on - strand at 2331.241 kb on + strand, within EX28DRAFT_2301 at 2331.242 kb on - strand, within EX28DRAFT_2301 at 2331.242 kb on - strand, within EX28DRAFT_2301 at 2331.242 kb on - strand, within EX28DRAFT_2301 at 2331.287 kb on - strand, within EX28DRAFT_2301 at 2331.287 kb on - strand, within EX28DRAFT_2301 at 2331.287 kb on - strand, within EX28DRAFT_2301 at 2331.371 kb on - strand, within EX28DRAFT_2301 at 2331.452 kb on - strand, within EX28DRAFT_2301 at 2331.490 kb on + strand, within EX28DRAFT_2301 at 2331.491 kb on - strand, within EX28DRAFT_2301 at 2331.491 kb on - strand, within EX28DRAFT_2301 at 2331.491 kb on - strand, within EX28DRAFT_2301 at 2331.491 kb on - strand, within EX28DRAFT_2301 at 2331.520 kb on + strand, within EX28DRAFT_2301 at 2331.622 kb on + strand, within EX28DRAFT_2301 at 2331.622 kb on + strand, within EX28DRAFT_2301 at 2331.623 kb on - strand, within EX28DRAFT_2301 at 2331.623 kb on - strand, within EX28DRAFT_2301 at 2331.647 kb on - strand, within EX28DRAFT_2301
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,329,104 + EX28DRAFT_2299 0.26 -1.3 2,329,104 + EX28DRAFT_2299 0.26 -1.0 2,329,127 - EX28DRAFT_2299 0.28 -1.7 2,329,177 - EX28DRAFT_2299 0.32 -2.5 2,329,192 - EX28DRAFT_2299 0.33 -3.7 2,329,285 - EX28DRAFT_2299 0.41 -1.2 2,329,342 - EX28DRAFT_2299 0.46 +0.7 2,329,397 - EX28DRAFT_2299 0.50 -1.1 2,329,397 - EX28DRAFT_2299 0.50 -2.6 2,329,465 - EX28DRAFT_2299 0.56 -2.6 2,329,467 + EX28DRAFT_2299 0.56 -1.4 2,329,468 - EX28DRAFT_2299 0.56 -1.3 2,329,674 - EX28DRAFT_2299 0.73 -1.7 2,329,736 + EX28DRAFT_2299 0.78 -1.9 2,329,737 - EX28DRAFT_2299 0.78 -1.7 2,330,013 + -1.2 2,330,013 + +0.2 2,330,015 + -0.3 2,330,015 + -0.2 2,330,016 - +0.0 2,330,016 - -0.2 2,330,016 - -0.7 2,330,016 - -0.7 2,330,019 - -0.4 2,330,059 - -0.7 2,330,066 + -0.5 2,330,066 + -0.0 2,330,133 - EX28DRAFT_2300 0.11 -0.5 2,330,179 + EX28DRAFT_2300 0.17 -0.5 2,330,180 - EX28DRAFT_2300 0.17 -0.3 2,330,254 + EX28DRAFT_2300 0.27 -0.4 2,330,255 - EX28DRAFT_2300 0.27 -0.0 2,330,255 - EX28DRAFT_2300 0.27 -0.2 2,330,416 - EX28DRAFT_2300 0.49 -3.0 2,330,443 + EX28DRAFT_2300 0.52 -0.4 2,330,444 - EX28DRAFT_2300 0.52 -0.1 2,330,837 + -0.4 2,330,839 + +0.9 2,331,009 - -0.5 2,331,009 - +0.2 2,331,023 - -0.2 2,331,023 - -1.0 2,331,051 + +0.2 2,331,051 + +3.7 2,331,051 + +1.7 2,331,051 + -0.9 2,331,052 - +0.1 2,331,052 - -0.3 2,331,077 - -0.3 2,331,082 - -0.7 2,331,106 - +0.2 2,331,207 + +0.0 2,331,208 - +0.1 2,331,241 + EX28DRAFT_2301 0.11 +1.3 2,331,242 - EX28DRAFT_2301 0.11 -0.0 2,331,242 - EX28DRAFT_2301 0.11 +0.1 2,331,242 - EX28DRAFT_2301 0.11 -0.2 2,331,287 - EX28DRAFT_2301 0.14 +0.4 2,331,287 - EX28DRAFT_2301 0.14 +0.7 2,331,287 - EX28DRAFT_2301 0.14 +0.1 2,331,371 - EX28DRAFT_2301 0.19 +0.7 2,331,452 - EX28DRAFT_2301 0.24 -0.5 2,331,490 + EX28DRAFT_2301 0.26 +1.7 2,331,491 - EX28DRAFT_2301 0.26 +0.7 2,331,491 - EX28DRAFT_2301 0.26 -0.9 2,331,491 - EX28DRAFT_2301 0.26 +0.4 2,331,491 - EX28DRAFT_2301 0.26 +0.2 2,331,520 + EX28DRAFT_2301 0.28 +0.4 2,331,622 + EX28DRAFT_2301 0.34 +0.6 2,331,622 + EX28DRAFT_2301 0.34 +0.6 2,331,623 - EX28DRAFT_2301 0.34 +0.0 2,331,623 - EX28DRAFT_2301 0.34 +0.6 2,331,647 - EX28DRAFT_2301 0.36 +1.7
Or see this region's nucleotide sequence