Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2216 and EX28DRAFT_2217 are separated by 68 nucleotides EX28DRAFT_2217 and EX28DRAFT_2218 are separated by 48 nucleotides EX28DRAFT_2218 and EX28DRAFT_2219 are separated by 103 nucleotides
EX28DRAFT_2216: EX28DRAFT_2216 - biotin synthase (EC 2.8.1.6), at 2,239,159 to 2,240,199
_2216
EX28DRAFT_2217: EX28DRAFT_2217 - adenosylmethionine-8-amino-7-oxononanoate transaminase, at 2,240,268 to 2,241,575
_2217
EX28DRAFT_2218: EX28DRAFT_2218 - Raf kinase inhibitor-like protein, YbhB/YbcL family, at 2,241,624 to 2,242,100
_2218
EX28DRAFT_2219: EX28DRAFT_2219 - histidine ammonia-lyase (EC 4.3.1.3), at 2,242,204 to 2,243,724
_2219
Position (kb)
2240
2241
2242 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2239.344 kb on - strand, within EX28DRAFT_2216 at 2239.751 kb on + strand, within EX28DRAFT_2216 at 2239.751 kb on + strand, within EX28DRAFT_2216 at 2239.751 kb on + strand, within EX28DRAFT_2216 at 2239.752 kb on - strand, within EX28DRAFT_2216 at 2239.752 kb on - strand, within EX28DRAFT_2216 at 2239.755 kb on - strand, within EX28DRAFT_2216 at 2239.815 kb on + strand, within EX28DRAFT_2216 at 2239.816 kb on - strand, within EX28DRAFT_2216 at 2239.816 kb on - strand, within EX28DRAFT_2216 at 2239.865 kb on + strand, within EX28DRAFT_2216 at 2240.024 kb on + strand, within EX28DRAFT_2216 at 2240.223 kb on + strand at 2240.242 kb on + strand at 2240.321 kb on + strand at 2240.321 kb on + strand at 2240.322 kb on - strand at 2240.334 kb on + strand at 2240.334 kb on + strand at 2240.334 kb on + strand at 2240.335 kb on - strand at 2240.461 kb on - strand, within EX28DRAFT_2217 at 2240.557 kb on + strand, within EX28DRAFT_2217 at 2240.557 kb on + strand, within EX28DRAFT_2217 at 2240.662 kb on - strand, within EX28DRAFT_2217 at 2240.716 kb on - strand, within EX28DRAFT_2217 at 2240.716 kb on - strand, within EX28DRAFT_2217 at 2240.953 kb on - strand, within EX28DRAFT_2217 at 2241.197 kb on + strand, within EX28DRAFT_2217 at 2241.198 kb on - strand, within EX28DRAFT_2217 at 2241.215 kb on + strand, within EX28DRAFT_2217 at 2241.473 kb on + strand at 2241.473 kb on + strand at 2241.474 kb on - strand at 2241.474 kb on - strand at 2241.474 kb on - strand at 2241.474 kb on - strand at 2241.478 kb on - strand at 2241.478 kb on - strand at 2241.500 kb on + strand at 2241.573 kb on + strand at 2241.573 kb on + strand at 2241.573 kb on + strand at 2241.573 kb on + strand at 2241.573 kb on + strand at 2241.573 kb on + strand at 2241.574 kb on - strand at 2241.580 kb on - strand at 2241.586 kb on + strand at 2241.587 kb on - strand at 2241.587 kb on - strand at 2241.710 kb on + strand, within EX28DRAFT_2218 at 2241.710 kb on + strand, within EX28DRAFT_2218 at 2241.766 kb on + strand, within EX28DRAFT_2218 at 2241.921 kb on - strand, within EX28DRAFT_2218 at 2241.969 kb on + strand, within EX28DRAFT_2218 at 2241.970 kb on - strand, within EX28DRAFT_2218 at 2241.971 kb on + strand, within EX28DRAFT_2218 at 2241.971 kb on + strand, within EX28DRAFT_2218 at 2241.971 kb on + strand, within EX28DRAFT_2218 at 2242.046 kb on + strand, within EX28DRAFT_2218 at 2242.046 kb on + strand, within EX28DRAFT_2218 at 2242.092 kb on + strand at 2242.092 kb on + strand at 2242.093 kb on - strand at 2242.093 kb on - strand at 2242.098 kb on + strand at 2242.099 kb on - strand at 2242.099 kb on - strand at 2242.102 kb on - strand at 2242.219 kb on + strand at 2242.220 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,239,344 - EX28DRAFT_2216 0.18 -2.5 2,239,751 + EX28DRAFT_2216 0.57 -2.9 2,239,751 + EX28DRAFT_2216 0.57 -0.9 2,239,751 + EX28DRAFT_2216 0.57 -2.0 2,239,752 - EX28DRAFT_2216 0.57 -0.2 2,239,752 - EX28DRAFT_2216 0.57 -4.1 2,239,755 - EX28DRAFT_2216 0.57 -1.3 2,239,815 + EX28DRAFT_2216 0.63 -0.9 2,239,816 - EX28DRAFT_2216 0.63 -3.2 2,239,816 - EX28DRAFT_2216 0.63 -3.5 2,239,865 + EX28DRAFT_2216 0.68 -3.4 2,240,024 + EX28DRAFT_2216 0.83 -3.8 2,240,223 + -5.0 2,240,242 + -2.6 2,240,321 + -2.0 2,240,321 + -1.7 2,240,322 - -1.6 2,240,334 + -2.3 2,240,334 + -1.1 2,240,334 + -3.7 2,240,335 - -1.1 2,240,461 - EX28DRAFT_2217 0.15 -0.7 2,240,557 + EX28DRAFT_2217 0.22 -2.3 2,240,557 + EX28DRAFT_2217 0.22 -0.9 2,240,662 - EX28DRAFT_2217 0.30 -4.4 2,240,716 - EX28DRAFT_2217 0.34 -1.5 2,240,716 - EX28DRAFT_2217 0.34 -1.6 2,240,953 - EX28DRAFT_2217 0.52 -1.5 2,241,197 + EX28DRAFT_2217 0.71 -0.9 2,241,198 - EX28DRAFT_2217 0.71 -1.9 2,241,215 + EX28DRAFT_2217 0.72 -1.9 2,241,473 + -3.1 2,241,473 + -0.6 2,241,474 - -0.8 2,241,474 - -1.5 2,241,474 - -0.7 2,241,474 - -1.6 2,241,478 - -1.2 2,241,478 - -1.2 2,241,500 + -2.2 2,241,573 + -0.9 2,241,573 + -0.5 2,241,573 + +0.4 2,241,573 + -0.1 2,241,573 + +0.2 2,241,573 + +0.0 2,241,574 - +1.1 2,241,580 - +0.1 2,241,586 + -0.5 2,241,587 - +0.6 2,241,587 - +2.7 2,241,710 + EX28DRAFT_2218 0.18 -2.0 2,241,710 + EX28DRAFT_2218 0.18 -0.7 2,241,766 + EX28DRAFT_2218 0.30 +0.7 2,241,921 - EX28DRAFT_2218 0.62 -0.5 2,241,969 + EX28DRAFT_2218 0.72 +0.3 2,241,970 - EX28DRAFT_2218 0.73 +0.9 2,241,971 + EX28DRAFT_2218 0.73 -1.8 2,241,971 + EX28DRAFT_2218 0.73 -0.0 2,241,971 + EX28DRAFT_2218 0.73 -2.3 2,242,046 + EX28DRAFT_2218 0.88 +0.4 2,242,046 + EX28DRAFT_2218 0.88 -2.6 2,242,092 + -2.6 2,242,092 + +1.2 2,242,093 - -1.2 2,242,093 - +0.0 2,242,098 + +0.3 2,242,099 - +0.3 2,242,099 - +0.4 2,242,102 - -0.1 2,242,219 + -0.0 2,242,220 - +0.1
Or see this region's nucleotide sequence