Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2025 and EX28DRAFT_2026 are separated by 111 nucleotides EX28DRAFT_2026 and EX28DRAFT_2027 are separated by 185 nucleotides EX28DRAFT_2027 and EX28DRAFT_2028 are separated by 69 nucleotides
EX28DRAFT_2025: EX28DRAFT_2025 - ribosomal protein S1, at 2,036,456 to 2,038,129
_2025
EX28DRAFT_2026: EX28DRAFT_2026 - cytidylate kinase, at 2,038,241 to 2,038,924
_2026
EX28DRAFT_2027: EX28DRAFT_2027 - 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19), at 2,039,110 to 2,040,393
_2027
EX28DRAFT_2028: EX28DRAFT_2028 - phosphoserine aminotransferase apoenzyme (EC 2.6.1.52), at 2,040,463 to 2,041,515
_2028
Position (kb)
2039
2040
2041 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2038.937 kb on - strand at 2038.937 kb on - strand at 2038.941 kb on - strand at 2038.941 kb on - strand at 2038.974 kb on + strand at 2038.975 kb on - strand at 2038.975 kb on - strand at 2038.997 kb on + strand at 2039.015 kb on - strand at 2039.038 kb on - strand at 2039.103 kb on + strand at 2039.103 kb on + strand at 2039.104 kb on - strand at 2039.122 kb on - strand at 2039.122 kb on - strand at 2039.124 kb on + strand at 2039.125 kb on - strand at 2039.236 kb on - strand at 2039.301 kb on - strand, within EX28DRAFT_2027 at 2039.390 kb on + strand, within EX28DRAFT_2027 at 2039.390 kb on + strand, within EX28DRAFT_2027 at 2039.390 kb on + strand, within EX28DRAFT_2027 at 2039.614 kb on + strand, within EX28DRAFT_2027 at 2039.795 kb on + strand, within EX28DRAFT_2027 at 2039.835 kb on + strand, within EX28DRAFT_2027 at 2039.922 kb on + strand, within EX28DRAFT_2027 at 2040.376 kb on + strand at 2040.418 kb on + strand at 2040.419 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,038,937 - -0.0 2,038,937 - -1.4 2,038,941 - +0.1 2,038,941 - -0.8 2,038,974 + -1.1 2,038,975 - +0.3 2,038,975 - -0.3 2,038,997 + +1.0 2,039,015 - -0.2 2,039,038 - -0.5 2,039,103 + -0.5 2,039,103 + +0.6 2,039,104 - -0.4 2,039,122 - -1.2 2,039,122 - -0.3 2,039,124 + -0.4 2,039,125 - -0.3 2,039,236 - -3.6 2,039,301 - EX28DRAFT_2027 0.15 +0.5 2,039,390 + EX28DRAFT_2027 0.22 -1.1 2,039,390 + EX28DRAFT_2027 0.22 +1.1 2,039,390 + EX28DRAFT_2027 0.22 -1.5 2,039,614 + EX28DRAFT_2027 0.39 +0.6 2,039,795 + EX28DRAFT_2027 0.53 -2.4 2,039,835 + EX28DRAFT_2027 0.56 -2.8 2,039,922 + EX28DRAFT_2027 0.63 -2.7 2,040,376 + -1.1 2,040,418 + -1.7 2,040,419 - -2.8
Or see this region's nucleotide sequence