Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2015 and EX28DRAFT_2016 are separated by 124 nucleotides EX28DRAFT_2016 and EX28DRAFT_2017 overlap by 24 nucleotides EX28DRAFT_2017 and EX28DRAFT_2018 are separated by 141 nucleotides EX28DRAFT_2018 and EX28DRAFT_2019 overlap by 4 nucleotides
EX28DRAFT_2015: EX28DRAFT_2015 - 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase, at 2,025,817 to 2,026,596
_2015
EX28DRAFT_2016: EX28DRAFT_2016 - Uncharacterized conserved protein, at 2,026,721 to 2,027,500
_2016
EX28DRAFT_2017: EX28DRAFT_2017 - Phosphotransferase enzyme family, at 2,027,477 to 2,028,370
_2017
EX28DRAFT_2018: EX28DRAFT_2018 - 3-deoxy-D-manno-octulosonate cytidylyltransferase, at 2,028,512 to 2,029,258
_2018
EX28DRAFT_2019: EX28DRAFT_2019 - Uncharacterized conserved protein, at 2,029,255 to 2,029,437
_2019
Position (kb)
2027
2028
2029 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2026.670 kb on + strand at 2026.670 kb on + strand at 2026.670 kb on + strand at 2026.671 kb on - strand at 2026.674 kb on - strand at 2026.674 kb on - strand at 2026.683 kb on - strand at 2026.683 kb on - strand at 2026.693 kb on + strand at 2026.693 kb on + strand at 2026.693 kb on + strand at 2026.693 kb on + strand at 2026.694 kb on - strand at 2026.694 kb on - strand at 2026.695 kb on + strand at 2026.695 kb on + strand at 2026.696 kb on - strand at 2026.696 kb on - strand at 2026.736 kb on - strand at 2026.742 kb on + strand at 2026.743 kb on - strand at 2026.743 kb on - strand at 2026.846 kb on + strand, within EX28DRAFT_2016 at 2026.849 kb on + strand, within EX28DRAFT_2016 at 2026.867 kb on + strand, within EX28DRAFT_2016 at 2026.909 kb on - strand, within EX28DRAFT_2016 at 2026.961 kb on + strand, within EX28DRAFT_2016 at 2026.962 kb on - strand, within EX28DRAFT_2016 at 2026.985 kb on + strand, within EX28DRAFT_2016 at 2026.985 kb on + strand, within EX28DRAFT_2016 at 2026.987 kb on + strand, within EX28DRAFT_2016 at 2026.987 kb on + strand, within EX28DRAFT_2016 at 2026.987 kb on + strand, within EX28DRAFT_2016 at 2026.987 kb on + strand, within EX28DRAFT_2016 at 2026.988 kb on - strand, within EX28DRAFT_2016 at 2027.212 kb on + strand, within EX28DRAFT_2016 at 2027.419 kb on + strand, within EX28DRAFT_2016 at 2027.578 kb on - strand, within EX28DRAFT_2017 at 2027.628 kb on + strand, within EX28DRAFT_2017 at 2027.639 kb on + strand, within EX28DRAFT_2017 at 2027.640 kb on - strand, within EX28DRAFT_2017 at 2027.777 kb on - strand, within EX28DRAFT_2017 at 2027.851 kb on + strand, within EX28DRAFT_2017 at 2027.851 kb on + strand, within EX28DRAFT_2017 at 2027.851 kb on + strand, within EX28DRAFT_2017 at 2027.852 kb on - strand, within EX28DRAFT_2017 at 2028.270 kb on + strand, within EX28DRAFT_2017 at 2028.271 kb on - strand, within EX28DRAFT_2017 at 2028.271 kb on - strand, within EX28DRAFT_2017 at 2028.324 kb on - strand at 2028.371 kb on - strand at 2028.379 kb on - strand at 2028.402 kb on + strand at 2028.403 kb on - strand at 2028.403 kb on - strand at 2028.405 kb on - strand at 2028.468 kb on - strand at 2028.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 2,026,670 + +0.8 2,026,670 + -0.7 2,026,670 + -0.3 2,026,671 - +0.1 2,026,674 - -0.1 2,026,674 - -1.5 2,026,683 - -0.1 2,026,683 - +0.4 2,026,693 + -2.9 2,026,693 + -1.2 2,026,693 + +0.2 2,026,693 + +0.6 2,026,694 - +0.1 2,026,694 - -1.3 2,026,695 + +0.2 2,026,695 + +0.5 2,026,696 - -0.0 2,026,696 - -0.7 2,026,736 - +0.3 2,026,742 + +0.0 2,026,743 - +0.1 2,026,743 - -0.5 2,026,846 + EX28DRAFT_2016 0.16 +0.1 2,026,849 + EX28DRAFT_2016 0.16 +0.4 2,026,867 + EX28DRAFT_2016 0.19 -0.7 2,026,909 - EX28DRAFT_2016 0.24 -1.0 2,026,961 + EX28DRAFT_2016 0.31 +0.1 2,026,962 - EX28DRAFT_2016 0.31 -1.5 2,026,985 + EX28DRAFT_2016 0.34 +0.2 2,026,985 + EX28DRAFT_2016 0.34 +0.7 2,026,987 + EX28DRAFT_2016 0.34 +0.0 2,026,987 + EX28DRAFT_2016 0.34 +0.5 2,026,987 + EX28DRAFT_2016 0.34 -0.5 2,026,987 + EX28DRAFT_2016 0.34 +0.2 2,026,988 - EX28DRAFT_2016 0.34 +0.9 2,027,212 + EX28DRAFT_2016 0.63 -0.0 2,027,419 + EX28DRAFT_2016 0.89 -0.5 2,027,578 - EX28DRAFT_2017 0.11 +0.0 2,027,628 + EX28DRAFT_2017 0.17 -1.6 2,027,639 + EX28DRAFT_2017 0.18 -0.6 2,027,640 - EX28DRAFT_2017 0.18 -1.2 2,027,777 - EX28DRAFT_2017 0.34 -0.3 2,027,851 + EX28DRAFT_2017 0.42 -2.3 2,027,851 + EX28DRAFT_2017 0.42 +1.6 2,027,851 + EX28DRAFT_2017 0.42 +0.2 2,027,852 - EX28DRAFT_2017 0.42 +1.1 2,028,270 + EX28DRAFT_2017 0.89 -1.2 2,028,271 - EX28DRAFT_2017 0.89 -0.8 2,028,271 - EX28DRAFT_2017 0.89 -0.3 2,028,324 - -0.4 2,028,371 - +0.1 2,028,379 - -0.6 2,028,402 + -0.3 2,028,403 - +0.5 2,028,403 - +0.7 2,028,405 - -0.1 2,028,468 - -0.3 2,028,514 - -0.0
Or see this region's nucleotide sequence