Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1977 and EX28DRAFT_1978 are separated by 35 nucleotides EX28DRAFT_1978 and EX28DRAFT_1979 are separated by 173 nucleotides EX28DRAFT_1979 and EX28DRAFT_1980 are separated by 80 nucleotides EX28DRAFT_1980 and EX28DRAFT_1981 are separated by 57 nucleotides
EX28DRAFT_1977: EX28DRAFT_1977 - hemimethylated DNA binding domain, at 1,978,949 to 1,979,266
_1977
EX28DRAFT_1978: EX28DRAFT_1978 - Predicted CoA-binding protein, at 1,979,302 to 1,979,715
_1978
EX28DRAFT_1979: EX28DRAFT_1979 - Uncharacterized protein conserved in bacteria, at 1,979,889 to 1,980,551
_1979
EX28DRAFT_1980: EX28DRAFT_1980 - methylglyoxal synthase, at 1,980,632 to 1,981,090
_1980
EX28DRAFT_1981: EX28DRAFT_1981 - Superfamily I DNA and RNA helicases, at 1,981,148 to 1,983,202
_1981
Position (kb) 1979
1980
1981 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1978.903 kb on + strand at 1978.916 kb on + strand at 1978.916 kb on + strand at 1978.917 kb on - strand at 1978.923 kb on + strand at 1978.923 kb on + strand at 1978.923 kb on + strand at 1978.923 kb on + strand at 1978.924 kb on - strand at 1978.928 kb on - strand at 1979.147 kb on + strand, within EX28DRAFT_1977 at 1979.148 kb on - strand, within EX28DRAFT_1977 at 1979.265 kb on - strand at 1979.265 kb on - strand at 1979.534 kb on + strand, within EX28DRAFT_1978 at 1979.612 kb on + strand, within EX28DRAFT_1978 at 1979.700 kb on + strand at 1979.778 kb on + strand at 1979.807 kb on + strand at 1979.807 kb on + strand at 1979.808 kb on - strand at 1979.808 kb on - strand at 1979.808 kb on - strand at 1979.808 kb on - strand at 1979.813 kb on + strand at 1979.813 kb on + strand at 1979.814 kb on - strand at 1979.814 kb on - strand at 1979.814 kb on - strand at 1979.814 kb on - strand at 1979.815 kb on + strand at 1979.816 kb on - strand at 1979.816 kb on - strand at 1979.816 kb on - strand at 1979.816 kb on - strand at 1979.816 kb on - strand at 1979.816 kb on - strand at 1979.889 kb on - strand at 1979.986 kb on + strand, within EX28DRAFT_1979 at 1979.987 kb on - strand, within EX28DRAFT_1979 at 1980.117 kb on + strand, within EX28DRAFT_1979 at 1980.117 kb on + strand, within EX28DRAFT_1979 at 1980.117 kb on + strand, within EX28DRAFT_1979 at 1980.118 kb on - strand, within EX28DRAFT_1979 at 1980.118 kb on - strand, within EX28DRAFT_1979 at 1980.118 kb on - strand, within EX28DRAFT_1979 at 1980.118 kb on - strand, within EX28DRAFT_1979 at 1980.146 kb on + strand, within EX28DRAFT_1979 at 1980.282 kb on - strand, within EX28DRAFT_1979 at 1980.339 kb on + strand, within EX28DRAFT_1979 at 1980.339 kb on + strand, within EX28DRAFT_1979 at 1980.340 kb on - strand, within EX28DRAFT_1979 at 1980.549 kb on + strand at 1980.626 kb on - strand at 1980.626 kb on - strand at 1980.631 kb on + strand at 1980.697 kb on + strand, within EX28DRAFT_1980 at 1980.698 kb on - strand, within EX28DRAFT_1980 at 1980.713 kb on - strand, within EX28DRAFT_1980 at 1980.713 kb on - strand, within EX28DRAFT_1980 at 1980.713 kb on - strand, within EX28DRAFT_1980 at 1980.713 kb on - strand, within EX28DRAFT_1980 at 1980.970 kb on + strand, within EX28DRAFT_1980 at 1981.048 kb on + strand at 1981.049 kb on - strand at 1981.059 kb on - strand at 1981.127 kb on + strand at 1981.150 kb on - strand at 1981.177 kb on + strand at 1981.263 kb on + strand at 1981.392 kb on + strand, within EX28DRAFT_1981 at 1981.393 kb on - strand, within EX28DRAFT_1981 at 1981.430 kb on - strand, within EX28DRAFT_1981 at 1981.486 kb on + strand, within EX28DRAFT_1981 at 1981.487 kb on - strand, within EX28DRAFT_1981 at 1981.487 kb on - strand, within EX28DRAFT_1981 at 1981.516 kb on + strand, within EX28DRAFT_1981 at 1981.517 kb on - strand, within EX28DRAFT_1981
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 1,978,903 + +0.4 1,978,916 + +0.5 1,978,916 + -0.2 1,978,917 - +0.2 1,978,923 + -1.6 1,978,923 + -0.4 1,978,923 + -0.4 1,978,923 + +1.4 1,978,924 - -1.1 1,978,928 - -0.4 1,979,147 + EX28DRAFT_1977 0.62 -0.1 1,979,148 - EX28DRAFT_1977 0.63 -2.3 1,979,265 - -1.2 1,979,265 - -1.3 1,979,534 + EX28DRAFT_1978 0.56 -0.3 1,979,612 + EX28DRAFT_1978 0.75 +0.1 1,979,700 + -0.8 1,979,778 + +0.7 1,979,807 + +0.3 1,979,807 + -0.4 1,979,808 - +0.5 1,979,808 - +0.6 1,979,808 - -0.7 1,979,808 - +0.7 1,979,813 + -0.8 1,979,813 + -0.5 1,979,814 - +0.7 1,979,814 - +0.3 1,979,814 - -0.1 1,979,814 - +0.1 1,979,815 + +0.4 1,979,816 - +0.8 1,979,816 - -0.2 1,979,816 - +0.5 1,979,816 - +0.5 1,979,816 - +0.1 1,979,816 - -1.8 1,979,889 - +0.0 1,979,986 + EX28DRAFT_1979 0.15 -0.0 1,979,987 - EX28DRAFT_1979 0.15 +0.1 1,980,117 + EX28DRAFT_1979 0.34 +1.0 1,980,117 + EX28DRAFT_1979 0.34 -0.4 1,980,117 + EX28DRAFT_1979 0.34 -1.1 1,980,118 - EX28DRAFT_1979 0.35 +0.9 1,980,118 - EX28DRAFT_1979 0.35 -0.4 1,980,118 - EX28DRAFT_1979 0.35 +0.2 1,980,118 - EX28DRAFT_1979 0.35 -0.1 1,980,146 + EX28DRAFT_1979 0.39 -0.0 1,980,282 - EX28DRAFT_1979 0.59 +0.5 1,980,339 + EX28DRAFT_1979 0.68 +0.1 1,980,339 + EX28DRAFT_1979 0.68 -0.1 1,980,340 - EX28DRAFT_1979 0.68 +1.0 1,980,549 + +0.1 1,980,626 - +0.5 1,980,626 - -0.1 1,980,631 + -1.7 1,980,697 + EX28DRAFT_1980 0.14 +0.6 1,980,698 - EX28DRAFT_1980 0.14 +0.4 1,980,713 - EX28DRAFT_1980 0.18 -1.0 1,980,713 - EX28DRAFT_1980 0.18 -0.5 1,980,713 - EX28DRAFT_1980 0.18 +3.3 1,980,713 - EX28DRAFT_1980 0.18 +0.3 1,980,970 + EX28DRAFT_1980 0.74 -0.5 1,981,048 + +0.7 1,981,049 - +0.6 1,981,059 - -0.8 1,981,127 + +0.4 1,981,150 - +0.3 1,981,177 + +0.1 1,981,263 + +0.1 1,981,392 + EX28DRAFT_1981 0.12 +0.4 1,981,393 - EX28DRAFT_1981 0.12 -0.9 1,981,430 - EX28DRAFT_1981 0.14 -1.0 1,981,486 + EX28DRAFT_1981 0.16 -0.8 1,981,487 - EX28DRAFT_1981 0.16 -0.1 1,981,487 - EX28DRAFT_1981 0.16 -0.6 1,981,516 + EX28DRAFT_1981 0.18 +0.2 1,981,517 - EX28DRAFT_1981 0.18 +0.5
Or see this region's nucleotide sequence