Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1964 and EX28DRAFT_1965 are separated by 54 nucleotides EX28DRAFT_1965 and EX28DRAFT_1966 overlap by 4 nucleotides EX28DRAFT_1966 and EX28DRAFT_1967 are separated by 308 nucleotides
EX28DRAFT_1964: EX28DRAFT_1964 - Integral membrane protein, interacts with FtsH, at 1,967,392 to 1,968,051
_1964
EX28DRAFT_1965: EX28DRAFT_1965 - hypothetical protein, at 1,968,106 to 1,968,582
_1965
EX28DRAFT_1966: EX28DRAFT_1966 - DNA-binding winged-HTH domains, at 1,968,579 to 1,969,334
_1966
EX28DRAFT_1967: EX28DRAFT_1967 - hypothetical protein, at 1,969,643 to 1,970,386
_1967
Position (kb)
1968
1969
1970 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1967.617 kb on - strand, within EX28DRAFT_1964 at 1967.653 kb on + strand, within EX28DRAFT_1964 at 1967.680 kb on + strand, within EX28DRAFT_1964 at 1967.680 kb on + strand, within EX28DRAFT_1964 at 1967.738 kb on + strand, within EX28DRAFT_1964 at 1967.759 kb on - strand, within EX28DRAFT_1964 at 1967.963 kb on - strand, within EX28DRAFT_1964 at 1967.963 kb on - strand, within EX28DRAFT_1964 at 1967.964 kb on + strand, within EX28DRAFT_1964 at 1967.986 kb on + strand at 1967.986 kb on + strand at 1967.987 kb on - strand at 1967.998 kb on + strand at 1967.998 kb on + strand at 1967.998 kb on + strand at 1967.999 kb on - strand at 1968.062 kb on + strand at 1968.107 kb on + strand at 1968.123 kb on + strand at 1968.123 kb on + strand at 1968.125 kb on + strand at 1968.126 kb on - strand at 1968.126 kb on - strand at 1968.126 kb on - strand at 1968.188 kb on + strand, within EX28DRAFT_1965 at 1968.188 kb on + strand, within EX28DRAFT_1965 at 1968.189 kb on - strand, within EX28DRAFT_1965 at 1968.189 kb on - strand, within EX28DRAFT_1965 at 1968.192 kb on - strand, within EX28DRAFT_1965 at 1968.192 kb on - strand, within EX28DRAFT_1965 at 1968.237 kb on + strand, within EX28DRAFT_1965 at 1968.238 kb on - strand, within EX28DRAFT_1965 at 1968.270 kb on - strand, within EX28DRAFT_1965 at 1968.313 kb on - strand, within EX28DRAFT_1965 at 1968.371 kb on + strand, within EX28DRAFT_1965 at 1968.371 kb on + strand, within EX28DRAFT_1965 at 1968.371 kb on + strand, within EX28DRAFT_1965 at 1968.371 kb on + strand, within EX28DRAFT_1965 at 1968.371 kb on + strand, within EX28DRAFT_1965 at 1968.372 kb on - strand, within EX28DRAFT_1965 at 1968.405 kb on - strand, within EX28DRAFT_1965 at 1968.515 kb on + strand, within EX28DRAFT_1965 at 1968.519 kb on - strand, within EX28DRAFT_1965 at 1968.574 kb on + strand at 1968.600 kb on - strand at 1968.601 kb on + strand at 1968.602 kb on - strand at 1968.606 kb on + strand at 1968.606 kb on + strand at 1968.606 kb on + strand at 1968.607 kb on - strand at 1968.607 kb on - strand at 1968.607 kb on - strand at 1968.697 kb on + strand, within EX28DRAFT_1966 at 1968.698 kb on - strand, within EX28DRAFT_1966 at 1968.874 kb on + strand, within EX28DRAFT_1966 at 1968.874 kb on + strand, within EX28DRAFT_1966 at 1968.875 kb on - strand, within EX28DRAFT_1966 at 1969.127 kb on + strand, within EX28DRAFT_1966 at 1969.174 kb on - strand, within EX28DRAFT_1966 at 1969.209 kb on - strand, within EX28DRAFT_1966 at 1969.251 kb on + strand, within EX28DRAFT_1966 at 1969.254 kb on + strand, within EX28DRAFT_1966 at 1969.255 kb on - strand, within EX28DRAFT_1966 at 1969.281 kb on + strand at 1969.281 kb on + strand at 1969.282 kb on - strand at 1969.282 kb on - strand at 1969.658 kb on - strand at 1969.683 kb on - strand at 1969.846 kb on + strand, within EX28DRAFT_1967 at 1970.038 kb on + strand, within EX28DRAFT_1967 at 1970.170 kb on - strand, within EX28DRAFT_1967 at 1970.286 kb on + strand, within EX28DRAFT_1967 at 1970.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 1,967,617 - EX28DRAFT_1964 0.34 +1.6 1,967,653 + EX28DRAFT_1964 0.40 +1.1 1,967,680 + EX28DRAFT_1964 0.44 +0.3 1,967,680 + EX28DRAFT_1964 0.44 -0.0 1,967,738 + EX28DRAFT_1964 0.52 -1.7 1,967,759 - EX28DRAFT_1964 0.56 -0.3 1,967,963 - EX28DRAFT_1964 0.87 -1.6 1,967,963 - EX28DRAFT_1964 0.87 +0.6 1,967,964 + EX28DRAFT_1964 0.87 +0.2 1,967,986 + +0.2 1,967,986 + +0.4 1,967,987 - -0.6 1,967,998 + +0.5 1,967,998 + +0.6 1,967,998 + -0.4 1,967,999 - +0.2 1,968,062 + +0.3 1,968,107 + +0.5 1,968,123 + +0.5 1,968,123 + -0.5 1,968,125 + +0.5 1,968,126 - +0.1 1,968,126 - +0.6 1,968,126 - +1.1 1,968,188 + EX28DRAFT_1965 0.17 +0.3 1,968,188 + EX28DRAFT_1965 0.17 +1.1 1,968,189 - EX28DRAFT_1965 0.17 -0.5 1,968,189 - EX28DRAFT_1965 0.17 -0.5 1,968,192 - EX28DRAFT_1965 0.18 -0.3 1,968,192 - EX28DRAFT_1965 0.18 -0.5 1,968,237 + EX28DRAFT_1965 0.27 +1.3 1,968,238 - EX28DRAFT_1965 0.28 -0.9 1,968,270 - EX28DRAFT_1965 0.34 -1.2 1,968,313 - EX28DRAFT_1965 0.43 -2.0 1,968,371 + EX28DRAFT_1965 0.56 -0.2 1,968,371 + EX28DRAFT_1965 0.56 +0.1 1,968,371 + EX28DRAFT_1965 0.56 -0.6 1,968,371 + EX28DRAFT_1965 0.56 -0.4 1,968,371 + EX28DRAFT_1965 0.56 +0.0 1,968,372 - EX28DRAFT_1965 0.56 -0.0 1,968,405 - EX28DRAFT_1965 0.63 -0.9 1,968,515 + EX28DRAFT_1965 0.86 -0.2 1,968,519 - EX28DRAFT_1965 0.87 -0.1 1,968,574 + -0.7 1,968,600 - -1.3 1,968,601 + -0.2 1,968,602 - +0.2 1,968,606 + +0.8 1,968,606 + -0.1 1,968,606 + -0.4 1,968,607 - -0.0 1,968,607 - +0.5 1,968,607 - +0.0 1,968,697 + EX28DRAFT_1966 0.16 -0.5 1,968,698 - EX28DRAFT_1966 0.16 -0.1 1,968,874 + EX28DRAFT_1966 0.39 -0.4 1,968,874 + EX28DRAFT_1966 0.39 +0.1 1,968,875 - EX28DRAFT_1966 0.39 +1.8 1,969,127 + EX28DRAFT_1966 0.72 -3.4 1,969,174 - EX28DRAFT_1966 0.79 +0.0 1,969,209 - EX28DRAFT_1966 0.83 -0.1 1,969,251 + EX28DRAFT_1966 0.89 -0.1 1,969,254 + EX28DRAFT_1966 0.89 +0.9 1,969,255 - EX28DRAFT_1966 0.89 -0.2 1,969,281 + +0.6 1,969,281 + -0.3 1,969,282 - +1.3 1,969,282 - +0.2 1,969,658 - -0.4 1,969,683 - -0.3 1,969,846 + EX28DRAFT_1967 0.27 -1.1 1,970,038 + EX28DRAFT_1967 0.53 -0.1 1,970,170 - EX28DRAFT_1967 0.71 +0.0 1,970,286 + EX28DRAFT_1967 0.86 +0.0 1,970,321 - -2.7
Or see this region's nucleotide sequence